Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04550 and BWI76_RS04555 are separated by 323 nucleotides BWI76_RS04555 and BWI76_RS04560 are separated by 168 nucleotides BWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotides
BWI76_RS04550: BWI76_RS04550 - two-component sensor kinase, at 942,017 to 943,642
_RS04550
BWI76_RS04555: BWI76_RS04555 - citrate/acetate antiporter, at 943,966 to 945,321
_RS04555
BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029
_RS04560
BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770
_RS04565
Position (kb)
943
944
945
946 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 943.022 kb on - strand, within BWI76_RS04550 at 943.082 kb on + strand, within BWI76_RS04550 at 943.243 kb on + strand, within BWI76_RS04550 at 943.280 kb on + strand, within BWI76_RS04550 at 943.331 kb on + strand, within BWI76_RS04550 at 943.336 kb on + strand, within BWI76_RS04550 at 943.360 kb on + strand, within BWI76_RS04550 at 943.406 kb on - strand, within BWI76_RS04550 at 943.455 kb on + strand, within BWI76_RS04550 at 943.537 kb on + strand at 943.537 kb on + strand at 943.537 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.567 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.618 kb on + strand at 943.625 kb on - strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.703 kb on + strand at 943.703 kb on + strand at 943.708 kb on + strand at 943.710 kb on + strand at 943.713 kb on + strand at 943.716 kb on - strand at 943.718 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.734 kb on + strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.748 kb on + strand at 943.774 kb on - strand at 943.776 kb on + strand at 943.823 kb on + strand at 943.862 kb on - strand at 943.881 kb on - strand at 943.902 kb on + strand at 943.905 kb on + strand at 943.905 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.925 kb on - strand at 943.925 kb on - strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.934 kb on - strand at 943.934 kb on - strand at 943.959 kb on - strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.988 kb on - strand at 943.990 kb on - strand at 944.042 kb on + strand at 944.060 kb on + strand at 944.072 kb on + strand at 944.180 kb on + strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.250 kb on - strand, within BWI76_RS04555 at 944.267 kb on + strand, within BWI76_RS04555 at 944.288 kb on + strand, within BWI76_RS04555 at 944.390 kb on + strand, within BWI76_RS04555 at 944.399 kb on + strand, within BWI76_RS04555 at 944.409 kb on + strand, within BWI76_RS04555 at 944.474 kb on + strand, within BWI76_RS04555 at 944.665 kb on - strand, within BWI76_RS04555 at 944.872 kb on - strand, within BWI76_RS04555 at 944.905 kb on + strand, within BWI76_RS04555 at 944.910 kb on + strand, within BWI76_RS04555 at 944.910 kb on + strand, within BWI76_RS04555 at 944.919 kb on + strand, within BWI76_RS04555 at 944.970 kb on - strand, within BWI76_RS04555 at 944.983 kb on + strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 944.991 kb on - strand, within BWI76_RS04555 at 945.058 kb on - strand, within BWI76_RS04555 at 945.058 kb on - strand, within BWI76_RS04555 at 945.072 kb on + strand, within BWI76_RS04555 at 945.187 kb on - strand at 945.525 kb on - strand at 945.626 kb on - strand, within BWI76_RS04560 at 945.644 kb on + strand, within BWI76_RS04560 at 945.773 kb on - strand, within BWI76_RS04560 at 945.809 kb on - strand, within BWI76_RS04560 at 946.053 kb on - strand at 946.098 kb on - strand at 946.