Experiment: Cadmium chloride hemipentahydrate 0.00005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_655 and Pf6N2E2_656 are separated by 150 nucleotides Pf6N2E2_656 and Pf6N2E2_657 are separated by 113 nucleotides Pf6N2E2_657 and Pf6N2E2_658 are separated by 199 nucleotides
Pf6N2E2_655 - Filamentous haemagglutinin family outer membrane protein associated with VreARI signalling system, at 746,494 to 759,111
_655
Pf6N2E2_656 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 759,262 to 761,835
_656
Pf6N2E2_657 - hypothetical protein, at 761,949 to 762,065
_657
Pf6N2E2_658 - sensor histidine kinase, at 762,265 to 763,575
_658
Position (kb) 759
760
761
762 Strain fitness (log2 ratio) -2
-1
0
1
2 at 758.422 kb on + strand at 758.422 kb on + strand at 758.422 kb on + strand at 758.423 kb on - strand at 758.423 kb on - strand at 758.430 kb on + strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.487 kb on - strand at 758.546 kb on - strand at 758.567 kb on - strand at 759.010 kb on + strand at 759.011 kb on - strand at 759.109 kb on + strand at 759.110 kb on - strand at 759.169 kb on - strand at 759.169 kb on - strand at 759.219 kb on + strand at 759.220 kb on - strand at 759.326 kb on + strand at 759.327 kb on - strand at 759.416 kb on - strand at 759.704 kb on - strand, within Pf6N2E2_656 at 759.704 kb on - strand, within Pf6N2E2_656 at 759.817 kb on - strand, within Pf6N2E2_656 at 759.817 kb on - strand, within Pf6N2E2_656 at 759.959 kb on + strand, within Pf6N2E2_656 at 760.011 kb on - strand, within Pf6N2E2_656 at 760.052 kb on + strand, within Pf6N2E2_656 at 760.053 kb on - strand, within Pf6N2E2_656 at 760.053 kb on - strand, within Pf6N2E2_656 at 760.091 kb on + strand, within Pf6N2E2_656 at 760.092 kb on - strand, within Pf6N2E2_656 at 760.092 kb on - strand, within Pf6N2E2_656 at 760.178 kb on + strand, within Pf6N2E2_656 at 760.179 kb on - strand, within Pf6N2E2_656 at 760.179 kb on - strand, within Pf6N2E2_656 at 760.345 kb on - strand, within Pf6N2E2_656 at 760.638 kb on + strand, within Pf6N2E2_656 at 760.638 kb on + strand, within Pf6N2E2_656 at 760.664 kb on + strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.827 kb on - strand, within Pf6N2E2_656 at 760.944 kb on - strand, within Pf6N2E2_656 at 761.300 kb on + strand, within Pf6N2E2_656 at 761.301 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.490 kb on - strand, within Pf6N2E2_656 at 761.495 kb on + strand, within Pf6N2E2_656 at 761.496 kb on - strand, within Pf6N2E2_656 at 761.504 kb on + strand, within Pf6N2E2_656 at 761.534 kb on + strand, within Pf6N2E2_656 at 761.835 kb on + strand at 761.841 kb on + strand at 761.841 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.882 kb on - strand at 761.903 kb on + strand at 761.903 kb on + strand at 761.904 kb on - strand at 761.907 kb on + strand at 761.908 kb on - strand at 762.151 kb on + strand at 762.151 kb on + strand at 762.152 kb on - strand at 762.737 kb on + strand, within Pf6N2E2_658
Per-strain Table
Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.00005 M remove 758,422 + +0.0 758,422 + -1.0 758,422 + +0.0 758,423 - -0.0 758,423 - -1.5 758,430 + -0.6 758,487 - +0.4 758,487 - +0.1 758,487 - -0.2 758,487 - -0.7 758,546 - -0.4 758,567 - -0.4 759,010 + -0.2 759,011 - +0.1 759,109 + -0.1 759,110 - +0.5 759,169 - -0.8 759,169 - +0.9 759,219 + -1.4 759,220 - +0.1 759,326 + +0.0 759,327 - -1.3 759,416 - +0.2 759,704 - Pf6N2E2_656 0.17 +0.3 759,704 - Pf6N2E2_656 0.17 -0.0 759,817 - Pf6N2E2_656 0.22 -0.7 759,817 - Pf6N2E2_656 0.22 +0.2 759,959 + Pf6N2E2_656 0.27 -2.2 760,011 - Pf6N2E2_656 0.29 +0.5 760,052 + Pf6N2E2_656 0.31 +0.5 760,053 - Pf6N2E2_656 0.31 +0.8 760,053 - Pf6N2E2_656 0.31 -2.2 760,091 + Pf6N2E2_656 0.32 -0.7 760,092 - Pf6N2E2_656 0.32 -0.1 760,092 - Pf6N2E2_656 0.32 +0.4 760,178 + Pf6N2E2_656 0.36 +0.8 760,179 - Pf6N2E2_656 0.36 -0.5 760,179 - Pf6N2E2_656 0.36 +0.4 760,345 - Pf6N2E2_656 0.42 -1.6 760,638 + Pf6N2E2_656 0.53 +1.0 760,638 + Pf6N2E2_656 0.53 -1.3 760,664 + Pf6N2E2_656 0.54 +0.7 760,827 - Pf6N2E2_656 0.61 +2.0 760,827 - Pf6N2E2_656 0.61 +1.4 760,827 - Pf6N2E2_656 0.61 +0.0 760,827 - Pf6N2E2_656 0.61 +1.5 760,944 - Pf6N2E2_656 0.65 +0.8 761,300 + Pf6N2E2_656 0.79 +1.2 761,301 - Pf6N2E2_656 0.79 -0.2 761,448 - Pf6N2E2_656 0.85 +1.1 761,448 - Pf6N2E2_656 0.85 +1.9 761,490 - Pf6N2E2_656 0.87 +0.2 761,495 + Pf6N2E2_656 0.87 -0.1 761,496 - Pf6N2E2_656 0.87 +2.0 761,504 + Pf6N2E2_656 0.87 +0.7 761,534 + Pf6N2E2_656 0.88 +0.5 761,835 + -0.9 761,841 + -1.0 761,841 + -0.3 761,881 + +0.2 761,881 + +0.9 761,881 + -1.3 761,881 + +1.4 761,881 + -0.0 761,882 - +0.2 761,903 + -0.9 761,903 + -0.6 761,904 - -1.3 761,907 + -0.6 761,908 - -1.2 762,151 + +0.2 762,151 + -0.4 762,152 - +0.4 762,737 + Pf6N2E2_658 0.36 +0.3
Or see this region's nucleotide sequence