Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_06695 and AO353_06700 overlap by 4 nucleotides AO353_06700 and AO353_06705 are separated by 90 nucleotides AO353_06705 and AO353_06710 are separated by 31 nucleotides AO353_06710 and AO353_06715 are separated by 48 nucleotides
AO353_06695: AO353_06695 - antitermination protein NusB, at 1,491,754 to 1,492,254
_06695
AO353_06700: AO353_06700 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,492,251 to 1,492,727
_06700
AO353_06705: AO353_06705 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 1,492,818 to 1,493,909
_06705
AO353_06710: AO353_06710 - riboflavin synthase subunit alpha, at 1,493,941 to 1,494,603
_06710
AO353_06715: AO353_06715 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 1,494,652 to 1,495,785
_06715
Position (kb)
1492
1493
1494 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1492.742 kb on - strand at 1492.742 kb on - strand at 1492.781 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1492.835 kb on - strand at 1494.026 kb on - strand, within AO353_06710 at 1494.026 kb on - strand, within AO353_06710 at 1494.026 kb on - strand, within AO353_06710 at 1494.026 kb on - strand, within AO353_06710 at 1494.653 kb on + strand at 1494.653 kb on + strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.654 kb on - strand at 1494.655 kb on + strand at 1494.655 kb on + strand at 1494.655 kb on + strand at 1494.655 kb on + strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand at 1494.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,492,742 - -1.6 1,492,742 - -1.0 1,492,781 - +1.1 1,492,835 - -0.0 1,492,835 - +0.1 1,492,835 - -1.3 1,492,835 - +1.9 1,492,835 - -1.4 1,492,835 - +0.3 1,492,835 - -1.1 1,492,835 - -0.7 1,492,835 - +0.6 1,492,835 - +1.9 1,492,835 - +0.3 1,494,026 - AO353_06710 0.13 +1.7 1,494,026 - AO353_06710 0.13 +1.5 1,494,026 - AO353_06710 0.13 +1.4 1,494,026 - AO353_06710 0.13 +0.7 1,494,653 + -0.7 1,494,653 + -0.9 1,494,654 - -0.5 1,494,654 - -0.0 1,494,654 - -0.8 1,494,654 - +1.4 1,494,654 - -1.5 1,494,654 - -0.3 1,494,654 - -0.9 1,494,655 + -2.1 1,494,655 + -2.5 1,494,655 + -0.5 1,494,655 + -1.0 1,494,656 - -0.0 1,494,656 - -2.2 1,494,656 - -2.5 1,494,656 - -1.6 1,494,656 - -2.2 1,494,656 - -0.0 1,494,656 - -1.7 1,494,656 - -1.0
Or see this region's nucleotide sequence