Experiment: Survival; Treatment=UGA synthetic gw pH5.0; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21260 and IAI47_21265 are separated by 112 nucleotides IAI47_21265 and IAI47_21270 overlap by 4 nucleotides IAI47_21270 and IAI47_21275 are separated by 9 nucleotides IAI47_21275 and IAI47_21280 are separated by 6 nucleotides
IAI47_21260: IAI47_21260 - glutathione S-transferase, at 508,902 to 509,564
_21260
IAI47_21265: IAI47_21265 - ATP-binding cassette domain-containing protein, at 509,677 to 510,480
_21265
IAI47_21270: IAI47_21270 - ABC transporter permease subunit, at 510,477 to 511,163
_21270
IAI47_21275: IAI47_21275 - ABC transporter permease subunit, at 511,173 to 511,898
_21275
IAI47_21280: IAI47_21280 - amino acid ABC transporter substrate-binding protein, at 511,905 to 512,753
_21280
Position (kb) 510
511
512 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 509.484 kb on + strand, within IAI47_21260 at 509.511 kb on + strand at 509.511 kb on + strand at 509.512 kb on - strand at 509.562 kb on + strand at 509.563 kb on - strand at 509.573 kb on + strand at 509.574 kb on - strand at 509.581 kb on + strand at 509.581 kb on + strand at 509.582 kb on - strand at 509.582 kb on - strand at 509.582 kb on - strand at 509.651 kb on + strand at 509.712 kb on + strand at 509.712 kb on + strand at 509.869 kb on - strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.877 kb on + strand, within IAI47_21265 at 509.878 kb on - strand, within IAI47_21265 at 509.878 kb on - strand, within IAI47_21265 at 509.878 kb on - strand, within IAI47_21265 at 509.878 kb on - strand, within IAI47_21265 at 509.878 kb on - strand, within IAI47_21265 at 509.898 kb on + strand, within IAI47_21265 at 509.898 kb on + strand, within IAI47_21265 at 509.899 kb on - strand, within IAI47_21265 at 509.899 kb on - strand, within IAI47_21265 at 509.899 kb on - strand, within IAI47_21265 at 509.902 kb on + strand, within IAI47_21265 at 509.902 kb on + strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.903 kb on - strand, within IAI47_21265 at 509.911 kb on + strand, within IAI47_21265 at 509.912 kb on - strand, within IAI47_21265 at 509.914 kb on + strand, within IAI47_21265 at 509.914 kb on + strand, within IAI47_21265 at 509.915 kb on - strand, within IAI47_21265 at 509.915 kb on - strand, within IAI47_21265 at 509.915 kb on - strand, within IAI47_21265 at 509.915 kb on - strand, within IAI47_21265 at 510.028 kb on + strand, within IAI47_21265 at 510.059 kb on + strand, within IAI47_21265 at 510.059 kb on + strand, within IAI47_21265 at 510.073 kb on - strand, within IAI47_21265 at 510.108 kb on + strand, within IAI47_21265 at 510.109 kb on - strand, within IAI47_21265 at 510.265 kb on - strand, within IAI47_21265 at 510.279 kb on + strand, within IAI47_21265 at 510.280 kb on - strand, within IAI47_21265 at 510.331 kb on - strand, within IAI47_21265 at 510.345 kb on + strand, within IAI47_21265 at 510.345 kb on + strand, within IAI47_21265 at 510.346 kb on - strand, within IAI47_21265 at 510.346 kb on - strand, within IAI47_21265 at 