Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03325 and AO353_03330 are separated by 67 nucleotides AO353_03330 and AO353_03335 are separated by 203 nucleotides AO353_03335 and AO353_03340 are separated by 21 nucleotides
AO353_03325: AO353_03325 - 3-hydroxybutyryl-CoA dehydratase, at 709,256 to 709,726
_03325
AO353_03330: AO353_03330 - aldehyde dehydrogenase, at 709,794 to 710,261
_03330
AO353_03335: AO353_03335 - hypothetical protein, at 710,465 to 710,701
_03335
AO353_03340: AO353_03340 - class V aminotransferase, at 710,723 to 711,904
_03340
Position (kb) 710
711 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 709.502 kb on + strand at 709.502 kb on + strand, within AO353_03325 at 709.502 kb on + strand at 709.503 kb on - strand at 709.560 kb on + strand, within AO353_03325 at 709.733 kb on + strand at 709.733 kb on + strand at 709.733 kb on + strand at 709.733 kb on + strand at 709.733 kb on + strand at 709.733 kb on + strand at 709.733 kb on + strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.734 kb on - strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.735 kb on + strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.736 kb on - strand at 709.787 kb on - strand at 709.787 kb on - strand at 709.795 kb on + strand at 709.795 kb on + strand at 709.796 kb on - strand at 709.796 kb on - strand at 709.796 kb on - strand at 709.861 kb on + strand, within AO353_03330 at 709.862 kb on - strand, within AO353_03330 at 709.930 kb on + strand, within AO353_03330 at 709.930 kb on + strand, within AO353_03330 at 709.930 kb on + strand, within AO353_03330 at 709.931 kb on - strand, within AO353_03330 at 709.931 kb on - strand, within AO353_03330 at 709.931 kb on - strand, within AO353_03330 at 710.033 kb on + strand, within AO353_03330 at 710.034 kb on - strand, within AO353_03330 at 710.034 kb on - strand, within AO353_03330 at 710.034 kb on - strand, within AO353_03330 at 710.034 kb on - strand, within AO353_03330 at 710.088 kb on - strand, within AO353_03330 at 710.246 kb on + strand at 710.246 kb on + strand at 710.246 kb on + strand at 710.247 kb on - strand at 710.247 kb on - strand at 710.247 kb on - strand at 710.290 kb on + strand at 710.290 kb on + strand at 710.290 kb on + strand at 710.291 kb on - strand at 710.291 kb on - strand at 710.291 kb on - strand at 710.295 kb on - strand at 710.295 kb on - strand at 710.352 kb on + strand at 710.352 kb on + strand at 710.353 kb on - strand at 710.353 kb on - strand at 710.357 kb on + strand at 710.364 kb on + strand at 710.365 kb on - strand at 710.387 kb on + strand at 710.387 kb on + strand at 710.387 kb on + strand at 710.388 kb on - strand at 710.388 kb on - strand at 710.388 kb on - strand at 710.430 kb on - strand at 710.519 kb on + strand, within AO353_03335 at 710.519 kb on + strand, within AO353_03335 at 710.519 kb on + strand, within AO353_03335 at 710.519 kb on + strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.520 kb on - strand, within AO353_03335 at 710.555 kb on + strand, within AO353_03335 at 710.555 kb on + strand, within AO353_03335 at 710.555 kb on + strand, within AO353_03335 at 710.556 kb on - strand, within AO353_03335 at 710.556 kb on - strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.628 kb on + strand, within AO353_03335 at 710.629 kb on - strand, within AO353_03335 at 710.629 kb on - strand, within AO353_03335 at 710.783 kb on + strand at 710.822 kb on + strand at 710.823 kb on - strand at 710.858 kb on + strand, within AO353_03340 at 710.859 kb on - strand, within AO353_03340 at 710.940 kb on + strand, within AO353_03340 at 710.940 kb on + strand, within AO353_03340 at 710.940 kb on + strand, within AO353_03340 at 710.941 kb on - strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.035 kb on + strand, within AO353_03340 at 711.036 kb on - strand, within AO353_03340 at 711.036 kb on - strand, within AO353_03340 at 711.036 kb on - strand, within AO353_03340 at 711.083 kb on + strand, within AO353_03340 at 711.084 kb on - strand, within AO353_03340 at 711.084 kb on - strand, within AO353_03340 at 711.191 kb on + strand, within AO353_03340 at 711.191 kb on + strand, within AO353_03340 at 711.192 kb on - strand, within AO353_03340 at 711.392 kb on + strand, within AO353_03340 at 711.392 kb on + strand, within AO353_03340 at 711.393 kb on - strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.440 kb on + strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.441 kb on - strand, within AO353_03340 at 711.466 kb on + strand, within AO353_03340 at 711.527 kb on + strand, within AO353_03340 at 711.528 kb on - strand, within AO353_03340 at 711.528 kb on - strand, within AO353_03340 at 711.552 kb on + strand, within AO353_03340 at 711.622 kb on + strand, within AO353_03340 at 711.622 kb on + strand, within AO353_03340 at 711.622 kb on + strand, within AO353_03340 at 711.623 kb on - strand, within AO353_03340 at 711.623 kb on - strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.626 kb on + strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340 at 711.627 kb on - strand, within AO353_03340
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 709,502 + +1.3 709,502 + AO353_03325 0.52 -0.3 709,502 + +0.1 709,503 - +1.3 709,560 + AO353_03325 0.65 -0.6 709,733 + +1.6 709,733 + +2.1 709,733 + -1.6 709,733 + -0.3 709,733 + +1.3 709,733 + -0.6 709,733 + -0.9 709,734 - -1.0 709,734 - -0.1 709,734 - +0.1 709,734 - +0.0 709,734 - +0.3 709,734 - -1.5 709,734 - -0.1 709,734 - -1.2 709,734 - +0.3 709,734 - -1.6 709,734 - -1.6 709,734 - -2.0 709,735 + +2.3 709,735 + -1.2 709,735 + -1.4 709,735 + -1.1 709,735 + -1.2 709,735 + -0.6 709,735 + +0.5 709,735 + +1.4 709,735 + +3.0 709,735 + +1.3 709,735 + -1.4 709,736 - -0.8 709,736 - -0.3 709,736 - -0.0 709,736 - +0.9 709,736 - -0.9 709,736 - -0.5 709,736 - -0.8 709,736 - -2.2 709,736 - +0.4 709,736 - -1.3 709,736 - -0.5 709,787 - -0.0 709,787 - +0.5 709,795 + +0.2 709,795 + -1.3 709,796 - -0.7 709,796 - +1.3 709,796 - -1.8 709,861 + AO353_03330 0.14 -2.8 709,862 - AO353_03330 0.15 -2.1 709,930 + AO353_03330 0.29 +0.9 709,930 + AO353_03330 0.29 +0.6 709,930 + AO353_03330 0.29 -0.6 709,931 - AO353_03330 0.29 -0.1 709,931 - AO353_03330 0.29 +0.3 709,931 - AO353_03330 0.29 +0.9 710,033 + AO353_03330 0.51 +0.9 710,034 - AO353_03330 0.51 +0.5 710,034 - AO353_03330 0.51 -1.2 710,034 - AO353_03330 0.51 +1.5 710,034 - AO353_03330 0.51 -1.3 710,088 - AO353_03330 0.63 -0.7 710,246 + +1.0 710,246 + +1.0 710,246 + -0.2 710,247 - -0.6 710,247 - +0.5 710,247 - -0.9 710,290 + -2.0 710,290 + -0.5 710,290 + +3.4 710,291 - +1.4 710,291 - +0.5 710,291 - -1.8 710,295 - -0.6 710,295 - -0.1 710,352 + -0.5 710,352 + -1.4 710,353 - -0.6 710,353 - -0.8 710,357 + -1.8 710,364 + +0.1 710,365 - +0.7 710,387 + -0.5 710,387 + -1.0 710,387 + +1.5 710,388 - +0.3 710,388 - -1.7 710,388 - -0.7 710,430 - +1.8 710,519 + AO353_03335 0.23 -2.2 710,519 + AO353_03335 0.23 +0.5 710,519 + AO353_03335 0.23 +1.5 710,519 + AO353_03335 0.23 -1.6 710,520 - AO353_03335 0.23 -0.2 710,520 - AO353_03335 0.23 -0.1 710,520 - AO353_03335 0.23 +0.2 710,520 - AO353_03335 0.23 -0.3 710,520 - AO353_03335 0.23 -2.1 710,520 - AO353_03335 0.23 -1.7 710,520 - AO353_03335 0.23 -1.5 710,555 + AO353_03335 0.38 -0.8 710,555 + AO353_03335 0.38 -1.4 710,555 + AO353_03335 0.38 -2.0 710,556 - AO353_03335 0.38 -2.2 710,556 - AO353_03335 0.38 -1.2 710,628 + AO353_03335 0.69 -1.1 710,628 + AO353_03335 0.69 -0.8 710,628 + AO353_03335 0.69 -0.6 710,628 + AO353_03335 0.69 -0.6 710,628 + AO353_03335 0.69 -1.9 710,628 + AO353_03335 0.69 -0.3 710,628 + AO353_03335 0.69 -2.8 710,629 - AO353_03335 0.69 -0.1 710,629 - AO353_03335 0.69 -3.0 710,783 + -0.3 710,822 + +1.2 710,823 - +2.4 710,858 + AO353_03340 0.11 +0.1 710,859 - AO353_03340 0.12 -0.5 710,940 + AO353_03340 0.18 -0.7 710,940 + AO353_03340 0.18 -1.8 710,940 + AO353_03340 0.18 -0.9 710,941 - AO353_03340 0.18 +0.2 711,035 + AO353_03340 0.26 -2.1 711,035 + AO353_03340 0.26 -0.3 711,035 + AO353_03340 0.26 -1.8 711,035 + AO353_03340 0.26 -1.8 711,035 + AO353_03340 0.26 +0.9 711,035 + AO353_03340 0.26 -0.5 711,036 - AO353_03340 0.26 +0.5 711,036 - AO353_03340 0.26 -0.5 711,036 - AO353_03340 0.26 -0.9 711,083 + AO353_03340 0.30 +1.0 711,084 - AO353_03340 0.31 -1.7 711,084 - AO353_03340 0.31 -1.7 711,191 + AO353_03340 0.40 -0.2 711,191 + AO353_03340 0.40 +4.1 711,192 - AO353_03340 0.40 +0.5 711,392 + AO353_03340 0.57 -1.5 711,392 + AO353_03340 0.57 +1.5 711,393 - AO353_03340 0.57 -1.8 711,440 + AO353_03340 0.61 -1.4 711,440 + AO353_03340 0.61 -0.9 711,440 + AO353_03340 0.61 +2.0 711,440 + AO353_03340 0.61 -2.0 711,440 + AO353_03340 0.61 -0.8 711,440 + AO353_03340 0.61 +0.8 711,441 - AO353_03340 0.61 -1.1 711,441 - AO353_03340 0.61 -1.9 711,441 - AO353_03340 0.61 -1.2 711,441 - AO353_03340 0.61 -2.9 711,441 - AO353_03340 0.61 +1.8 711,441 - AO353_03340 0.61 +1.1 711,466 + AO353_03340 0.63 +2.9 711,527 + AO353_03340 0.68 -1.1 711,528 - AO353_03340 0.68 +0.4 711,528 - AO353_03340 0.68 +0.9 711,552 + AO353_03340 0.70 +1.1 711,622 + AO353_03340 0.76 +1.5 711,622 + AO353_03340 0.76 -1.3 711,622 + AO353_03340 0.76 -0.9 711,623 - AO353_03340 0.76 -1.9 711,623 - AO353_03340 0.76 -0.8 711,626 + AO353_03340 0.76 -1.2 711,626 + AO353_03340 0.76 +0.9 711,626 + AO353_03340 0.76 -1.0 711,626 + AO353_03340 0.76 -0.1 711,626 + AO353_03340 0.76 -1.1 711,626 + AO353_03340 0.76 +1.1 711,627 - AO353_03340 0.76 -0.8 711,627 - AO353_03340 0.76 +1.1 711,627 - AO353_03340 0.76 +0.1 711,627 - AO353_03340 0.76 -0.4 711,627 - AO353_03340 0.76 -1.4 711,627 - AO353_03340 0.76 -2.6
Or see this region's nucleotide sequence