Experiment: L-Arginine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3207 and Pf1N1B4_3208 are separated by 145 nucleotides Pf1N1B4_3208 and Pf1N1B4_3209 overlap by 4 nucleotides Pf1N1B4_3209 and Pf1N1B4_3210 overlap by 4 nucleotides Pf1N1B4_3210 and Pf1N1B4_3211 are separated by 25 nucleotides
Pf1N1B4_3207 - Transcriptional regulator, LysR family, at 3,590,870 to 3,591,778
_3207
Pf1N1B4_3208 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,591,924 to 3,592,427
_3208
Pf1N1B4_3209 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,592,424 to 3,593,182
_3209
Pf1N1B4_3210 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,593,179 to 3,594,033
_3210
Pf1N1B4_3211 - Putative signal peptide protein, at 3,594,059 to 3,594,364
_3211
Position (kb)
3592
3593
3594 Strain fitness (log2 ratio)
-2
-1
0
1 at 3591.453 kb on - strand, within Pf1N1B4_3207 at 3591.476 kb on + strand, within Pf1N1B4_3207 at 3591.476 kb on + strand at 3591.477 kb on - strand, within Pf1N1B4_3207 at 3591.495 kb on + strand, within Pf1N1B4_3207 at 3591.495 kb on + strand, within Pf1N1B4_3207 at 3591.496 kb on - strand, within Pf1N1B4_3207 at 3591.536 kb on + strand at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.536 kb on + strand, within Pf1N1B4_3207 at 3591.537 kb on - strand, within Pf1N1B4_3207 at 3591.537 kb on - strand, within Pf1N1B4_3207 at 3591.593 kb on + strand, within Pf1N1B4_3207 at 3591.593 kb on + strand, within Pf1N1B4_3207 at 3591.594 kb on - strand at 3591.594 kb on - strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.671 kb on + strand, within Pf1N1B4_3207 at 3591.672 kb on - strand, within Pf1N1B4_3207 at 3591.776 kb on + strand at 3591.776 kb on + strand at 3591.777 kb on - strand at 3591.853 kb on + strand at 3591.853 kb on + strand at 3591.854 kb on - strand at 3591.854 kb on - strand at 3592.009 kb on + strand, within Pf1N1B4_3208 at 3592.009 kb on + strand, within Pf1N1B4_3208 at 3592.009 kb on + strand, within Pf1N1B4_3208 at 3592.009 kb on + strand, within Pf1N1B4_3208 at 3592.010 kb on - strand, within Pf1N1B4_3208 at 3592.010 kb on - strand, within Pf1N1B4_3208 at 3592.086 kb on - strand, within Pf1N1B4_3208 at 3592.207 kb on + strand, within Pf1N1B4_3208 at 3592.207 kb on + strand, within Pf1N1B4_3208 at 3592.445 kb on + strand at 3592.445 kb on + strand at 3592.445 kb on + strand at 3592.445 kb on + strand at 3592.445 kb on + strand at 3592.446 kb on - strand at 3592.446 kb on - strand at 3592.869 kb on + strand, within Pf1N1B4_3209 at 3592.869 kb on + strand, within Pf1N1B4_3209 at 3592.869 kb on + strand, within Pf1N1B4_3209 at 3592.870 kb on - strand, within Pf1N1B4_3209 at 3592.870 kb on - strand, within Pf1N1B4_3209 at 3592.870 kb on - strand, within Pf1N1B4_3209 at 3592.870 kb on - strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.968 kb on + strand, within Pf1N1B4_3209 at 3592.969 kb on - strand, within Pf1N1B4_3209 at 3592.969 kb on - strand at 3592.969 kb on - strand, within Pf1N1B4_3209 at 3593.062 kb on + strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.255 kb on - strand at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.388 kb on + strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.489 kb on + strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.646 kb on + strand, within Pf1N1B4_3210 at 3593.647 kb on - strand, within Pf1N1B4_3210 at 3593.682 kb on - strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.827 kb on - strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand at 3593.912 kb on + strand at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arginine (N) remove 3,591,453 - Pf1N1B4_3207 0.64 +0.1 3,591,476 + Pf1N1B4_3207 0.67 -0.2 3,591,476 + -0.3 3,591,477 - Pf1N1B4_3207 0.67 +0.2 3,591,495 + Pf1N1B4_3207 0.69 +0.6 3,591,495 + Pf1N1B4_3207 0.69 +0.1 3,591,496 - Pf1N1B4_3207 0.69 +0.8 3,591,536 + -1.3 3,591,536 + Pf1N1B4_3207 0.73 -0.0 3,591,536 + Pf1N1B4_3207 0.73 -0.2 3,591,536 + Pf1N1B4_3207 0.73 -0.7 3,591,537 - Pf1N1B4_3207 0.73 -0.5 3,591,537 - Pf1N1B4_3207 0.73 -0.3 3,591,593 + Pf1N1B4_3207 0.80 -0.3 3,591,593 + Pf1N1B4_3207 0.80 +1.0 3,591,594 - +1.6 3,591,594 - Pf1N1B4_3207 0.80 -1.0 3,591,671 + Pf1N1B4_3207 0.88 -1.2 3,591,671 + Pf1N1B4_3207 0.88 +0.1 3,591,671 + Pf1N1B4_3207 0.88 -0.9 3,591,671 + Pf1N1B4_3207 0.88 -0.4 3,591,672 - Pf1N1B4_3207 0.88 +0.6 3,591,776 + +0.2 3,591,776 + -0.2 3,591,777 - -0.2 3,591,853 + -1.8 3,591,853 + -0.2 3,591,854 - +0.0 3,591,854 - -0.5 3,592,009 + Pf1N1B4_3208 0.17 -0.2 3,592,009 + Pf1N1B4_3208 0.17 -1.5 3,592,009 + Pf1N1B4_3208 0.17 -0.3 3,592,009 + Pf1N1B4_3208 0.17 -0.7 3,592,010 - Pf1N1B4_3208 0.17 +0.4 3,592,010 - Pf1N1B4_3208 0.17 -0.0 3,592,086 - Pf1N1B4_3208 0.32 -0.4 3,592,207 + Pf1N1B4_3208 0.56 -0.2 3,592,207 + Pf1N1B4_3208 0.56 -0.3 3,592,445 + +0.7 3,592,445 + -0.3 3,592,445 + +0.2 3,592,445 + -1.4 3,592,445 + +0.5 3,592,446 - -2.0 3,592,446 - -1.0 3,592,869 + Pf1N1B4_3209 0.59 -0.8 3,592,869 + Pf1N1B4_3209 0.59 -0.3 3,592,869 + Pf1N1B4_3209 0.59 -0.3 3,592,870 - Pf1N1B4_3209 0.59 -0.2 3,592,870 - Pf1N1B4_3209 0.59 -0.6 3,592,870 - Pf1N1B4_3209 0.59 +1.3 3,592,870 - Pf1N1B4_3209 0.59 +0.1 3,592,968 + Pf1N1B4_3209 0.72 +1.2 3,592,968 + Pf1N1B4_3209 0.72 +0.7 3,592,968 + Pf1N1B4_3209 0.72 +1.0 3,592,968 + Pf1N1B4_3209 0.72 -0.9 3,592,968 + Pf1N1B4_3209 0.72 -0.5 3,592,968 + Pf1N1B4_3209 0.72 -0.1 3,592,968 + Pf1N1B4_3209 0.72 +1.2 3,592,968 + Pf1N1B4_3209 0.72 +0.5 3,592,968 + Pf1N1B4_3209 0.72 -0.9 3,592,968 + Pf1N1B4_3209 0.72 +0.2 3,592,968 + Pf1N1B4_3209 0.72 +0.0 3,592,968 + Pf1N1B4_3209 0.72 +0.2 3,592,968 + Pf1N1B4_3209 0.72 +0.1 3,592,968 + Pf1N1B4_3209 0.72 -1.8 3,592,969 - Pf1N1B4_3209 0.72 +0.0 3,592,969 - +0.6 3,592,969 - Pf1N1B4_3209 0.72 +0.9 3,593,062 + Pf1N1B4_3209 0.84 -0.5 3,593,063 - Pf1N1B4_3209 0.84 -0.8 3,593,063 - Pf1N1B4_3209 0.84 -0.7 3,593,073 + Pf1N1B4_3209 0.86 +0.0 3,593,073 + Pf1N1B4_3209 0.86 +0.0 3,593,255 - -0.7 3,593,315 + Pf1N1B4_3210 0.16 +0.2 3,593,315 + Pf1N1B4_3210 0.16 -0.3 3,593,388 + Pf1N1B4_3210 0.24 +0.9 3,593,389 - Pf1N1B4_3210 0.25 -0.2 3,593,389 - Pf1N1B4_3210 0.25 +0.2 3,593,451 - Pf1N1B4_3210 0.32 -0.3 3,593,451 - Pf1N1B4_3210 0.32 -1.1 3,593,489 + Pf1N1B4_3210 0.36 +0.7 3,593,490 - Pf1N1B4_3210 0.36 -0.6 3,593,490 - Pf1N1B4_3210 0.36 -0.7 3,593,490 - Pf1N1B4_3210 0.36 +0.0 3,593,490 - Pf1N1B4_3210 0.36 -0.7 3,593,588 + Pf1N1B4_3210 0.48 -0.2 3,593,588 + Pf1N1B4_3210 0.48 -0.5 3,593,589 - Pf1N1B4_3210 0.48 +0.5 3,593,589 - Pf1N1B4_3210 0.48 +0.0 3,593,589 - Pf1N1B4_3210 0.48 -0.4 3,593,646 + Pf1N1B4_3210 0.55 +0.0 3,593,647 - Pf1N1B4_3210 0.55 -0.3 3,593,682 - Pf1N1B4_3210 0.59 +0.7 3,593,826 + Pf1N1B4_3210 0.76 -0.9 3,593,826 + Pf1N1B4_3210 0.76 -0.4 3,593,826 + Pf1N1B4_3210 0.76 -0.1 3,593,826 + Pf1N1B4_3210 0.76 -0.7 3,593,827 - Pf1N1B4_3210 0.76 +1.0 3,593,858 + Pf1N1B4_3210 0.79 -1.3 3,593,858 + Pf1N1B4_3210 0.79 +0.0 3,593,858 + Pf1N1B4_3210 0.79 +0.0 3,593,912 + Pf1N1B4_3210 0.86 -1.0 3,593,912 + +1.0 3,593,912 + -0.6 3,593,912 + Pf1N1B4_3210 0.86 -1.0 3,593,913 - Pf1N1B4_3210 0.86 -1.0 3,593,913 - Pf1N1B4_3210 0.86 +0.0 3,593,913 - Pf1N1B4_3210 0.86 -0.8 3,594,075 + -0.1 3,594,075 + +0.2 3,594,075 + -2.2 3,594,076 - -0.8 3,594,076 - +0.2 3,594,076 - -0.4 3,594,076 - -0.6 3,594,076 - +0.6 3,594,076 - -0.5
Or see this region's nucleotide sequence