Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1462 and Pf6N2E2_1463 are separated by 93 nucleotides Pf6N2E2_1463 and Pf6N2E2_1464 are separated by 308 nucleotides Pf6N2E2_1464 and Pf6N2E2_1465 are separated by 99 nucleotides
Pf6N2E2_1462 - Nicotinamidase/isochorismatase family protein, at 1,711,046 to 1,711,564
_1462
Pf6N2E2_1463 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 1,711,658 to 1,711,936
_1463
Pf6N2E2_1464 - hypothetical protein, at 1,712,245 to 1,712,763
_1464
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Position (kb)
1712
1713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1711.383 kb on - strand, within Pf6N2E2_1462 at 1711.387 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1712.225 kb on + strand at 1712.226 kb on - strand at 1712.265 kb on + strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.527 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.677 kb on + strand, within Pf6N2E2_1464 at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.747 kb on - strand at 1712.779 kb on + strand at 1712.780 kb on - strand at 1712.780 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.946 kb on + strand at 1712.967 kb on - strand at 1712.998 kb on - strand, within Pf6N2E2_1465 at 1713.009 kb on + strand, within Pf6N2E2_1465 at 1713.056 kb on + strand, within Pf6N2E2_1465 at 1713.066 kb on - strand, within Pf6N2E2_1465 at 1713.096 kb on + strand, within Pf6N2E2_1465 at 1713.097 kb on - strand, within Pf6N2E2_1465 at 1713.167 kb on - strand, within Pf6N2E2_1465 at 1713.543 kb on + strand, within Pf6N2E2_1465 at 1713.563 kb on + strand, within Pf6N2E2_1465 at 1713.564 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.612 kb on - strand, within Pf6N2E2_1465 at 1713.645 kb on + strand, within Pf6N2E2_1465 at 1713.706 kb on - strand, within Pf6N2E2_1465 at 1713.755 kb on - strand, within Pf6N2E2_1465
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 1,711,383 - Pf6N2E2_1462 0.65 -0.4 1,711,387 + Pf6N2E2_1462 0.66 +0.8 1,711,453 + Pf6N2E2_1462 0.78 -0.1 1,711,453 + Pf6N2E2_1462 0.78 +0.7 1,711,453 + Pf6N2E2_1462 0.78 +1.1 1,711,454 - Pf6N2E2_1462 0.79 +0.4 1,711,454 - Pf6N2E2_1462 0.79 +0.8 1,711,730 + Pf6N2E2_1463 0.26 +2.2 1,711,730 + Pf6N2E2_1463 0.26 -0.4 1,711,730 + Pf6N2E2_1463 0.26 -0.8 1,711,731 - Pf6N2E2_1463 0.26 -0.4 1,711,731 - Pf6N2E2_1463 0.26 -2.2 1,712,225 + -0.4 1,712,226 - -1.6 1,712,265 + -0.5 1,712,266 - +1.6 1,712,266 - +0.2 1,712,266 - -0.4 1,712,527 + Pf6N2E2_1464 0.54 -1.9 1,712,632 + Pf6N2E2_1464 0.75 -1.0 1,712,632 + Pf6N2E2_1464 0.75 -0.0 1,712,633 - Pf6N2E2_1464 0.75 -2.8 1,712,633 - Pf6N2E2_1464 0.75 -0.0 1,712,633 - Pf6N2E2_1464 0.75 -1.4 1,712,677 + Pf6N2E2_1464 0.83 +0.6 1,712,746 + -0.0 1,712,746 + +0.1 1,712,746 + +0.8 1,712,747 - -0.1 1,712,779 + -1.1 1,712,780 - +0.2 1,712,780 - +0.7 1,712,937 - -1.0 1,712,937 - +0.5 1,712,937 - +0.5 1,712,937 - -0.5 1,712,937 - +0.5 1,712,946 + -1.1 1,712,967 - -0.3 1,712,998 - Pf6N2E2_1465 0.10 +0.8 1,713,009 + Pf6N2E2_1465 0.11 +1.2 1,713,056 + Pf6N2E2_1465 0.14 -0.5 1,713,066 - Pf6N2E2_1465 0.15 +0.3 1,713,096 + Pf6N2E2_1465 0.17 -0.4 1,713,097 - Pf6N2E2_1465 0.17 +0.5 1,713,167 - Pf6N2E2_1465 0.23 +0.3 1,713,543 + Pf6N2E2_1465 0.51 -1.2 1,713,563 + Pf6N2E2_1465 0.52 +1.8 1,713,564 - Pf6N2E2_1465 0.52 +0.5 1,713,570 - Pf6N2E2_1465 0.53 -0.6 1,713,570 - Pf6N2E2_1465 0.53 -0.7 1,713,611 + Pf6N2E2_1465 0.56 -0.2 1,713,611 + Pf6N2E2_1465 0.56 -0.5 1,713,612 - Pf6N2E2_1465 0.56 -0.1 1,713,645 + Pf6N2E2_1465 0.58 +0.5 1,713,706 - Pf6N2E2_1465 0.63 +0.2 1,713,755 - Pf6N2E2_1465 0.67 -0.8
Or see this region's nucleotide sequence