Experiment: Cadmium chloride hemipentahydrate 0.00005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2033 and Pf6N2E2_2034 are separated by 5 nucleotides Pf6N2E2_2034 and Pf6N2E2_2035 are separated by 67 nucleotides Pf6N2E2_2035 and Pf6N2E2_2036 are separated by 10 nucleotides Pf6N2E2_2036 and Pf6N2E2_2037 are separated by 56 nucleotides
Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425
_2033
Pf6N2E2_2034 - Methyltransferase, at 2,423,431 to 2,424,294
_2034
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Pf6N2E2_2036 - Antibiotic biosynthesis monooxygenase, at 2,425,377 to 2,425,754
_2036
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Position (kb)
2424
2425
2426 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.388 kb on - strand at 2423.388 kb on - strand at 2423.389 kb on + strand at 2423.390 kb on - strand at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.889 kb on + strand, within Pf6N2E2_2034 at 2424.013 kb on - strand, within Pf6N2E2_2034 at 2424.275 kb on + strand at 2424.364 kb on - strand at 2424.364 kb on - strand at 2424.600 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.604 kb on - strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.771 kb on + strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2425.345 kb on - strand at 2425.345 kb on - strand at 2425.367 kb on - strand at 2425.367 kb on - strand at 2425.447 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.522 kb on + strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.523 kb on - strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.659 kb on + strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.660 kb on - strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.667 kb on + strand, within Pf6N2E2_2036 at 2425.668 kb on - strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.671 kb on + strand, within Pf6N2E2_2036 at 2425.672 kb on - strand, within Pf6N2E2_2036 at 2425.718 kb on + strand at 2425.726 kb on - strand at 2425.726 kb on - strand at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2426.229 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.348 kb on - strand, within Pf6N2E2_2037
Per-strain Table
Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.00005 M remove 2,423,387 + +0.6 2,423,387 + +1.1 2,423,387 + -0.2 2,423,388 - +0.0 2,423,388 - -2.2 2,423,389 + -1.1 2,423,390 - +0.6 2,423,567 - Pf6N2E2_2034 0.16 -0.1 2,423,567 - Pf6N2E2_2034 0.16 -0.7 2,423,889 + Pf6N2E2_2034 0.53 +0.1 2,424,013 - Pf6N2E2_2034 0.67 -0.4 2,424,275 + +0.3 2,424,364 - +0.0 2,424,364 - -1.7 2,424,600 + Pf6N2E2_2035 0.24 -0.4 2,424,603 + Pf6N2E2_2035 0.24 +0.2 2,424,603 + Pf6N2E2_2035 0.24 -0.2 2,424,603 + Pf6N2E2_2035 0.24 +0.1 2,424,604 - Pf6N2E2_2035 0.24 -0.2 2,424,644 + Pf6N2E2_2035 0.28 +0.6 2,424,644 + Pf6N2E2_2035 0.28 -0.5 2,424,644 + Pf6N2E2_2035 0.28 +0.1 2,424,645 - Pf6N2E2_2035 0.28 -1.7 2,424,645 - Pf6N2E2_2035 0.28 -0.4 2,424,645 - Pf6N2E2_2035 0.28 -0.2 2,424,645 - Pf6N2E2_2035 0.28 -1.0 2,424,771 + Pf6N2E2_2035 0.41 -1.2 2,424,772 - Pf6N2E2_2035 0.41 -0.8 2,424,772 - Pf6N2E2_2035 0.41 +0.9 2,424,772 - Pf6N2E2_2035 0.41 -2.1 2,424,772 - Pf6N2E2_2035 0.41 -0.6 2,424,772 - Pf6N2E2_2035 0.41 -0.2 2,424,772 - Pf6N2E2_2035 0.41 -1.5 2,424,888 + Pf6N2E2_2035 0.52 -0.7 2,424,888 + Pf6N2E2_2035 0.52 -1.7 2,424,888 + Pf6N2E2_2035 0.52 -1.3 2,424,888 + Pf6N2E2_2035 0.52 +0.1 2,424,889 - Pf6N2E2_2035 0.52 -0.1 2,424,889 - Pf6N2E2_2035 0.52 +0.2 2,425,345 - +0.3 2,425,345 - +0.5 2,425,367 - +0.1 2,425,367 - +1.3 2,425,447 + Pf6N2E2_2036 0.19 -0.1 2,425,522 + Pf6N2E2_2036 0.38 +0.2 2,425,522 + +0.6 2,425,522 + Pf6N2E2_2036 0.38 +2.2 2,425,522 + Pf6N2E2_2036 0.38 +0.1 2,425,522 + Pf6N2E2_2036 0.38 -0.6 2,425,522 + Pf6N2E2_2036 0.38 -0.6 2,425,522 + Pf6N2E2_2036 0.38 -0.1 2,425,522 + Pf6N2E2_2036 0.38 +1.2 2,425,523 - Pf6N2E2_2036 0.39 -1.0 2,425,523 - Pf6N2E2_2036 0.39 -0.9 2,425,523 - Pf6N2E2_2036 0.39 -0.5 2,425,523 - Pf6N2E2_2036 0.39 +0.6 2,425,523 - Pf6N2E2_2036 0.39 +2.1 2,425,659 + Pf6N2E2_2036 0.75 +0.5 2,425,659 + Pf6N2E2_2036 0.75 -0.5 2,425,659 + Pf6N2E2_2036 0.75 -0.5 2,425,659 + Pf6N2E2_2036 0.75 +1.2 2,425,659 + Pf6N2E2_2036 0.75 +0.6 2,425,659 + Pf6N2E2_2036 0.75 -0.4 2,425,660 - Pf6N2E2_2036 0.75 +0.0 2,425,660 - Pf6N2E2_2036 0.75 -0.4 2,425,660 - Pf6N2E2_2036 0.75 +0.9 2,425,660 - Pf6N2E2_2036 0.75 +1.0 2,425,660 - Pf6N2E2_2036 0.75 +0.4 2,425,667 + Pf6N2E2_2036 0.77 -0.5 2,425,667 + Pf6N2E2_2036 0.77 +1.0 2,425,667 + Pf6N2E2_2036 0.77 -0.1 2,425,667 + Pf6N2E2_2036 0.77 -0.3 2,425,667 + Pf6N2E2_2036 0.77 -0.6 2,425,667 + Pf6N2E2_2036 0.77 -2.3 2,425,668 - Pf6N2E2_2036 0.77 +1.6 2,425,671 + Pf6N2E2_2036 0.78 +0.9 2,425,671 + Pf6N2E2_2036 0.78 -1.0 2,425,671 + Pf6N2E2_2036 0.78 -0.3 2,425,672 - Pf6N2E2_2036 0.78 +0.0 2,425,718 + +0.4 2,425,726 - +3.2 2,425,726 - +1.2 2,425,915 - Pf6N2E2_2037 0.12 -1.5 2,425,915 - Pf6N2E2_2037 0.12 +2.0 2,426,229 + Pf6N2E2_2037 0.47 +0.5 2,426,241 + Pf6N2E2_2037 0.48 -0.4 2,426,241 + Pf6N2E2_2037 0.48 +0.6 2,426,241 + Pf6N2E2_2037 0.48 +0.8 2,426,241 + Pf6N2E2_2037 0.48 -0.5 2,426,241 + Pf6N2E2_2037 0.48 +0.1 2,426,242 - Pf6N2E2_2037 0.48 -0.2 2,426,242 - Pf6N2E2_2037 0.48 -0.2 2,426,242 - Pf6N2E2_2037 0.48 -0.7 2,426,348 - Pf6N2E2_2037 0.60 -0.3
Or see this region's nucleotide sequence