Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01660 and AO353_01665 are separated by 232 nucleotides AO353_01665 and AO353_01670 are separated by 120 nucleotides
AO353_01660: AO353_01660 - short-chain dehydrogenase, at 349,683 to 350,444
_01660
AO353_01665: AO353_01665 - NADP-dependent oxidoreductase, at 350,677 to 351,681
_01665
AO353_01670: AO353_01670 - orotidine 5'-phosphate decarboxylase, at 351,802 to 352,536
_01670
Position (kb) 350
351
352 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 349.756 kb on + strand at 349.756 kb on + strand at 349.756 kb on + strand at 349.756 kb on + strand at 349.756 kb on + strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.757 kb on - strand at 349.849 kb on + strand, within AO353_01660 at 349.975 kb on + strand, within AO353_01660 at 349.975 kb on + strand, within AO353_01660 at 349.975 kb on + strand, within AO353_01660 at 349.975 kb on + strand, within AO353_01660 at 349.975 kb on + strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.976 kb on - strand, within AO353_01660 at 349.979 kb on + strand, within AO353_01660 at 349.979 kb on + strand, within AO353_01660 at 349.979 kb on + strand, within AO353_01660 at 349.979 kb on + strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 349.980 kb on - strand, within AO353_01660 at 350.068 kb on + strand, within AO353_01660 at 350.069 kb on - strand, within AO353_01660 at 350.069 kb on - strand, within AO353_01660 at 350.069 kb on - strand, within AO353_01660 at 350.069 kb on - strand, within AO353_01660 at 350.069 kb on - strand, within AO353_01660 at 350.186 kb on - strand, within AO353_01660 at 350.186 kb on - strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.200 kb on + strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.201 kb on - strand, within AO353_01660 at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.448 kb on + strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.449 kb on - strand at 350.488 kb on + strand at 350.488 kb on + strand at 350.488 kb on + strand at 350.488 kb on + strand at 350.488 kb on + strand at 350.488 kb on + strand at 350.489 kb on - strand at 350.489 kb on - strand at 350.489 kb on - strand at 350.489 kb on - strand at 350.518 kb on + strand at 350.518 kb on + strand at 350.518 kb on + strand at 350.518 kb on + strand at 350.519 kb on - strand at 350.519 kb on - strand at 350.541 kb on + strand at 350.541 kb on + strand at 350.541 kb on + strand at 350.542 kb on - strand at 350.542 kb on - strand at 350.575 kb on - strand at 350.598 kb on - strand at 350.641 kb on + strand at 350.752 kb on + strand at 350.752 kb on + strand at 350.752 kb on + strand at 350.752 kb on + strand at 350.753 kb on - strand at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.809 kb on + strand, within AO353_01665 at 350.810 kb on - strand, within AO353_01665 at 350.810 kb on - strand, within AO353_01665 at 350.810 kb on - strand, within AO353_01665 at 350.860 kb on + strand, within AO353_01665 at 350.860 kb on + strand, within AO353_01665 at 350.860 kb on + strand, within AO353_01665 at 350.860 kb on + strand, within AO353_01665 at 350.860 kb on + strand, within AO353_01665 at 350.861 kb on - strand, within AO353_01665 at 350.861 kb on - strand, within