Experiment: pyrophosphate 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1110 and Pf6N2E2_1111 are separated by 198 nucleotides Pf6N2E2_1111 and Pf6N2E2_1112 are separated by 2 nucleotides Pf6N2E2_1112 and Pf6N2E2_1113 are separated by 154 nucleotides
Pf6N2E2_1110 - FIG00964414: hypothetical protein, at 1,302,159 to 1,302,842
_1110
Pf6N2E2_1111 - cell wall degradation protein, at 1,303,041 to 1,304,567
_1111
Pf6N2E2_1112 - NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 1,304,570 to 1,305,199
_1112
Pf6N2E2_1113 - Transcriptional regulator, TetR family, at 1,305,354 to 1,305,905
_1113
Position (kb)
1303
1304
1305 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1302.045 kb on + strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.071 kb on - strand at 1302.071 kb on - strand at 1302.160 kb on + strand at 1302.183 kb on - strand at 1302.376 kb on + strand, within Pf6N2E2_1110 at 1302.476 kb on - strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.591 kb on + strand, within Pf6N2E2_1110 at 1302.592 kb on - strand, within Pf6N2E2_1110 at 1302.951 kb on + strand at 1302.952 kb on - strand at 1302.998 kb on - strand at 1303.026 kb on + strand at 1303.026 kb on + strand at 1303.026 kb on + strand at 1303.027 kb on - strand at 1303.039 kb on + strand at 1303.040 kb on - strand at 1303.183 kb on - strand at 1303.288 kb on - strand, within Pf6N2E2_1111 at 1303.354 kb on - strand, within Pf6N2E2_1111 at 1303.530 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.711 kb on - strand, within Pf6N2E2_1111 at 1303.929 kb on + strand, within Pf6N2E2_1111 at 1303.930 kb on - strand, within Pf6N2E2_1111 at 1303.930 kb on - strand, within Pf6N2E2_1111 at 1304.265 kb on + strand, within Pf6N2E2_1111 at 1304.266 kb on - strand, within Pf6N2E2_1111 at 1304.505 kb on + strand at 1304.505 kb on + strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.520 kb on + strand at 1304.520 kb on + strand at 1304.521 kb on - strand at 1304.766 kb on + strand, within Pf6N2E2_1112 at 1305.238 kb on + strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.249 kb on + strand at 1305.453 kb on + strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 0.0025 M remove 1,302,045 + -0.4 1,302,046 - +0.6 1,302,046 - -1.8 1,302,046 - +2.0 1,302,046 - +1.0 1,302,071 - +1.0 1,302,071 - +0.7 1,302,160 + +1.8 1,302,183 - -0.8 1,302,376 + Pf6N2E2_1110 0.32 +1.0 1,302,476 - Pf6N2E2_1110 0.46 +0.6 1,302,514 + Pf6N2E2_1110 0.52 -1.0 1,302,514 + Pf6N2E2_1110 0.52 +0.0 1,302,515 - Pf6N2E2_1110 0.52 +0.7 1,302,515 - Pf6N2E2_1110 0.52 -0.9 1,302,515 - Pf6N2E2_1110 0.52 +0.5 1,302,591 + Pf6N2E2_1110 0.63 -0.4 1,302,592 - Pf6N2E2_1110 0.63 +0.3 1,302,951 + -0.8 1,302,952 - -1.5 1,302,998 - -0.1 1,303,026 + -0.8 1,303,026 + +1.5 1,303,026 + -0.4 1,303,027 - -0.5 1,303,039 + -1.0 1,303,040 - +0.2 1,303,183 - -2.1 1,303,288 - Pf6N2E2_1111 0.16 -0.2 1,303,354 - Pf6N2E2_1111 0.20 +0.6 1,303,530 + Pf6N2E2_1111 0.32 -2.0 1,303,710 + Pf6N2E2_1111 0.44 -1.1 1,303,710 + Pf6N2E2_1111 0.44 +0.7 1,303,710 + Pf6N2E2_1111 0.44 -0.6 1,303,711 - Pf6N2E2_1111 0.44 +0.4 1,303,929 + Pf6N2E2_1111 0.58 -0.8 1,303,930 - Pf6N2E2_1111 0.58 +0.1 1,303,930 - Pf6N2E2_1111 0.58 -0.5 1,304,265 + Pf6N2E2_1111 0.80 -0.8 1,304,266 - Pf6N2E2_1111 0.80 -0.3 1,304,505 + -0.0 1,304,505 + +0.7 1,304,506 - +0.1 1,304,506 - -0.5 1,304,506 - +0.4 1,304,520 + -1.0 1,304,520 + -0.7 1,304,521 - -1.8 1,304,766 + Pf6N2E2_1112 0.31 +2.2 1,305,238 + +0.4 1,305,239 - +0.6 1,305,239 - -0.1 1,305,239 - +0.6 1,305,249 + -1.6 1,305,453 + Pf6N2E2_1113 0.18 +1.0 1,305,496 - Pf6N2E2_1113 0.26 +1.4 1,305,496 - Pf6N2E2_1113 0.26 -0.1
Or see this region's nucleotide sequence