Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1861 and Pf6N2E2_1862 are separated by 334 nucleotides Pf6N2E2_1862 and Pf6N2E2_1863 are separated by 0 nucleotides Pf6N2E2_1863 and Pf6N2E2_1864 are separated by 416 nucleotides
Pf6N2E2_1861 - Ferredoxin reductase, at 2,204,360 to 2,205,595
_1861
Pf6N2E2_1862 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 2,205,930 to 2,206,424
_1862
Pf6N2E2_1863 - putative dioxygenase hydroxylase component, at 2,206,425 to 2,207,684
_1863
Pf6N2E2_1864 - Transcriptional regulator, TetR family, at 2,208,101 to 2,208,751
_1864
Position (kb)
2206
2207
2208 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2205.438 kb on + strand, within Pf6N2E2_1861 at 2205.439 kb on - strand, within Pf6N2E2_1861 at 2205.591 kb on + strand at 2205.592 kb on - strand at 2205.619 kb on + strand at 2205.620 kb on - strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.702 kb on - strand at 2206.031 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.194 kb on - strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.241 kb on + strand, within Pf6N2E2_1862 at 2206.295 kb on - strand, within Pf6N2E2_1862 at 2206.343 kb on + strand, within Pf6N2E2_1862 at 2206.427 kb on - strand at 2206.550 kb on - strand at 2206.597 kb on + strand, within Pf6N2E2_1863 at 2206.840 kb on + strand, within Pf6N2E2_1863 at 2206.841 kb on - strand, within Pf6N2E2_1863 at 2206.865 kb on - strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2207.251 kb on + strand, within Pf6N2E2_1863 at 2207.369 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863 at 2207.811 kb on - strand at 2207.850 kb on - strand at 2207.850 kb on - strand at 2207.988 kb on + strand at 2207.989 kb on - strand at 2207.989 kb on - strand at 2207.989 kb on - strand at 2208.043 kb on + strand at 2208.116 kb on + strand at 2208.176 kb on + strand, within Pf6N2E2_1864 at 2208.190 kb on + strand, within Pf6N2E2_1864 at 2208.191 kb on - strand, within Pf6N2E2_1864 at 2208.191 kb on - strand, within Pf6N2E2_1864 at 2208.269 kb on - strand, within Pf6N2E2_1864 at 2208.315 kb on + strand, within Pf6N2E2_1864 at 2208.564 kb on + strand, within Pf6N2E2_1864 at 2208.564 kb on + strand, within Pf6N2E2_1864 at 2208.565 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.569 kb on - strand, within Pf6N2E2_1864 at 2208.579 kb on + strand, within Pf6N2E2_1864
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 2,205,438 + Pf6N2E2_1861 0.87 +0.0 2,205,439 - Pf6N2E2_1861 0.87 -0.5 2,205,591 + -0.3 2,205,592 - +1.6 2,205,619 + +0.7 2,205,620 - +0.1 2,205,701 + +0.4 2,205,701 + -0.3 2,205,701 + -0.2 2,205,702 - -0.9 2,206,031 - Pf6N2E2_1862 0.20 +0.4 2,206,092 - Pf6N2E2_1862 0.33 -1.8 2,206,092 - Pf6N2E2_1862 0.33 +1.0 2,206,194 - Pf6N2E2_1862 0.53 -0.4 2,206,237 + Pf6N2E2_1862 0.62 +0.9 2,206,237 + Pf6N2E2_1862 0.62 +1.2 2,206,241 + Pf6N2E2_1862 0.63 -0.0 2,206,295 - Pf6N2E2_1862 0.74 -0.1 2,206,343 + Pf6N2E2_1862 0.83 +0.4 2,206,427 - +1.0 2,206,550 - -2.8 2,206,597 + Pf6N2E2_1863 0.14 -0.3 2,206,840 + Pf6N2E2_1863 0.33 -0.6 2,206,841 - Pf6N2E2_1863 0.33 -2.3 2,206,865 - Pf6N2E2_1863 0.35 -0.4 2,206,996 + Pf6N2E2_1863 0.45 +0.4 2,206,996 + Pf6N2E2_1863 0.45 -0.3 2,206,997 - Pf6N2E2_1863 0.45 -1.0 2,206,997 - Pf6N2E2_1863 0.45 +0.8 2,206,997 - Pf6N2E2_1863 0.45 -0.3 2,206,997 - Pf6N2E2_1863 0.45 -1.6 2,206,997 - Pf6N2E2_1863 0.45 -0.6 2,207,251 + Pf6N2E2_1863 0.66 -0.2 2,207,369 - Pf6N2E2_1863 0.75 +0.2 2,207,379 - Pf6N2E2_1863 0.76 -0.9 2,207,379 - Pf6N2E2_1863 0.76 +0.2 2,207,811 - -1.1 2,207,850 - -1.1 2,207,850 - -0.4 2,207,988 + -1.4 2,207,989 - -0.6 2,207,989 - +1.5 2,207,989 - +0.7 2,208,043 + +1.8 2,208,116 + -2.8 2,208,176 + Pf6N2E2_1864 0.12 -0.2 2,208,190 + Pf6N2E2_1864 0.14 -2.1 2,208,191 - Pf6N2E2_1864 0.14 -0.9 2,208,191 - Pf6N2E2_1864 0.14 -0.2 2,208,269 - Pf6N2E2_1864 0.26 -0.3 2,208,315 + Pf6N2E2_1864 0.33 -1.6 2,208,564 + Pf6N2E2_1864 0.71 -0.5 2,208,564 + Pf6N2E2_1864 0.71 +2.4 2,208,565 - Pf6N2E2_1864 0.71 +0.3 2,208,569 - Pf6N2E2_1864 0.72 +0.4 2,208,569 - Pf6N2E2_1864 0.72 -0.1 2,208,569 - Pf6N2E2_1864 0.72 -0.1 2,208,569 - Pf6N2E2_1864 0.72 -1.8 2,208,579 + Pf6N2E2_1864 0.73 +0.5
Or see this region's nucleotide sequence