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 943,022 - BWI76_RS04550 0.62 -0.9 943,082 + BWI76_RS04550 0.65 +0.6 943,243 + BWI76_RS04550 0.75 -1.3 943,280 + BWI76_RS04550 0.78 -0.8 943,331 + BWI76_RS04550 0.81 -1.5 943,336 + BWI76_RS04550 0.81 -2.5 943,360 + BWI76_RS04550 0.83 -0.5 943,406 - BWI76_RS04550 0.85 -0.7 943,455 + BWI76_RS04550 0.88 -3.6 943,537 + -2.2 943,537 + -1.4 943,537 + +0.2 943,566 + -1.7 943,566 + -2.1 943,566 + -2.2 943,566 + -2.0 943,566 + -2.7 943,567 - -1.0 943,574 - +0.6 943,574 - -1.4 943,574 - -1.2 943,574 - -0.6 943,574 - -1.1 943,618 + -2.0 943,625 - -0.7 943,645 + -1.3 943,645 + -1.3 943,645 + -1.9 943,645 + -0.1 943,645 + -0.9 943,645 + -1.9 943,653 - +0.7 943,653 - -0.9 943,653 - +0.3 943,653 - +0.1 943,653 - +0.3 943,657 + -1.1 943,657 + -3.3 943,657 + -1.6 943,657 + -1.1 943,657 + -2.5 943,657 + -0.6 943,665 - +0.0 943,665 - -0.1 943,665 - +0.9 943,665 - -0.2 943,665 - -0.3 943,703 + -1.9 943,703 + +0.6 943,708 + -1.6 943,710 + -1.3 943,713 + -0.9 943,716 - +0.3 943,718 + -1.3 943,733 + -0.7 943,733 + -3.1 943,733 + +0.6 943,734 + -1.5 943,741 - +0.1 943,741 - +0.6 943,741 - +0.6 943,748 + +0.4 943,774 - -0.1 943,776 + -0.2 943,823 + -0.7 943,862 - -0.8 943,881 - -1.0 943,902 + -2.3 943,905 + -2.2 943,905 + -2.9 943,907 + -1.0 943,907 + -2.0 943,907 + -1.6 943,907 + -1.3 943,907 + -1.4 943,907 + -1.2 943,915 - -2.3 943,915 - -2.7 943,915 - -0.0 943,915 - -2.9 943,915 - -1.7 943,915 - -1.3 943,915 - -2.5 943,917 + -1.2 943,917 + -0.3 943,917 + -1.8 943,925 - -0.3 943,925 - -0.9 943,926 + -2.8 943,926 + +0.9 943,926 + -1.7 943,934 - -1.2 943,934 - -0.4 943,959 - -2.0 943,973 + -1.2 943,973 + -0.9 943,973 + -0.6 943,988 - -2.0 943,990 - -0.9 944,042 + -0.5 944,060 + -1.3 944,072 + -2.2 944,180 + BWI76_RS04555 0.16 -1.4 944,240 - BWI76_RS04555 0.20 -1.8 944,240 - BWI76_RS04555 0.20 -2.4 944,250 - BWI76_RS04555 0.21 -1.2 944,267 + BWI76_RS04555 0.22 -1.1 944,288 + BWI76_RS04555 0.24 -0.7 944,390 + BWI76_RS04555 0.31 -2.1 944,399 + BWI76_RS04555 0.32 -2.0 944,409 + BWI76_RS04555 0.33 -2.2 944,474 + BWI76_RS04555 0.37 -2.0 944,665 - BWI76_RS04555 0.52 -2.2 944,872 - BWI76_RS04555 0.67 -2.1 944,905 + BWI76_RS04555 0.69 -0.7 944,910 + BWI76_RS04555 0.70 -0.6 944,910 + BWI76_RS04555 0.70 -1.5 944,919 + BWI76_RS04555 0.70 -1.2 944,970 - BWI76_RS04555 0.74 -1.0 944,983 + BWI76_RS04555 0.75 -1.4 944,991 - BWI76_RS04555 0.76 -0.7 944,991 - BWI76_RS04555 0.76 -0.7 944,991 - BWI76_RS04555 0.76 -1.5 945,058 - BWI76_RS04555 0.81 -2.5 945,058 - BWI76_RS04555 0.81 -1.1 945,072 + BWI76_RS04555 0.82 -1.3 945,187 - -1.6 945,525 - +0.6 945,626 - BWI76_RS04560 0.25 -0.1 945,644 + BWI76_RS04560 0.29 +1.0 945,773 - BWI76_RS04560 0.52 -0.7 945,809 - BWI76_RS04560 0.59 +1.3 946,053 - +1.3 946,098 - -0.1 946,108 - -1.5
Or see this region's nucleotide sequence