Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12425 and AO353_12430 are separated by 57 nucleotides AO353_12430 and AO353_12435 are separated by 287 nucleotides AO353_12435 and AO353_12440 are separated by 1 nucleotides
AO353_12425: AO353_12425 - ATP-dependent protease ATP-binding subunit HslU, at 2,731,789 to 2,733,129
_12425
AO353_12430: AO353_12430 - ATP-dependent protease subunit HslV, at 2,733,187 to 2,733,717
_12430
AO353_12435: AO353_12435 - cell division protein, at 2,734,005 to 2,734,706
_12435
AO353_12440: AO353_12440 - arginine--tRNA ligase, at 2,734,708 to 2,736,444
_12440
Position (kb)
2734
2735 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2733.189 kb on - strand at 2733.189 kb on - strand at 2733.189 kb on - strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.820 kb on + strand at 2733.821 kb on - strand at 2733.821 kb on - strand at 2733.821 kb on - strand at 2733.821 kb on - strand at 2733.821 kb on - strand at 2733.876 kb on - strand at 2733.919 kb on + strand at 2733.920 kb on - strand at 2733.920 kb on - strand at 2734.027 kb on - strand at 2734.027 kb on - strand at 2734.027 kb on - strand at 2734.027 kb on - strand at 2734.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,733,189 - +1.2 2,733,189 - +0.2 2,733,189 - +4.7 2,733,820 + +0.9 2,733,820 + +2.1 2,733,820 + -2.0 2,733,820 + -0.7 2,733,820 + -1.4 2,733,820 + +3.0 2,733,820 + -0.6 2,733,821 - +1.7 2,733,821 - +0.6 2,733,821 - +1.9 2,733,821 - +0.6 2,733,821 - +0.5 2,733,876 - -0.7 2,733,919 + -1.4 2,733,920 - +0.8 2,733,920 - +2.2 2,734,027 - -0.9 2,734,027 - -0.6 2,734,027 - +0.1 2,734,027 - -1.6 2,734,710 - -1.7
Or see this region's nucleotide sequence