Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1172 and Pf6N2E2_1173 are separated by 281 nucleotides Pf6N2E2_1173 and Pf6N2E2_1174 are separated by 278 nucleotides
Pf6N2E2_1172 - Short-chain dehydrogenase/reductase SDR, at 1,372,316 to 1,373,089
_1172
Pf6N2E2_1173 - hypothetical protein, at 1,373,371 to 1,373,685
_1173
Pf6N2E2_1174 - hypothetical protein, at 1,373,964 to 1,375,022
_1174
Position (kb)
1373
1374 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.383 kb on - strand at 1372.383 kb on - strand at 1372.393 kb on + strand at 1372.394 kb on - strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.404 kb on - strand at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.511 kb on - strand, within Pf6N2E2_1172 at 1372.614 kb on + strand, within Pf6N2E2_1172 at 1372.614 kb on + strand, within Pf6N2E2_1172 at 1372.623 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.659 kb on - strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.955 kb on - strand, within Pf6N2E2_1172 at 1372.971 kb on + strand, within Pf6N2E2_1172 at 1373.066 kb on - strand at 1373.066 kb on - strand at 1373.107 kb on - strand at 1373.107 kb on - strand at 1373.129 kb on + strand at 1373.130 kb on - strand at 1373.161 kb on + strand at 1373.228 kb on + strand at 1373.285 kb on - strand at 1373.288 kb on + strand at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.518 kb on + strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.725 kb on + strand at 1373.791 kb on - strand at 1373.965 kb on + strand at 1374.139 kb on + strand, within Pf6N2E2_1174 at 1374.208 kb on + strand, within Pf6N2E2_1174 at 1374.208 kb on + strand, within Pf6N2E2_1174 at 1374.234 kb on + strand, within Pf6N2E2_1174 at 1374.337 kb on + strand, within Pf6N2E2_1174 at 1374.456 kb on + strand, within Pf6N2E2_1174 at 1374.457 kb on - strand, within Pf6N2E2_1174 at 1374.469 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.595 kb on - strand, within Pf6N2E2_1174 at 1374.681 kb on - strand, within Pf6N2E2_1174
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 1,372,382 + -0.2 1,372,382 + +0.1 1,372,382 + +0.4 1,372,382 + -1.8 1,372,382 + -0.8 1,372,383 - -0.7 1,372,383 - -1.2 1,372,393 + +1.0 1,372,394 - Pf6N2E2_1172 0.10 -0.0 1,372,403 + Pf6N2E2_1172 0.11 +0.1 1,372,403 + Pf6N2E2_1172 0.11 -0.9 1,372,403 + Pf6N2E2_1172 0.11 +2.3 1,372,403 + Pf6N2E2_1172 0.11 +0.1 1,372,404 - +0.9 1,372,404 - Pf6N2E2_1172 0.11 -0.1 1,372,404 - Pf6N2E2_1172 0.11 -0.8 1,372,404 - Pf6N2E2_1172 0.11 +1.8 1,372,511 - Pf6N2E2_1172 0.25 -1.0 1,372,614 + Pf6N2E2_1172 0.39 -0.7 1,372,614 + Pf6N2E2_1172 0.39 -0.2 1,372,623 + Pf6N2E2_1172 0.40 +0.1 1,372,654 + Pf6N2E2_1172 0.44 +0.2 1,372,654 + Pf6N2E2_1172 0.44 -0.3 1,372,654 + Pf6N2E2_1172 0.44 +0.3 1,372,655 - Pf6N2E2_1172 0.44 +1.3 1,372,655 - Pf6N2E2_1172 0.44 +0.3 1,372,655 - Pf6N2E2_1172 0.44 +0.8 1,372,655 - Pf6N2E2_1172 0.44 -0.5 1,372,655 - Pf6N2E2_1172 0.44 -0.1 1,372,658 + Pf6N2E2_1172 0.44 -0.4 1,372,658 + Pf6N2E2_1172 0.44 -0.7 1,372,658 + Pf6N2E2_1172 0.44 +1.3 1,372,659 - Pf6N2E2_1172 0.44 -1.8 1,372,954 + Pf6N2E2_1172 0.82 -0.3 1,372,954 + Pf6N2E2_1172 0.82 -0.8 1,372,954 + Pf6N2E2_1172 0.82 +1.5 1,372,955 - Pf6N2E2_1172 0.83 -0.8 1,372,971 + Pf6N2E2_1172 0.85 -0.4 1,373,066 - -0.0 1,373,066 - +0.1 1,373,107 - -0.8 1,373,107 - +1.9 1,373,129 + -0.1 1,373,130 - +0.2 1,373,161 + +1.2 1,373,228 + -1.2 1,373,285 - +0.7 1,373,288 + +1.1 1,373,472 - Pf6N2E2_1173 0.32 -2.7 1,373,472 - Pf6N2E2_1173 0.32 -2.5 1,373,518 + Pf6N2E2_1173 0.47 +0.5 1,373,519 - Pf6N2E2_1173 0.47 -0.4 1,373,519 - Pf6N2E2_1173 0.47 -0.2 1,373,725 + +0.6 1,373,791 - -0.4 1,373,965 + +0.9 1,374,139 + Pf6N2E2_1174 0.17 +0.0 1,374,208 + Pf6N2E2_1174 0.23 +0.9 1,374,208 + Pf6N2E2_1174 0.23 +0.1 1,374,234 + Pf6N2E2_1174 0.25 +1.0 1,374,337 + Pf6N2E2_1174 0.35 -0.4 1,374,456 + Pf6N2E2_1174 0.46 +0.1 1,374,457 - Pf6N2E2_1174 0.47 +0.2 1,374,469 + Pf6N2E2_1174 0.48 +1.2 1,374,488 + Pf6N2E2_1174 0.49 +1.2 1,374,488 + Pf6N2E2_1174 0.49 -1.7 1,374,595 - Pf6N2E2_1174 0.60 -1.0 1,374,681 - Pf6N2E2_1174 0.68 -0.1
Or see this region's nucleotide sequence