AO353_01665 at 350.861 kb on - strand, within AO353_01665 at 350.861 kb on - strand, within AO353_01665 at 350.953 kb on + strand, within AO353_01665 at 350.983 kb on + strand, within AO353_01665 at 350.983 kb on + strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 350.984 kb on - strand, within AO353_01665 at 351.010 kb on + strand, within AO353_01665 at 351.010 kb on + strand, within AO353_01665 at 351.010 kb on + strand, within AO353_01665 at 351.011 kb on - strand, within AO353_01665 at 351.011 kb on - strand, within AO353_01665 at 351.011 kb on - strand, within AO353_01665 at 351.011 kb on - strand, within AO353_01665 at 351.011 kb on - strand, within AO353_01665 at 351.013 kb on - strand, within AO353_01665 at 351.013 kb on - strand, within AO353_01665 at 351.046 kb on + strand, within AO353_01665 at 351.046 kb on + strand, within AO353_01665 at 351.046 kb on + strand, within AO353_01665 at 351.083 kb on - strand, within AO353_01665 at 351.083 kb on - strand, within AO353_01665 at 351.083 kb on - strand, within AO353_01665 at 351.083 kb on - strand, within AO353_01665 at 351.274 kb on + strand, within AO353_01665 at 351.274 kb on + strand, within AO353_01665 at 351.275 kb on - strand, within AO353_01665 at 351.275 kb on - strand, within AO353_01665 at 351.275 kb on - strand, within AO353_01665 at 351.326 kb on + strand, within AO353_01665 at 351.326 kb on + strand, within AO353_01665 at 351.326 kb on + strand, within AO353_01665 at 351.327 kb on - strand, within AO353_01665 at 351.327 kb on - strand, within AO353_01665 at 351.334 kb on + strand, within AO353_01665 at 351.334 kb on + strand, within AO353_01665 at 351.335 kb on - strand, within AO353_01665 at 351.335 kb on - strand, within AO353_01665 at 351.335 kb on - strand, within AO353_01665 at 351.357 kb on + strand, within AO353_01665 at 351.427 kb on + strand, within AO353_01665 at 351.427 kb on + strand, within AO353_01665 at 351.427 kb on + strand, within AO353_01665 at 351.427 kb on + strand, within AO353_01665 at 351.428 kb on - strand, within AO353_01665 at 351.428 kb on - strand, within AO353_01665 at 351.428 kb on - strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.463 kb on + strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.464 kb on - strand, within AO353_01665 at 351.467 kb on + strand, within AO353_01665 at 351.467 kb on + strand, within AO353_01665 at 351.468 kb on - strand, within AO353_01665 at 351.712 kb on + strand at 351.712 kb on + strand at 351.712 kb on + strand at 351.712 kb on + strand at 351.713 kb on - strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.754 kb on + strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.755 kb on - strand at 351.760 kb on + strand at 351.760 kb on + strand at 351.761 kb on - strand at 351.761 kb on - strand at 351.789 kb on + strand at 352.540 kb on - strand at 352.576 kb on + strand at 352.577 kb on - strand at 352.577 kb on - strand at 352.610 kb on + strand at 352.610 kb on + strand at 352.611 kb on - strand at 352.611 kb on - strand at 352.611 kb on - strand at 352.611 kb on - strand at 352.646 kb on + strand at 352.646 kb on + strand at 352.646 kb on + strand at 352.646 kb on + strand at 352.646 kb on + strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand at 352.647 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 349,756 + -1.2 349,756 + +1.5 349,756 + -0.5 349,756 + -0.1 349,756 + -0.5 349,757 - +0.3 349,757 - +0.3 349,757 - -2.2 349,757 - -0.8 349,757 - +0.9 349,757 - +2.7 349,757 - -2.0 349,757 - -1.8 349,757 - -0.7 349,849 + AO353_01660 0.22 +1.7 349,975 + AO353_01660 0.38 +1.6 349,975 + AO353_01660 0.38 -0.0 349,975 + AO353_01660 0.38 -0.0 349,975 + AO353_01660 0.38 +0.5 349,975 + AO353_01660 0.38 -1.6 349,976 - AO353_01660 0.38 +0.4 349,976 - AO353_01660 0.38 -2.3 349,976 - AO353_01660 0.38 -0.7 349,976 - AO353_01660 0.38 +0.7 349,976 - AO353_01660 0.38 -0.0 349,976 - AO353_01660 0.38 -1.2 349,976 - AO353_01660 0.38 +1.4 349,976 - AO353_01660 0.38 +0.6 349,976 - AO353_01660 0.38 -1.6 349,979 + AO353_01660 0.39 -1.4 349,979 + AO353_01660 0.39 +0.3 349,979 + AO353_01660 0.39 -1.1 349,979 + AO353_01660 0.39 -1.8 349,980 - AO353_01660 0.39 -1.5 349,980 - AO353_01660 0.39 +0.3 349,980 - AO353_01660 0.39 +0.9 349,980 - AO353_01660 0.39 -1.1 349,980 - AO353_01660 0.39 -1.0 349,980 - AO353_01660 0.39 -1.2 350,068 + AO353_01660 0.51 +1.2 350,069 - AO353_01660 0.51 -1.1 350,069 - AO353_01660 0.51 -1.3 350,069 - AO353_01660 0.51 -2.0 350,069 - AO353_01660 0.51 +1.1 350,069 - AO353_01660 0.51 +3.2 350,186 - AO353_01660 0.66 -0.3 350,186 - AO353_01660 0.66 +0.2 350,200 + AO353_01660 0.68 -0.5 350,200 + AO353_01660 0.68 -0.3 350,200 + AO353_01660 0.68 -2.0 350,200 + AO353_01660 0.68 -0.6 350,200 + AO353_01660 0.68 -2.5 350,200 + AO353_01660 0.68 +1.4 350,200 + AO353_01660 0.68 -1.5 350,200 + AO353_01660 0.68 -2.4 350,200 + AO353_01660 0.68 -3.4 350,200 + AO353_01660 0.68 -0.5 350,200 + AO353_01660 0.68 +0.4 350,201 - AO353_01660 0.68 -0.3 350,201 - AO353_01660 0.68 -2.0 350,201 - AO353_01660 0.68 +2.2 350,201 - AO353_01660 0.68 +1.9 350,201 - AO353_01660 0.68 +2.3 350,201 - AO353_01660 0.68 +2.9 350,201 - AO353_01660 0.68 +2.9 350,201 - AO353_01660 0.68 -0.0 350,201 - AO353_01660 0.68 -0.6 350,448 + -0.1 350,448 + -2.3 350,448 + -1.1 350,448 + -0.7 350,448 + +1.1 350,448 + -1.6 350,448 + -0.5 350,448 + +0.4 350,449 - -0.4 350,449 - -1.5 350,449 - +1.1 350,449 - -1.1 350,449 - +1.3 350,449 - -1.7 350,449 - -1.1 350,449 - +1.9 350,488 + -2.2 350,488 + -1.0 350,488 + -1.5 350,488 + -1.1 350,488 + -1.9 350,488 + -1.0 350,489 - +0.1 350,489 - -1.5 350,489 - +1.9 350,489 - -0.8 350,518 + -0.2 350,518 + -2.4 350,518 + +0.2 350,518 + +0.4 350,519 - -0.6 350,519 - -0.2 350,541 + -0.9 350,541 + -0.7 350,541 + -1.1 350,542 - -2.5 350,542 - -0.2 350,575 - -2.0 350,598 - -1.7 350,641 + +0.7 350,752 + -1.7 350,752 + -0.3 350,752 + +0.1 350,752 + -0.3 350,753 - +0.0 350,809 + AO353_01665 0.13 +1.4 350,809 + AO353_01665 0.13 -2.6 350,809 + AO353_01665 0.13 -0.4 350,809 + AO353_01665 0.13 +0.2 350,809 + AO353_01665 0.13 +1.0 350,809 + AO353_01665 0.13 -1.8 350,809 + AO353_01665 0.13 -1.2 350,810 - AO353_01665 0.13 -0.5 350,810 - AO353_01665 0.13 -0.5 350,810 - AO353_01665 0.13 +0.0 350,860 + AO353_01665 0.18 +0.3 350,860 + AO353_01665 0.18 -0.1 350,860 + AO353_01665 0.18 +0.4 350,860 + AO353_01665 0.18 -1.7 350,860 + AO353_01665 0.18 -2.5 350,861 - AO353_01665 0.18 -0.3 350,861 - AO353_01665 0.18 +1.8 350,861 - AO353_01665 0.18 +0.3 