Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23825

Experiment: Trisodium citrate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23815 and BWI76_RS23820 are separated by 121 nucleotidesBWI76_RS23820 and BWI76_RS23825 are separated by 8 nucleotidesBWI76_RS23825 and BWI76_RS23830 overlap by 20 nucleotidesBWI76_RS23830 and BWI76_RS23835 are separated by 243 nucleotides BWI76_RS23815: BWI76_RS23815 - site-specific tyrosine recombinase XerD, at 4,971,772 to 4,972,668 _RS23815 BWI76_RS23820: BWI76_RS23820 - flavodoxin FldB, at 4,972,790 to 4,973,311 _RS23820 BWI76_RS23825: BWI76_RS23825 - hypothetical protein, at 4,973,320 to 4,973,730 _RS23825 BWI76_RS23830: BWI76_RS23830 - hypothetical protein, at 4,973,711 to 4,973,977 _RS23830 BWI76_RS23835: BWI76_RS23835 - folate-binding protein, at 4,974,221 to 4,975,204 _RS23835 Position (kb) 4973 4974Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4972.742 kb on + strandat 4972.866 kb on + strand, within BWI76_RS23820at 4973.138 kb on + strand, within BWI76_RS23820at 4973.139 kb on + strand, within BWI76_RS23820at 4973.508 kb on + strand, within BWI76_RS23825at 4973.516 kb on - strand, within BWI76_RS23825at 4973.573 kb on + strand, within BWI76_RS23825at 4973.581 kb on - strand, within BWI76_RS23825at 4973.602 kb on + strand, within BWI76_RS23825at 4973.606 kb on - strand, within BWI76_RS23825at 4973.613 kb on + strand, within BWI76_RS23825at 4973.623 kb on - strand, within BWI76_RS23825at 4973.635 kb on - strand, within BWI76_RS23825at 4973.773 kb on + strand, within BWI76_RS23830at 4973.773 kb on + strand, within BWI76_RS23830at 4973.868 kb on + strand, within BWI76_RS23830at 4973.879 kb on + strand, within BWI76_RS23830at 4973.931 kb on + strand, within BWI76_RS23830at 4973.938 kb on + strand, within BWI76_RS23830at 4974.039 kb on - strandat 4974.121 kb on + strandat 4974.129 kb on - strandat 4974.129 kb on - strandat 4974.206 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trisodium citrate (C)
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4,972,742 + +0.3
4,972,866 + BWI76_RS23820 0.15 -0.3
4,973,138 + BWI76_RS23820 0.67 +0.5
4,973,139 + BWI76_RS23820 0.67 -0.6
4,973,508 + BWI76_RS23825 0.46 +0.2
4,973,516 - BWI76_RS23825 0.48 +0.2
4,973,573 + BWI76_RS23825 0.62 -0.2
4,973,581 - BWI76_RS23825 0.64 +0.2
4,973,602 + BWI76_RS23825 0.69 +0.9
4,973,606 - BWI76_RS23825 0.70 +0.2
4,973,613 + BWI76_RS23825 0.71 -0.5
4,973,623 - BWI76_RS23825 0.74 +1.1
4,973,635 - BWI76_RS23825 0.77 -0.6
4,973,773 + BWI76_RS23830 0.23 -4.2
4,973,773 + BWI76_RS23830 0.23 -3.2
4,973,868 + BWI76_RS23830 0.59 -1.1
4,973,879 + BWI76_RS23830 0.63 -2.9
4,973,931 + BWI76_RS23830 0.82 -3.8
4,973,938 + BWI76_RS23830 0.85 -4.0
4,974,039 - -0.1
4,974,121 + -0.5
4,974,129 - -0.0
4,974,129 - +0.5
4,974,206 + +0.4

Or see this region's nucleotide sequence