Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_24590 and AO353_24595 are separated by 320 nucleotides AO353_24595 and AO353_24600 are separated by 206 nucleotides AO353_24600 and AO353_24605 are separated by 223 nucleotides
AO353_24590: AO353_24590 - hypothetical protein, at 5,409,014 to 5,410,156
_24590
AO353_24595: AO353_24595 - hypothetical protein, at 5,410,477 to 5,410,725
_24595
AO353_24600: AO353_24600 - hypothetical protein, at 5,410,932 to 5,411,279
_24600
AO353_24605: AO353_24605 - hypothetical protein, at 5,411,503 to 5,412,609
_24605
Position (kb) 5410
5411 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5409.520 kb on - strand, within AO353_24590 at 5409.547 kb on + strand, within AO353_24590 at 5409.547 kb on + strand, within AO353_24590 at 5409.547 kb on + strand, within AO353_24590 at 5409.547 kb on + strand at 5409.577 kb on + strand, within AO353_24590 at 5409.577 kb on + strand, within AO353_24590 at 5409.577 kb on + strand, within AO353_24590 at 5409.577 kb on + strand, within AO353_24590 at 5409.594 kb on + strand, within AO353_24590 at 5409.594 kb on + strand, within AO353_24590 at 5409.594 kb on + strand, within AO353_24590 at 5409.883 kb on + strand at 5409.883 kb on + strand, within AO353_24590 at 5409.883 kb on + strand, within AO353_24590 at 5409.884 kb on - strand, within AO353_24590 at 5409.903 kb on + strand, within AO353_24590 at 5409.903 kb on + strand, within AO353_24590 at 5409.903 kb on + strand, within AO353_24590 at 5409.904 kb on - strand, within AO353_24590 at 5409.904 kb on - strand, within AO353_24590 at 5409.904 kb on - strand, within AO353_24590 at 5409.986 kb on + strand, within AO353_24590 at 5409.986 kb on + strand, within AO353_24590 at 5409.986 kb on + strand, within AO353_24590 at 5409.987 kb on - strand, within AO353_24590 at 5410.148 kb on + strand at 5410.161 kb on + strand at 5410.162 kb on - strand at 5410.162 kb on - strand at 5410.242 kb on + strand at 5410.242 kb on + strand at 5410.242 kb on + strand at 5410.242 kb on + strand at 5410.243 kb on - strand at 5410.243 kb on - strand at 5410.243 kb on - strand at 5410.282 kb on - strand at 5410.390 kb on + strand at 5410.390 kb on + strand at 5410.420 kb on + strand at 5410.421 kb on - strand at 5410.442 kb on - strand at 5410.442 kb on - strand at 5410.445 kb on + strand at 5410.552 kb on - strand, within AO353_24595 at 5410.565 kb on + strand, within AO353_24595 at 5410.566 kb on - strand, within AO353_24595 at 5410.667 kb on - strand, within AO353_24595 at 5410.707 kb on + strand at 5410.711 kb on - strand at 5410.711 kb on - strand at 5410.789 kb on + strand at 5410.806 kb on + strand at 5410.811 kb on - strand at 5410.819 kb on + strand at 5411.027 kb on + strand, within AO353_24600 at 5411.103 kb on - strand, within AO353_24600 at 5411.117 kb on + strand, within AO353_24600 at 5411.159 kb on + strand, within AO353_24600 at 5411.169 kb on + strand, within AO353_24600 at 5411.169 kb on + strand, within AO353_24600 at 5411.203 kb on + strand, within AO353_24600 at 5411.232 kb on + strand, within AO353_24600 at 5411.233 kb on - strand, within AO353_24600 at 5411.355 kb on + strand at 5411.355 kb on + strand at 5411.365 kb on + strand at 5411.439 kb on + strand at 5411.440 kb on - strand at 5411.466 kb on - strand at 5411.466 kb on - strand at 5411.527 kb on + strand at 5411.527 kb on + strand at 5411.527 kb on + strand at 5411.528 kb on - strand at 5411.531 kb on + strand at 5411.532 kb on - strand at 5411.532 kb on - strand at 5411.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,409,520 - AO353_24590 0.44 -1.7 5,409,547 + AO353_24590 0.47 -1.2 5,409,547 + AO353_24590 0.47 -2.2 5,409,547 + AO353_24590 0.47 +1.0 5,409,547 + +0.3 5,409,577 + AO353_24590 0.49 -1.9 5,409,577 + AO353_24590 0.49 +2.8 5,409,577 + AO353_24590 0.49 +1.6 5,409,577 + AO353_24590 0.49 +2.5 5,409,594 + AO353_24590 0.51 +0.4 5,409,594 + AO353_24590 0.51 +0.6 5,409,594 + AO353_24590 0.51 +0.5 5,409,883 + +0.3 5,409,883 + AO353_24590 0.76 +0.2 5,409,883 + AO353_24590 0.76 -0.9 5,409,884 - AO353_24590 0.76 +2.1 5,409,903 + AO353_24590 0.78 -0.4 5,409,903 + AO353_24590 0.78 -2.4 5,409,903 + AO353_24590 0.78 -1.5 5,409,904 - AO353_24590 0.78 +0.9 5,409,904 - AO353_24590 0.78 -0.9 5,409,904 - AO353_24590 0.78 +0.8 5,409,986 + AO353_24590 0.85 -1.2 5,409,986 + AO353_24590 0.85 +1.2 5,409,986 + AO353_24590 0.85 -1.1 5,409,987 - AO353_24590 0.85 -1.9 5,410,148 + -1.6 5,410,161 + +1.0 5,410,162 - -1.3 5,410,162 - -1.0 5,410,242 + +2.8 5,410,242 + +0.6 5,410,242 + -1.3 5,410,242 + -0.9 5,410,243 - +0.6 5,410,243 - +0.1 5,410,243 - -2.2 5,410,282 - -1.6 5,410,390 + +1.7 5,410,390 + +0.8 5,410,420 + +0.1 5,410,421 - -2.2 5,410,442 - +2.3 5,410,442 - +0.0 5,410,445 + -1.9 5,410,552 - AO353_24595 0.30 -0.4 5,410,565 + AO353_24595 0.35 -0.2 5,410,566 - AO353_24595 0.36 -2.8 5,410,667 - AO353_24595 0.76 -1.6 5,410,707 + +2.1 5,410,711 - +1.9 5,410,711 - -1.2 5,410,789 + +0.9 5,410,806 + -2.8 5,410,811 - +1.9 5,410,819 + -0.4 5,411,027 + AO353_24600 0.27 -1.0 5,411,103 - AO353_24600 0.49 +0.7 5,411,117 + AO353_24600 0.53 -0.6 5,411,159 + AO353_24600 0.65 +0.1 5,411,169 + AO353_24600 0.68 -1.4 5,411,169 + AO353_24600 0.68 -3.1 5,411,203 + AO353_24600 0.78 -1.3 5,411,232 + AO353_24600 0.86 +2.0 5,411,233 - AO353_24600 0.86 +0.5 5,411,355 + +1.3 5,411,355 + +0.6 5,411,365 + -1.8 5,411,439 + -1.0 5,411,440 - -1.8 5,411,466 - +0.3 5,411,466 - -0.7 5,411,527 + -2.5 5,411,527 + -1.3 5,411,527 + -2.4 5,411,528 - -2.7 5,411,531 + -0.9 5,411,532 - +2.9 5,411,532 - -3.1 5,411,594 - +0.3
Or see this region's nucleotide sequence