350,861 - AO353_01665 0.18 -0.1 350,953 + AO353_01665 0.27 +1.2 350,983 + AO353_01665 0.30 +0.1 350,983 + AO353_01665 0.30 -1.5 350,984 - AO353_01665 0.31 -0.8 350,984 - AO353_01665 0.31 -2.0 350,984 - AO353_01665 0.31 -1.0 350,984 - AO353_01665 0.31 -1.0 350,984 - AO353_01665 0.31 -0.2 350,984 - AO353_01665 0.31 +1.8 350,984 - AO353_01665 0.31 +0.2 351,010 + AO353_01665 0.33 +2.7 351,010 + AO353_01665 0.33 -2.3 351,010 + AO353_01665 0.33 -1.0 351,011 - AO353_01665 0.33 -0.7 351,011 - AO353_01665 0.33 -0.1 351,011 - AO353_01665 0.33 -0.7 351,011 - AO353_01665 0.33 +1.6 351,011 - AO353_01665 0.33 -1.2 351,013 - AO353_01665 0.33 -0.9 351,013 - AO353_01665 0.33 +1.5 351,046 + AO353_01665 0.37 -0.5 351,046 + AO353_01665 0.37 +1.3 351,046 + AO353_01665 0.37 -1.5 351,083 - AO353_01665 0.40 -1.0 351,083 - AO353_01665 0.40 -1.8 351,083 - AO353_01665 0.40 +0.1 351,083 - AO353_01665 0.40 -1.1 351,274 + AO353_01665 0.59 -2.3 351,274 + AO353_01665 0.59 -1.4 351,275 - AO353_01665 0.60 +0.0 351,275 - AO353_01665 0.60 +2.4 351,275 - AO353_01665 0.60 +0.7 351,326 + AO353_01665 0.65 +0.1 351,326 + AO353_01665 0.65 -2.9 351,326 + AO353_01665 0.65 -0.9 351,327 - AO353_01665 0.65 +1.0 351,327 - AO353_01665 0.65 -1.1 351,334 + AO353_01665 0.65 +0.2 351,334 + AO353_01665 0.65 -1.6 351,335 - AO353_01665 0.65 +0.1 351,335 - AO353_01665 0.65 -1.4 351,335 - AO353_01665 0.65 -2.6 351,357 + AO353_01665 0.68 -0.9 351,427 + AO353_01665 0.75 +0.3 351,427 + AO353_01665 0.75 -2.1 351,427 + AO353_01665 0.75 +0.1 351,427 + AO353_01665 0.75 -3.1 351,428 - AO353_01665 0.75 -1.0 351,428 - AO353_01665 0.75 +1.3 351,428 - AO353_01665 0.75 +1.5 351,463 + AO353_01665 0.78 -0.7 351,463 + AO353_01665 0.78 -0.7 351,463 + AO353_01665 0.78 -0.8 351,463 + AO353_01665 0.78 -0.4 351,463 + AO353_01665 0.78 -0.1 351,463 + AO353_01665 0.78 -1.3 351,463 + AO353_01665 0.78 -0.9 351,463 + AO353_01665 0.78 +0.3 351,463 + AO353_01665 0.78 -1.5 351,464 - AO353_01665 0.78 +0.2 351,464 - AO353_01665 0.78 -0.7 351,464 - AO353_01665 0.78 -1.9 351,464 - AO353_01665 0.78 +1.3 351,464 - AO353_01665 0.78 +0.9 351,464 - AO353_01665 0.78 +0.6 351,464 - AO353_01665 0.78 +0.9 351,467 + AO353_01665 0.79 -0.3 351,467 + AO353_01665 0.79 -0.7 351,468 - AO353_01665 0.79 -0.8 351,712 + +2.5 351,712 + +0.4 351,712 + +0.7 351,712 + -1.5 351,713 - +0.5 351,754 + +1.3 351,754 + +1.2 351,754 + -1.6 351,754 + -2.0 351,754 + -0.5 351,754 + +1.4 351,754 + -0.7 351,754 + -0.2 351,754 + -0.9 351,754 + -0.7 351,754 + -1.0 351,754 + +0.9 351,755 - -0.0 351,755 - +2.2 351,755 - -1.4 351,755 - -2.1 351,755 - -1.7 351,755 - -1.1 351,755 - +0.1 351,755 - -0.7 351,755 - +1.2 351,755 - +3.3 351,755 - +0.9 351,755 - +3.1 351,755 - +1.8 351,755 - +0.2 351,755 - +0.2 351,755 - -0.7 351,755 - +1.0 351,755 - +0.8 351,755 - +1.7 351,755 - -1.8 351,760 + +0.9 351,760 + -0.1 351,761 - +1.1 351,761 - +4.0 351,789 + +0.7 352,540 - -0.8 352,576 + +0.1 352,577 - -0.6 352,577 - -2.3 352,610 + +1.4 352,610 + +2.0 352,611 - +0.5 352,611 - -1.1 352,611 - -2.4 352,611 - -1.5 352,646 + -0.1 352,646 + -0.1 352,646 + +3.8 352,646 + +2.1 352,646 + +1.5 352,647 - +2.8 352,647 - -1.1 352,647 - +0.9 352,647 - +1.1 352,647 - -2.2 352,647 - -0.2 352,647 - -0.5 352,647 - -0.8
Or see this region's nucleotide sequence