Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2217 and Pf6N2E2_2218 are separated by 107 nucleotides Pf6N2E2_2218 and Pf6N2E2_2219 are separated by 93 nucleotides Pf6N2E2_2219 and Pf6N2E2_2220 are separated by 129 nucleotides
Pf6N2E2_2217 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,628,296 to 2,629,579
_2217
Pf6N2E2_2218 - ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92), at 2,629,687 to 2,630,328
_2218
Pf6N2E2_2219 - Cell division trigger factor (EC 5.2.1.8), at 2,630,422 to 2,631,732
_2219
Pf6N2E2_2220 - Retron-type RNA-directed DNA polymerase (EC 2.7.7.49), at 2,631,862 to 2,633,565
_2220
Position (kb)
2630
2631
2632 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2629.536 kb on + strand at 2629.537 kb on - strand at 2629.676 kb on + strand at 2630.048 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.165 kb on + strand, within Pf6N2E2_2218 at 2630.219 kb on + strand, within Pf6N2E2_2218 at 2630.219 kb on + strand, within Pf6N2E2_2218 at 2630.220 kb on - strand, within Pf6N2E2_2218 at 2630.220 kb on - strand, within Pf6N2E2_2218 at 2630.349 kb on + strand at 2630.349 kb on + strand at 2630.350 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.365 kb on - strand at 2630.371 kb on - strand at 2630.393 kb on - strand at 2630.403 kb on - strand at 2630.466 kb on - strand at 2630.466 kb on - strand at 2630.509 kb on - strand at 2630.571 kb on - strand, within Pf6N2E2_2219 at 2631.072 kb on - strand, within Pf6N2E2_2219 at 2631.712 kb on - strand at 2631.736 kb on + strand at 2631.831 kb on - strand at 2631.946 kb on + strand at 2631.946 kb on + strand at 2631.947 kb on - strand at 2631.947 kb on - strand at 2631.947 kb on - strand at 2631.947 kb on - strand at 2631.962 kb on - strand at 2631.962 kb on - strand at 2632.038 kb on + strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.039 kb on - strand, within Pf6N2E2_2220 at 2632.042 kb on + strand, within Pf6N2E2_2220 at 2632.043 kb on - strand, within Pf6N2E2_2220 at 2632.043 kb on - strand, within Pf6N2E2_2220 at 2632.123 kb on - strand, within Pf6N2E2_2220 at 2632.123 kb on - strand, within Pf6N2E2_2220 at 2632.123 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.150 kb on - strand, within Pf6N2E2_2220 at 2632.170 kb on - strand, within Pf6N2E2_2220 at 2632.221 kb on - strand, within Pf6N2E2_2220 at 2632.221 kb on - strand, within Pf6N2E2_2220 at 2632.221 kb on - strand, within Pf6N2E2_2220 at 2632.305 kb on - strand, within Pf6N2E2_2220 at 2632.336 kb on - strand, within Pf6N2E2_2220 at 2632.336 kb on - strand, within Pf6N2E2_2220 at 2632.364 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.383 kb on - strand, within Pf6N2E2_2220 at 2632.396 kb on - strand, within Pf6N2E2_2220 at 2632.398 kb on - strand, within Pf6N2E2_2220 at 2632.428 kb on - strand, within Pf6N2E2_2220 at 2632.428 kb on - strand, within Pf6N2E2_2220 at 2632.428 kb on - strand, within Pf6N2E2_2220 at 2632.428 kb on - strand, within Pf6N2E2_2220 at 2632.428 kb on - strand, within Pf6N2E2_2220 at 2632.498 kb on - strand, within Pf6N2E2_2220 at 