Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25380 and AO353_25385 are separated by 46 nucleotides AO353_25385 and AO353_25390 overlap by 4 nucleotides AO353_25390 and AO353_25395 are separated by 3 nucleotides AO353_25395 and AO353_25400 are separated by 43 nucleotides
AO353_25380: AO353_25380 - bestrophin, at 5,598,338 to 5,599,237
_25380
AO353_25385: AO353_25385 - molybdenum cofactor biosynthesis protein MoaC, at 5,599,284 to 5,599,790
_25385
AO353_25390: AO353_25390 - molybdenum cofactor biosynthesis protein MoaD, at 5,599,787 to 5,600,038
_25390
AO353_25395: AO353_25395 - molybdenum cofactor biosynthesis protein MoaE, at 5,600,042 to 5,600,506
_25395
AO353_25400: AO353_25400 - molybdenum cofactor biosynthesis protein MoaA, at 5,600,550 to 5,601,755
_25400
Position (kb) 5599
5600
5601 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5598.980 kb on + strand, within AO353_25380 at 5598.981 kb on - strand, within AO353_25380 at 5598.981 kb on - strand, within AO353_25380 at 5598.984 kb on + strand, within AO353_25380 at 5598.985 kb on - strand, within AO353_25380 at 5598.985 kb on - strand, within AO353_25380 at 5599.062 kb on - strand, within AO353_25380 at 5599.062 kb on - strand, within AO353_25380 at 5599.062 kb on - strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.063 kb on + strand, within AO353_25380 at 5599.064 kb on - strand, within AO353_25380 at 5599.064 kb on - strand, within AO353_25380 at 5599.064 kb on - strand, within AO353_25380 at 5599.064 kb on - strand, within AO353_25380 at 5599.280 kb on - strand at 5599.280 kb on - strand at 5599.437 kb on - strand, within AO353_25385 at 5599.484 kb on + strand, within AO353_25385 at 5599.683 kb on + strand, within AO353_25385 at 5599.683 kb on + strand, within AO353_25385 at 5599.684 kb on - strand, within AO353_25385 at 5599.684 kb on - strand, within AO353_25385 at 5599.802 kb on + strand at 5599.802 kb on + strand at 5599.802 kb on + strand at 5599.802 kb on + strand at 5599.802 kb on + strand at 5599.802 kb on + strand at 5599.803 kb on - strand at 5599.803 kb on - strand at 5599.972 kb on + strand, within AO353_25390 at 5599.973 kb on - strand, within AO353_25390 at 5600.067 kb on + strand at 5600.068 kb on - strand at 5600.068 kb on - strand at 5600.068 kb on - strand at 5600.384 kb on + strand, within AO353_25395 at 5600.384 kb on + strand, within AO353_25395 at 5600.384 kb on + strand, within AO353_25395 at 5600.526 kb on + strand at 5600.526 kb on + strand at 5600.527 kb on - strand at 5600.542 kb on + strand at 5600.542 kb on + strand at 5600.543 kb on - strand at 5600.543 kb on - strand at 5600.543 kb on - strand at 5600.552 kb on - strand at 5600.552 kb on - strand at 5600.574 kb on - strand at 5600.716 kb on + strand, within AO353_25400 at 5600.717 kb on - strand, within AO353_25400 at 5600.717 kb on - strand, within AO353_25400 at 5600.717 kb on - strand, within AO353_25400 at 5600.717 kb on - strand, within AO353_25400 at 5600.812 kb on + strand, within AO353_25400 at 5600.813 kb on - strand, within AO353_25400
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,598,980 + AO353_25380 0.71 +0.1 5,598,981 - AO353_25380 0.71 -0.2 5,598,981 - AO353_25380 0.71 +2.6 5,598,984 + AO353_25380 0.72 +1.0 5,598,985 - AO353_25380 0.72 +0.1 5,598,985 - AO353_25380 0.72 -0.8 5,599,062 - AO353_25380 0.80 +0.6 5,599,062 - AO353_25380 0.80 +2.8 5,599,062 - AO353_25380 0.80 +1.2 5,599,063 + AO353_25380 0.81 +1.6 5,599,063 + AO353_25380 0.81 -0.5 5,599,063 + AO353_25380 0.81 +0.6 5,599,063 + AO353_25380 0.81 -3.9 5,599,063 + AO353_25380 0.81 -1.8 5,599,063 + AO353_25380 0.81 +0.4 5,599,064 - AO353_25380 0.81 -1.5 5,599,064 - AO353_25380 0.81 -0.8 5,599,064 - AO353_25380 0.81 +2.2 5,599,064 - AO353_25380 0.81 -0.8 5,599,280 - -0.5 5,599,280 - +1.0 5,599,437 - AO353_25385 0.30 -2.8 5,599,484 + AO353_25385 0.39 +1.1 5,599,683 + AO353_25385 0.79 +1.0 5,599,683 + AO353_25385 0.79 -1.1 5,599,684 - AO353_25385 0.79 -0.3 5,599,684 - AO353_25385 0.79 +0.4 5,599,802 + +1.0 5,599,802 + +1.0 5,599,802 + +3.4 5,599,802 + -1.7 5,599,802 + -1.8 5,599,802 + -1.6 5,599,803 - +2.7 5,599,803 - +0.4 5,599,972 + AO353_25390 0.73 +2.1 5,599,973 - AO353_25390 0.74 +0.0 5,600,067 + -1.6 5,600,068 - -1.4 5,600,068 - +1.4 5,600,068 - +1.2 5,600,384 + AO353_25395 0.74 -2.1 5,600,384 + AO353_25395 0.74 -0.6 5,600,384 + AO353_25395 0.74 +3.1 5,600,526 + +0.2 5,600,526 + -1.1 5,600,527 - +1.0 5,600,542 + +1.3 5,600,542 + +0.6 5,600,543 - -1.0 5,600,543 - -1.0 5,600,543 - -2.0 5,600,552 - +1.9 5,600,552 - -0.4 5,600,574 - -2.3 5,600,716 + AO353_25400 0.14 -1.4 5,600,717 - AO353_25400 0.14 +0.7 5,600,717 - AO353_25400 0.14 +2.1 5,600,717 - AO353_25400 0.14 +1.3 5,600,717 - AO353_25400 0.14 -2.8 5,600,812 + AO353_25400 0.22 +0.7 5,600,813 - AO353_25400 0.22 -3.4
Or see this region's nucleotide sequence