510.437 kb on + strand at 510.437 kb on + strand at 510.438 kb on - strand at 510.438 kb on - strand at 510.468 kb on + strand at 510.468 kb on + strand at 510.468 kb on + strand at 510.468 kb on + strand at 510.468 kb on + strand at 510.469 kb on - strand at 510.469 kb on - strand at 510.496 kb on + strand at 510.496 kb on + strand at 510.497 kb on - strand at 510.497 kb on - strand at 510.499 kb on + strand at 510.499 kb on + strand at 510.499 kb on + strand at 510.499 kb on + strand at 510.500 kb on - strand at 510.507 kb on + strand at 510.536 kb on + strand at 510.536 kb on + strand at 510.548 kb on + strand, within IAI47_21270 at 510.548 kb on + strand, within IAI47_21270 at 510.548 kb on + strand, within IAI47_21270 at 510.548 kb on + strand, within IAI47_21270 at 510.548 kb on + strand, within IAI47_21270 at 510.549 kb on - strand, within IAI47_21270 at 510.549 kb on - strand, within IAI47_21270 at 510.549 kb on - strand, within IAI47_21270 at 510.549 kb on - strand, within IAI47_21270 at 510.549 kb on - strand, within IAI47_21270 at 510.556 kb on - strand, within IAI47_21270 at 510.562 kb on + strand, within IAI47_21270 at 510.586 kb on - strand, within IAI47_21270 at 510.586 kb on - strand, within IAI47_21270 at 510.703 kb on + strand, within IAI47_21270 at 510.704 kb on - strand, within IAI47_21270 at 510.712 kb on - strand, within IAI47_21270 at 510.761 kb on - strand, within IAI47_21270 at 510.844 kb on + strand, within IAI47_21270 at 510.844 kb on + strand, within IAI47_21270 at 510.844 kb on + strand, within IAI47_21270 at 510.845 kb on - strand, within IAI47_21270 at 510.845 kb on - strand, within IAI47_21270 at 510.857 kb on + strand, within IAI47_21270 at 510.887 kb on - strand, within IAI47_21270 at 510.897 kb on + strand, within IAI47_21270 at 510.897 kb on + strand, within IAI47_21270 at 510.897 kb on + strand, within IAI47_21270 at 510.897 kb on + strand, within IAI47_21270 at 510.897 kb on + strand, within IAI47_21270 at 510.898 kb on - strand, within IAI47_21270 at 510.898 kb on - strand, within IAI47_21270 at 510.918 kb on - strand, within IAI47_21270 at 510.918 kb on - strand, within IAI47_21270 at 510.918 kb on - strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.953 kb on - strand, within IAI47_21270 at 510.959 kb on + strand, within IAI47_21270 at 510.959 kb on + strand, within IAI47_21270 at 510.984 kb on - strand, within IAI47_21270 at 511.004 kb on - strand, within IAI47_21270 at 511.049 kb on + strand, within IAI47_21270 at 511.049 kb on + strand, within IAI47_21270 at 511.100 kb on + strand at 511.100 kb on + strand at 511.100 kb on + strand at 511.100 kb on + strand at 511.101 kb on - strand at 511.146 kb on + strand at 511.146 kb on + strand at 511.146 kb on + strand at 511.147 kb on - strand at 511.174 kb on + strand at 511.175 kb on - strand at 511.250 kb on + strand, within IAI47_21275 at 511.258 kb on + strand, within IAI47_21275 at 511.259 kb on - strand, within IAI47_21275 at 511.259 kb on - strand, within IAI47_21275 at 511.279 kb on + strand, within IAI47_21275 at 511.280 