Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23210 and AO353_23215 are separated by 187 nucleotides AO353_23215 and AO353_23220 are separated by 40 nucleotides AO353_23220 and AO353_23225 are separated by 235 nucleotides
AO353_23210: AO353_23210 - hydroperoxidase, at 5,091,946 to 5,094,153
_23210
AO353_23215: AO353_23215 - calpastatin, at 5,094,341 to 5,094,766
_23215
AO353_23220: AO353_23220 - hypothetical protein, at 5,094,807 to 5,095,106
_23220
AO353_23225: AO353_23225 - alpha/beta hydrolase, at 5,095,342 to 5,096,409
_23225
Position (kb)
5094
5095
5096 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5094.030 kb on + strand at 5094.030 kb on + strand at 5094.061 kb on + strand at 5094.061 kb on + strand at 5094.061 kb on + strand at 5094.061 kb on + strand at 5094.061 kb on + strand at 5094.062 kb on - strand at 5094.070 kb on - strand at 5094.151 kb on + strand at 5094.253 kb on + strand at 5094.253 kb on + strand at 5094.290 kb on - strand at 5094.326 kb on + strand at 5094.326 kb on + strand at 5094.326 kb on + strand at 5094.342 kb on + strand at 5094.343 kb on - strand at 5094.343 kb on - strand at 5094.434 kb on - strand, within AO353_23215 at 5094.443 kb on + strand at 5094.443 kb on + strand, within AO353_23215 at 5094.444 kb on - strand, within AO353_23215 at 5094.573 kb on + strand, within AO353_23215 at 5094.573 kb on + strand, within AO353_23215 at 5094.574 kb on - strand, within AO353_23215 at 5094.574 kb on - strand, within AO353_23215 at 5094.772 kb on + strand at 5094.773 kb on - strand at 5094.773 kb on - strand at 5094.773 kb on - strand at 5094.808 kb on + strand at 5094.872 kb on - strand, within AO353_23220 at 5094.885 kb on - strand, within AO353_23220 at 5094.954 kb on + strand, within AO353_23220 at 5094.968 kb on - strand, within AO353_23220 at 5094.998 kb on - strand, within AO353_23220 at 5095.082 kb on - strand at 5095.120 kb on + strand at 5095.120 kb on + strand at 5095.120 kb on + strand at 5095.142 kb on + strand at 5095.142 kb on + strand at 5095.143 kb on - strand at 5095.152 kb on - strand at 5095.152 kb on - strand at 5095.281 kb on + strand at 5095.281 kb on + strand at 5095.310 kb on - strand at 5095.439 kb on + strand at 5095.439 kb on + strand at 5095.443 kb on + strand at 5095.464 kb on - strand, within AO353_23225 at 5095.464 kb on - strand, within AO353_23225 at 5095.464 kb on - strand, within AO353_23225 at 5095.558 kb on + strand, within AO353_23225 at 5095.558 kb on + strand, within AO353_23225 at 5095.569 kb on - strand, within AO353_23225 at 5095.569 kb on - strand, within AO353_23225 at 5095.607 kb on + strand, within AO353_23225 at 5095.607 kb on + strand, within AO353_23225 at 5095.668 kb on - strand, within AO353_23225 at 5095.668 kb on - strand, within AO353_23225 at 5095.711 kb on + strand, within AO353_23225 at 5095.775 kb on + strand, within AO353_23225 at 5095.776 kb on - strand, within AO353_23225 at 5095.851 kb on - strand, within AO353_23225 at 5095.931 kb on + strand, within AO353_23225 at 5095.932 kb on - strand, within AO353_23225 at 5096.063 kb on + strand, within AO353_23225 at 5096.098 kb on + strand, within AO353_23225 at 5096.098 kb on + strand, within AO353_23225 at 5096.098 kb on + strand, within AO353_23225 at 5096.099 kb on - strand, within AO353_23225 at 5096.099 kb on - strand, within AO353_23225 at 5096.099 kb on - strand, within AO353_23225
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,094,030 + -0.5 5,094,030 + -1.0 5,094,061 + -1.1 5,094,061 + -1.3 5,094,061 + -2.9 5,094,061 + +0.2 5,094,061 + +0.7 5,094,062 - -0.1 5,094,070 - -1.5 5,094,151 + -0.9 5,094,253 + +2.5 5,094,253 + +0.0 5,094,290 - +2.0 5,094,326 + -1.3 5,094,326 + -0.8 5,094,326 + -1.0 5,094,342 + -0.4 5,094,343 - -1.3 5,094,343 - +0.4 5,094,434 - AO353_23215 0.22 -0.2 5,094,443 + -0.3 5,094,443 + AO353_23215 0.24 +1.6 5,094,444 - AO353_23215 0.24 +2.2 5,094,573 + AO353_23215 0.54 -2.2 5,094,573 + AO353_23215 0.54 -1.8 5,094,574 - AO353_23215 0.55 -1.5 5,094,574 - AO353_23215 0.55 -0.1 5,094,772 + +0.8 5,094,773 - +2.4 5,094,773 - -2.5 5,094,773 - +1.5 5,094,808 + -1.1 5,094,872 - AO353_23220 0.22 -0.8 5,094,885 - AO353_23220 0.26 -1.1 5,094,954 + AO353_23220 0.49 +0.2 5,094,968 - AO353_23220 0.54 -1.0 5,094,998 - AO353_23220 0.64 -1.3 5,095,082 - -0.1 5,095,120 + -1.2 5,095,120 + -2.1 5,095,120 + -1.6 5,095,142 + +0.3 5,095,142 + +2.5 5,095,143 - -1.4 5,095,152 - +2.0 5,095,152 - +1.3 5,095,281 + -1.5 5,095,281 + +1.7 5,095,310 - -1.5 5,095,439 + +0.6 5,095,439 + -0.2 5,095,443 + -2.3 5,095,464 - AO353_23225 0.11 -0.2 5,095,464 - AO353_23225 0.11 -2.4 5,095,464 - AO353_23225 0.11 -2.0 5,095,558 + AO353_23225 0.20 -0.5 5,095,558 + AO353_23225 0.20 -2.0 5,095,569 - AO353_23225 0.21 +2.8 5,095,569 - AO353_23225 0.21 -0.7 5,095,607 + AO353_23225 0.25 +0.4 5,095,607 + AO353_23225 0.25 +2.3 5,095,668 - AO353_23225 0.31 +0.4 5,095,668 - AO353_23225 0.31 -0.1 5,095,711 + AO353_23225 0.35 -0.4 5,095,775 + AO353_23225 0.41 +0.3 5,095,776 - AO353_23225 0.41 -2.1 5,095,851 - AO353_23225 0.48 -0.8 5,095,931 + AO353_23225 0.55 -0.3 5,095,932 - AO353_23225 0.55 +0.5 5,096,063 + AO353_23225 0.68 +0.9 5,096,098 + AO353_23225 0.71 -1.8 5,096,098 + AO353_23225 0.71 +1.3 5,096,098 + AO353_23225 0.71 -2.5 5,096,099 - AO353_23225 0.71 +0.9 5,096,099 - AO353_23225 0.71 -1.6 5,096,099 - AO353_23225 0.71 -0.2
Or see this region's nucleotide sequence