Experiment: Cadmium chloride hemipentahydrate 0.00005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1742 and Pf6N2E2_1743 overlap by 1 nucleotides Pf6N2E2_1743 and Pf6N2E2_1744 are separated by 151 nucleotides
Pf6N2E2_1742 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,054,767 to 2,056,158
_1742
Pf6N2E2_1743 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,056,158 to 2,057,264
_1743
Pf6N2E2_1744 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,057,416 to 2,058,468
_1744
Position (kb)
2056
2057
2058 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2055.341 kb on - strand, within Pf6N2E2_1742 at 2055.678 kb on - strand, within Pf6N2E2_1742 at 2055.728 kb on + strand, within Pf6N2E2_1742 at 2055.729 kb on - strand, within Pf6N2E2_1742 at 2055.750 kb on + strand, within Pf6N2E2_1742 at 2055.805 kb on + strand, within Pf6N2E2_1742 at 2055.942 kb on - strand, within Pf6N2E2_1742 at 2056.227 kb on + strand at 2056.228 kb on - strand at 2056.444 kb on - strand, within Pf6N2E2_1743 at 2056.714 kb on - strand, within Pf6N2E2_1743 at 2056.758 kb on + strand, within Pf6N2E2_1743 at 2057.248 kb on + strand at 2057.248 kb on + strand at 2057.249 kb on - strand at 2057.249 kb on - strand at 2057.288 kb on + strand at 2057.289 kb on - strand at 2057.289 kb on - strand at 2057.348 kb on + strand at 2057.348 kb on + strand at 2057.387 kb on + strand at 2057.388 kb on - strand at 2057.388 kb on - strand at 2057.388 kb on - strand at 2057.471 kb on + strand at 2057.471 kb on + strand at 2057.472 kb on - strand at 2057.605 kb on + strand, within Pf6N2E2_1744 at 2057.605 kb on + strand, within Pf6N2E2_1744 at 2057.606 kb on - strand, within Pf6N2E2_1744 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2058.116 kb on - strand, within Pf6N2E2_1744 at 2058.185 kb on - strand, within Pf6N2E2_1744 at 2058.238 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744
Per-strain Table
Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.00005 M remove 2,055,341 - Pf6N2E2_1742 0.41 -0.3 2,055,678 - Pf6N2E2_1742 0.65 -0.7 2,055,728 + Pf6N2E2_1742 0.69 -1.3 2,055,729 - Pf6N2E2_1742 0.69 +0.1 2,055,750 + Pf6N2E2_1742 0.71 +0.6 2,055,805 + Pf6N2E2_1742 0.75 +0.5 2,055,942 - Pf6N2E2_1742 0.84 -3.0 2,056,227 + -0.3 2,056,228 - +1.0 2,056,444 - Pf6N2E2_1743 0.26 -1.1 2,056,714 - Pf6N2E2_1743 0.50 -0.8 2,056,758 + Pf6N2E2_1743 0.54 -0.6 2,057,248 + -1.1 2,057,248 + -0.7 2,057,249 - -0.6 2,057,249 - +1.0 2,057,288 + +2.0 2,057,289 - -1.1 2,057,289 - +0.5 2,057,348 + -0.5 2,057,348 + +0.9 2,057,387 + +0.4 2,057,388 - +0.2 2,057,388 - +0.7 2,057,388 - -0.9 2,057,471 + -0.8 2,057,471 + +0.5 2,057,472 - +0.3 2,057,605 + Pf6N2E2_1744 0.18 -0.2 2,057,605 + Pf6N2E2_1744 0.18 -0.1 2,057,606 - Pf6N2E2_1744 0.18 -0.5 2,057,821 + Pf6N2E2_1744 0.38 +0.3 2,057,821 + Pf6N2E2_1744 0.38 -1.6 2,058,116 - Pf6N2E2_1744 0.66 -1.2 2,058,185 - Pf6N2E2_1744 0.73 +0.2 2,058,238 + Pf6N2E2_1744 0.78 +0.9 2,058,253 + Pf6N2E2_1744 0.79 +0.8 2,058,253 + Pf6N2E2_1744 0.79 +1.5 2,058,253 + Pf6N2E2_1744 0.79 -0.6
Or see this region's nucleotide sequence