2632.498 kb on - strand, within Pf6N2E2_2220 at 2632.498 kb on - strand, within Pf6N2E2_2220 at 2632.513 kb on + strand, within Pf6N2E2_2220 at 2632.514 kb on - strand, within Pf6N2E2_2220 at 2632.514 kb on - strand, within Pf6N2E2_2220 at 2632.522 kb on - strand, within Pf6N2E2_2220 at 2632.522 kb on - strand, within Pf6N2E2_2220 at 2632.522 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.524 kb on - strand, within Pf6N2E2_2220 at 2632.575 kb on - strand, within Pf6N2E2_2220 at 2632.575 kb on - strand, within Pf6N2E2_2220 at 2632.575 kb on - strand, within Pf6N2E2_2220 at 2632.575 kb on - strand, within Pf6N2E2_2220 at 2632.575 kb on - strand, within Pf6N2E2_2220 at 2632.595 kb on - strand, within Pf6N2E2_2220 at 2632.595 kb on - strand at 2632.595 kb on - strand, within Pf6N2E2_2220 at 2632.615 kb on + strand, within Pf6N2E2_2220 at 2632.615 kb on + strand, within Pf6N2E2_2220 at 2632.615 kb on + strand, within Pf6N2E2_2220 at 2632.616 kb on - strand, within Pf6N2E2_2220 at 2632.616 kb on - strand, within Pf6N2E2_2220 at 2632.616 kb on - strand, within Pf6N2E2_2220 at 2632.616 kb on - strand, within Pf6N2E2_2220 at 2632.616 kb on - strand, within Pf6N2E2_2220 at 2632.618 kb on - strand, within Pf6N2E2_2220 at 2632.618 kb on - strand, within Pf6N2E2_2220 at 2632.618 kb on - strand, within Pf6N2E2_2220 at 2632.666 kb on + strand, within Pf6N2E2_2220 at 2632.666 kb on + strand, within Pf6N2E2_2220 at 2632.667 kb on - strand, within Pf6N2E2_2220 at 2632.667 kb on - strand, within Pf6N2E2_2220
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 2,629,536 + -2.3 2,629,537 - -2.6 2,629,676 + +0.8 2,630,048 + Pf6N2E2_2218 0.56 -0.8 2,630,093 + Pf6N2E2_2218 0.63 -1.1 2,630,093 + Pf6N2E2_2218 0.63 -1.5 2,630,093 + Pf6N2E2_2218 0.63 -0.3 2,630,093 + Pf6N2E2_2218 0.63 -0.1 2,630,093 + Pf6N2E2_2218 0.63 -1.1 2,630,094 - Pf6N2E2_2218 0.63 -0.8 2,630,094 - Pf6N2E2_2218 0.63 -2.0 2,630,094 - Pf6N2E2_2218 0.63 -0.8 2,630,094 - Pf6N2E2_2218 0.63 -1.8 2,630,094 - Pf6N2E2_2218 0.63 +0.5 2,630,094 - Pf6N2E2_2218 0.63 -2.0 2,630,165 + Pf6N2E2_2218 0.74 -1.3 2,630,219 + Pf6N2E2_2218 0.83 -1.3 2,630,219 + Pf6N2E2_2218 0.83 -1.5 2,630,220 - Pf6N2E2_2218 0.83 +0.8 2,630,220 - Pf6N2E2_2218 0.83 -1.5 2,630,349 + -0.8 2,630,349 + -2.2 2,630,350 - -1.8 2,630,354 - -0.7 2,630,354 - -1.5 2,630,354 - +0.3 2,630,354 - +0.5 2,630,354 - -1.1 2,630,365 - +0.0 2,630,371 - +0.4 2,630,393 - +0.9 2,630,403 - -0.3 2,630,466 - -1.3 2,630,466 - -1.6 2,630,509 - +0.9 2,630,571 - Pf6N2E2_2219 0.11 -0.5 2,631,072 - Pf6N2E2_2219 0.50 -1.0 2,631,712 - -1.1 2,631,736 + +0.0 2,631,831 - +1.0 2,631,946 + -0.1 2,631,946 + +1.1 2,631,947 - -2.7 2,631,947 - +1.2 2,631,947 - -1.7 2,631,947 - -0.9 2,631,962 - -0.9 2,631,962 - +0.2 2,632,038 + Pf6N2E2_2220 0.10 +1.5 2,632,039 - Pf6N2E2_2220 0.10 +0.4 2,632,039 - Pf6N2E2_2220 0.10 -1.2 2,632,039 - Pf6N2E2_2220 0.10 -0.1 2,632,039 - Pf6N2E2_2220 0.10 -1.2 2,632,039 - Pf6N2E2_2220 0.10 +0.5 2,632,039 - Pf6N2E2_2220 0.10 +0.4 2,632,039 - Pf6N2E2_2220 0.10 +1.9 2,632,039 - Pf6N2E2_2220 0.10 +0.0 2,632,039 - Pf6N2E2_2220 0.10 +1.2 2,632,039 - Pf6N2E2_2220 0.10 +0.0 2,632,039 - Pf6N2E2_2220 0.10 -0.1 2,632,039 - Pf6N2E2_2220 0.10 -0.3 2,632,039 - Pf6N2E2_2220 0.10 -0.7 2,632,039 - Pf6N2E2_2220 0.10 +1.5 2,632,039 - Pf6N2E2_2220 0.10 +1.3 2,632,042 + Pf6N2E2_2220 0.11 -0.7 2,632,043 - Pf6N2E2_2220 0.11 -0.0 2,632,043 - Pf6N2E2_2220 0.11 +0.5 2,632,123 - Pf6N2E2_2220 0.15 -0.3 2,632,123 - Pf6N2E2_2220 0.15 -1.9 2,632,123 - Pf6N2E2_2220 0.15 +0.0 2,632,150 - Pf6N2E2_2220 0.17 +0.5 2,632,150 - Pf6N2E2_2220 0.17 +1.7 2,632,150 - Pf6N2E2_2220 0.17 +1.2 2,632,150 - Pf6N2E2_2220 0.17 -0.6 2,632,150 - Pf6N2E2_2220 0.17 -0.3 2,632,150 - Pf6N2E2_2220 0.17 +0.5 2,632,150 - Pf6N2E2_2220 0.17 +2.2 2,632,170 - Pf6N2E2_2220 0.18 +0.6 2,632,221 - Pf6N2E2_2220 0.21 +0.5 2,632,221 - Pf6N2E2_2220 0.21 +0.6 2,632,221 - Pf6N2E2_2220 0.21 +1.6 2,632,305 - Pf6N2E2_2220 0.26 -0.7 2,632,336 - Pf6N2E2_2220 0.28 +0.1 2,632,336 - Pf6N2E2_2220 0.28 +0.7 2,632,364 - Pf6N2E2_2220 0.29 +1.0 2,632,383 - Pf6N2E2_2220 0.31 -1.6 2,632,383 - Pf6N2E2_2220 0.31 +0.2 2,632,383 - Pf6N2E2_2220 0.31 +1.1 2,632,383 - Pf6N2E2_2220 0.31 -0.4 2,632,383 - Pf6N2E2_2220 0.31 -0.3 2,632,383 - Pf6N2E2_2220 0.31 -0.6 2,632,383 - Pf6N2E2_2220 0.31 -0.0 2,632,383 - +2.5 2,632,383 - Pf6N2E2_2220 0.31 -0.8 2,632,383 - Pf6N2E2_2220 0.31 -0.2 2,632,396 - Pf6N2E2_2220 0.31 +0.5 2,632,398 - Pf6N2E2_2220 0.31 +1.1 2,632,428 - Pf6N2E2_2220 0.33 +0.7 2,632,428 - Pf6N2E2_2220 0.33 -0.3 2,632,428 - Pf6N2E2_2220 0.33 +1.1 2,632,428 - Pf6N2E2_2220 0.33 -2.7 2,632,428 - Pf6N2E2_2220 0.33 -0.0 2,632,498 - Pf6N2E2_2220 0.37 -0.3 2,632,498 - Pf6N2E2_2220 0.37 -1.2 2,632,498 - Pf6N2E2_2220 0.37 -1.3 2,632,513 + Pf6N2E2_2220 0.38 +0.5 2,632,514 - Pf6N2E2_2220 0.38 +0.3 2,632,514 - Pf6N2E2_2220 0.38 +0.8 2,632,522 - Pf6N2E2_2220 0.39 -1.1 2,632,522 - Pf6N2E2_2220 0.39 +0.4 2,632,522 - Pf6N2E2_2220 0.39 +0.2 2,632,524 - Pf6N2E2_2220 0.39 +1.3 2,632,524 - Pf6N2E2_2220 0.39 +2.6 2,632,524 - Pf6N2E2_2220 0.39 -0.5 2,632,524 - Pf6N2E2_2220 0.39 -0.8 2,632,524 - Pf6N2E2_2220 0.39 +0.5 2,632,524 - Pf6N2E2_2220 0.39 +0.1 2,632,524 - Pf6N2E2_2220 0.39 -0.0 2,632,524 - Pf6N2E2_2220 0.39 -0.4 2,632,524 - Pf6N2E2_2220 0.39 +0.2 2,632,524 - Pf6N2E2_2220 0.39 +0.6 2,632,524 - Pf6N2E2_2220 0.39 -0.8 2,632,575 - Pf6N2E2_2220 0.42 -0.2 2,632,575 - Pf6N2E2_2220 0.42 +0.5 2,632,575 - Pf6N2E2_2220 0.42 +0.3 2,632,575 - Pf6N2E2_2220 0.42 -0.9 2,632,575 - Pf6N2E2_2220 0.42 +0.4 2,632,595 - Pf6N2E2_2220 0.43 +2.1 2,632,595 - +1.5 2,632,595 - Pf6N2E2_2220 0.43 +0.2 2,632,615 + Pf6N2E2_2220 0.44 +0.4 2,632,615 + Pf6N2E2_2220 0.44 -1.0 2,632,615 + Pf6N2E2_2220 0.44 -0.3 2,632,616 - Pf6N2E2_2220 0.44 +0.8 2,632,616 - Pf6N2E2_2220 0.44 -0.3 2,632,616 - Pf6N2E2_2220 0.44 +0.4 2,632,616 - Pf6N2E2_2220 0.44 -0.7 2,632,616 - Pf6N2E2_2220 0.44 -0.8 2,632,618 - Pf6N2E2_2220 0.44 +1.0 2,632,618 - Pf6N2E2_2220 0.44 +1.0 2,632,618 - Pf6N2E2_2220 0.44 -0.1 2,632,666 + Pf6N2E2_2220 0.47 -0.4 2,632,666 + Pf6N2E2_2220 0.47 +0.3 2,632,667 - Pf6N2E2_2220 0.47 +0.1 2,632,667 - Pf6N2E2_2220 0.47 -0.7
Or see this region's nucleotide sequence