Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1465 and Pf6N2E2_1466 are separated by 429 nucleotides Pf6N2E2_1466 and Pf6N2E2_1467 are separated by 20 nucleotides
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Pf6N2E2_1466 - NAD-dependent formate dehydrogenase delta subunit, at 1,714,633 to 1,714,830
_1466
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Position (kb)
1714
1715 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1713.645 kb on + strand, within Pf6N2E2_1465 at 1713.706 kb on - strand, within Pf6N2E2_1465 at 1713.755 kb on - strand, within Pf6N2E2_1465 at 1713.915 kb on - strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.095 kb on - strand at 1714.448 kb on - strand at 1714.448 kb on - strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.460 kb on - strand at 1714.460 kb on - strand at 1714.487 kb on - strand at 1714.611 kb on - strand at 1714.624 kb on + strand at 1714.624 kb on + strand at 1714.635 kb on - strand at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.834 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.988 kb on - strand at 1714.988 kb on - strand at 1715.068 kb on + strand at 1715.069 kb on - strand at 1715.257 kb on + strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.519 kb on - strand, within Pf6N2E2_1467
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 1,713,645 + Pf6N2E2_1465 0.58 +0.5 1,713,706 - Pf6N2E2_1465 0.63 +0.2 1,713,755 - Pf6N2E2_1465 0.67 -0.8 1,713,915 - Pf6N2E2_1465 0.78 -0.4 1,713,976 + Pf6N2E2_1465 0.83 +0.2 1,713,976 + Pf6N2E2_1465 0.83 +1.2 1,713,976 + Pf6N2E2_1465 0.83 -0.6 1,713,976 + Pf6N2E2_1465 0.83 +0.8 1,714,000 - Pf6N2E2_1465 0.85 +0.4 1,714,000 - Pf6N2E2_1465 0.85 +0.5 1,714,074 + -0.1 1,714,074 + +0.1 1,714,074 + +0.2 1,714,075 - +1.3 1,714,075 - -0.4 1,714,075 - +0.3 1,714,075 - +0.4 1,714,079 + +1.2 1,714,079 + +0.7 1,714,079 + -0.3 1,714,080 - +0.3 1,714,080 - +2.0 1,714,080 - -0.4 1,714,080 - +1.0 1,714,094 + +1.9 1,714,094 + -0.4 1,714,094 + -0.9 1,714,095 - -1.4 1,714,448 - -0.6 1,714,448 - +0.7 1,714,459 + +1.1 1,714,459 + +0.5 1,714,459 + -0.4 1,714,460 - -0.4 1,714,460 - +0.1 1,714,487 - -1.0 1,714,611 - -0.4 1,714,624 + -1.0 1,714,624 + -0.1 1,714,635 - -0.5 1,714,785 + Pf6N2E2_1466 0.77 +0.3 1,714,785 + Pf6N2E2_1466 0.77 -0.4 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,785 + Pf6N2E2_1466 0.77 +0.5 1,714,785 + Pf6N2E2_1466 0.77 -0.9 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,786 - Pf6N2E2_1466 0.77 -0.7 1,714,786 - Pf6N2E2_1466 0.77 -0.8 1,714,786 - Pf6N2E2_1466 0.77 +0.3 1,714,786 - Pf6N2E2_1466 0.77 +1.2 1,714,786 - Pf6N2E2_1466 0.77 +0.8 1,714,786 - Pf6N2E2_1466 0.77 +0.1 1,714,786 - Pf6N2E2_1466 0.77 -0.4 1,714,809 - Pf6N2E2_1466 0.89 -1.9 1,714,809 - Pf6N2E2_1466 0.89 -2.3 1,714,809 - Pf6N2E2_1466 0.89 -1.6 1,714,834 + +0.2 1,714,987 + +0.4 1,714,987 + +0.4 1,714,987 + -0.1 1,714,987 + +1.2 1,714,988 - -0.4 1,714,988 - +0.0 1,715,068 + +1.6 1,715,069 - +0.2 1,715,257 + Pf6N2E2_1467 0.14 +0.9 1,715,258 - Pf6N2E2_1467 0.14 -1.9 1,715,258 - Pf6N2E2_1467 0.14 -0.2 1,715,361 + Pf6N2E2_1467 0.18 -0.2 1,715,361 + Pf6N2E2_1467 0.18 +0.1 1,715,518 + Pf6N2E2_1467 0.23 -0.0 1,715,518 + Pf6N2E2_1467 0.23 -0.0 1,715,519 - Pf6N2E2_1467 0.23 -0.2
Or see this region's nucleotide sequence