kb on - strand, within IAI47_21275 at 511.280 kb on - strand, within IAI47_21275 at 511.376 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.510 kb on + strand, within IAI47_21275 at 511.511 kb on - strand, within IAI47_21275 at 511.541 kb on - strand, within IAI47_21275 at 511.563 kb on - strand, within IAI47_21275 at 511.657 kb on + strand, within IAI47_21275 at 511.666 kb on + strand, within IAI47_21275 at 511.667 kb on - strand, within IAI47_21275 at 511.708 kb on + strand, within IAI47_21275 at 511.709 kb on - strand, within IAI47_21275 at 511.709 kb on - strand, within IAI47_21275 at 511.765 kb on - strand, within IAI47_21275 at 511.806 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.818 kb on - strand, within IAI47_21275 at 511.873 kb on - strand at 511.873 kb on - strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.887 kb on - strand at 511.887 kb on - strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 512.084 kb on + strand, within IAI47_21280
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.0; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 509,484 + IAI47_21260 0.88 +1.6 509,511 + -0.2 509,511 + -0.0 509,512 - +0.7 509,562 + +0.9 509,563 - -0.5 509,573 + +0.2 509,574 - +0.2 509,581 + -0.3 509,581 + +2.1 509,582 - +0.6 509,582 - +0.4 509,582 - +0.1 509,651 + +0.6 509,712 + +0.6 509,712 + +1.6 509,869 - IAI47_21265 0.24 +1.1 509,877 + IAI47_21265 0.25 +0.3 509,877 + IAI47_21265 0.25 +1.0 509,877 + IAI47_21265 0.25 -0.5 509,877 + IAI47_21265 0.25 +0.2 509,877 + IAI47_21265 0.25 -0.9 509,877 + IAI47_21265 0.25 +0.6 509,877 + IAI47_21265 0.25 +1.2 509,877 + IAI47_21265 0.25 +0.4 509,878 - IAI47_21265 0.25 -0.7 509,878 - IAI47_21265 0.25 -1.9 509,878 - IAI47_21265 0.25 -2.2 509,878 - IAI47_21265 0.25 -0.1 509,878 - IAI47_21265 0.25 -0.4 509,898 + IAI47_21265 0.27 -1.8 509,898 + IAI47_21265 0.27 +0.4 509,899 - IAI47_21265 0.28 -0.6 509,899 - IAI47_21265 0.28 +0.5 509,899 - IAI47_21265 0.28 +1.3 509,902 + IAI47_21265 0.28 +0.1 509,902 + IAI47_21265 0.28 +1.4 509,903 - IAI47_21265 0.28 -3.2 509,903 - IAI47_21265 0.28 -0.1 509,903 - IAI47_21265 0.28 -0.6 509,903 - IAI47_21265 0.28 +1.0 509,903 - IAI47_21265 0.28 +0.4 509,903 - IAI47_21265 0.28 -3.1 509,903 - IAI47_21265 0.28 +0.3 509,903 - IAI47_21265 0.28 -1.8 509,903 - IAI47_21265 0.28 +1.7 509,911 + IAI47_21265 0.29 +1.4 509,912 - IAI47_21265 0.29 -0.4 509,914 + IAI47_21265 0.29 -0.7 509,914 + IAI47_21265 0.29 -0.2 509,915 - IAI47_21265 0.30 +1.9 509,915 - IAI47_21265 0.30 +1.0 509,915 - IAI47_21265 0.30 -0.0 509,915 - IAI47_21265 0.30 -1.1 510,028 + IAI47_21265 0.44 +1.1 510,059 + IAI47_21265 0.48 -0.5 510,059 + IAI47_21265 0.48 -0.6 510,073 - IAI47_21265 0.49 +0.8 510,108 + IAI47_21265 0.54 +0.2 510,109 - IAI47_21265 0.54 +1.4 510,265 - IAI47_21265 0.73 +1.4 510,279 + IAI47_21265 0.75 -0.9 510,280 - IAI47_21265 0.75 -0.2 510,331 - IAI47_21265 0.81 -2.1 510,345 + IAI47_21265 0.83 -1.9 510,345 + IAI47_21265 0.83 +0.1 510,346 - IAI47_21265 0.83 -1.8 510,346 - IAI47_21265 0.83 +1.1 510,437 + -0.3 510,437 + -0.6 510,438 - +0.4 510,438 - -0.7 510,468 + -0.6 510,468 + -1.2 510,468 + -1.8 510,468 + +1.8 510,468 + +0.4 510,469 - -0.4 510,469 - +1.7 510,496 + -1.4 510,496 + -0.3 510,497 - -1.0 510,497 - -0.4 510,499 + +0.9 510,499 + -0.3 510,499 + -0.6 510,499 + +2.0 510,500 - +1.0 510,507 + -0.6 510,536 + +0.5 510,536 + -1.2 510,548 + IAI47_21270 0.10 -0.9 510,548 + IAI47_21270 0.10 -1.4 510,548 + IAI47_21270 0.10 +1.3 510,548 + IAI47_21270 0.10 +1.8 510,548 + IAI47_21270 0.10 -0.8 510,549 - IAI47_21270 0.10 +0.2 510,549 - IAI47_21270 0.10 +0.4 510,549 - IAI47_21270 0.10 -0.7 510,549 - IAI47_21270 0.10 -0.9 510,549 - IAI47_21270 0.10 +1.4 510,556 - IAI47_21270 0.11 -2.2 510,562 + IAI47_21270 0.12 -1.2 510,586 - IAI47_21270 0.16 -0.0 510,586 - IAI47_21270 0.16 +0.8 510,703 + IAI47_21270 0.33 +0.9 510,704 - IAI47_21270 0.33 -0.2 510,712 - IAI47_21270 0.34 +0.1 510,761 - IAI47_21270 0.41 +0.1 510,844 + IAI47_21270 0.53 -0.1 510,844 + IAI47_21270 0.53 +1.0 510,844 + IAI47_21270 0.53 +1.5 510,845 - IAI47_21270 0.54 -0.7 510,845 - IAI47_21270 0.54 -0.1 510,857 + IAI47_21270 0.55 +0.4 510,887 - IAI47_21270 0.60 -2.1 510,897 + IAI47_21270 0.61 +0.9 510,897 + IAI47_21270 0.61 +0.7 510,897 + IAI47_21270 0.61 +0.6 510,897 + IAI47_21270 0.61 -1.9 510,897 + IAI47_21270 0.61 +0.7 510,898 - IAI47_21270 0.61 -0.5 510,898 - IAI47_21270 0.61 -2.6 510,918 - IAI47_21270 0.64 -3.1 510,918 - IAI47_21270 0.64 -1.2 510,918 - IAI47_21270 0.64 -1.6 510,952 + IAI47_21270 0.69 +0.4 510,952 + IAI47_21270 0.69 -0.2 510,952 + IAI47_21270 0.69 +1.8 510,953 - IAI47_21270 0.69 -0.2 510,959 + IAI47_21270 0.70 -1.7 510,959 + IAI47_21270 0.70 +1.8 510,984 - IAI47_21270 0.74 -0.6 511,004 - IAI47_21270 0.77 +1.1 511,049 + IAI47_21270 0.83 -1.8 511,049 + IAI47_21270 0.83 -1.1 511,100 + -0.7 511,100 + +0.9 511,100 + +1.0 511,100 + +0.6 511,101 - -0.4 511,146 + +1.4 511,146 + +0.1 511,146 + +1.4 511,147 - -1.4 511,174 + -1.3 511,175 - -0.6 511,250 + IAI47_21275 0.11 -0.2 511,258 + IAI47_21275 0.12 -2.2 511,259 - IAI47_21275 0.12 -0.7 511,259 - IAI47_21275 0.12 -2.1 511,279 + IAI47_21275 0.15 -0.4 511,280 - IAI47_21275 0.15 +0.2 511,280 - IAI47_21275 0.15 +0.4 511,376 + IAI47_21275 0.28 +0.3 511,436 + IAI47_21275 0.36 -0.1 511,436 + IAI47_21275 0.36 +0.4 511,436 + IAI47_21275 0.36 -1.9 511,437 - IAI47_21275 0.36 -0.3 511,437 - IAI47_21275 0.36 -2.1 511,437 - IAI47_21275 0.36 -2.4 511,510 + IAI47_21275 0.46 +0.1 511,511 - IAI47_21275 0.47 -0.9 511,541 - IAI47_21275 0.51 +0.4 511,563 - IAI47_21275 0.54 -0.9 511,657 + IAI47_21275 0.67 +2.0 511,666 + IAI47_21275 0.68 -0.4 511,667 - IAI47_21275 0.68 -2.3 511,708 + IAI47_21275 0.74 +0.0 511,709 - IAI47_21275 0.74 +0.5 511,709 - IAI47_21275 0.74 +0.3 511,765 - IAI47_21275 0.82 -0.1 511,806 + IAI47_21275 0.87 -2.5 511,817 + IAI47_21275 0.89 -0.6 511,817 + IAI47_21275 0.89 +1.0 511,818 - IAI47_21275 0.89 -1.8 511,873 - -1.4 511,873 - -0.3 511,886 + -2.3 511,886 + -0.5 511,886 + -2.4 511,886 + -0.6 511,887 - +1.0 511,887 - -0.9 511,906 + +2.3 511,906 + +0.4 511,906 + -0.7 511,906 + +1.4 512,084 + IAI47_21280 0.21 +0.4
Or see this region's nucleotide sequence