Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25315

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25305 and BWI76_RS25310 are separated by 60 nucleotidesBWI76_RS25310 and BWI76_RS25315 are separated by 415 nucleotidesBWI76_RS25315 and BWI76_RS25320 are separated by 9 nucleotides BWI76_RS25305: BWI76_RS25305 - TIGR01212 family radical SAM protein, at 5,259,459 to 5,260,394 _RS25305 BWI76_RS25310: BWI76_RS25310 - hypothetical protein, at 5,260,455 to 5,260,652 _RS25310 BWI76_RS25315: BWI76_RS25315 - glutamate synthase large subunit, at 5,261,068 to 5,265,528 _RS25315 BWI76_RS25320: BWI76_RS25320 - glutamate synthase, at 5,265,538 to 5,266,956 _RS25320 Position (kb) 5261 5262 5263 5264 5265 5266Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 5260.709 kb on - strandat 5260.709 kb on - strandat 5260.716 kb on + strandat 5260.718 kb on - strandat 5260.824 kb on - strandat 5260.855 kb on + strandat 5260.866 kb on + strandat 5260.981 kb on + strandat 5260.995 kb on + strandat 5261.038 kb on + strandat 5261.042 kb on + strandat 5261.047 kb on + strandat 5261.085 kb on - strandat 5261.161 kb on + strandat 5261.183 kb on - strandat 5261.469 kb on + strandat 5261.742 kb on - strand, within BWI76_RS25315at 5261.763 kb on + strand, within BWI76_RS25315at 5262.061 kb on - strand, within BWI76_RS25315at 5262.079 kb on - strand, within BWI76_RS25315at 5262.109 kb on + strand, within BWI76_RS25315at 5262.283 kb on + strand, within BWI76_RS25315at 5262.702 kb on - strand, within BWI76_RS25315at 5262.809 kb on + strand, within BWI76_RS25315at 5262.905 kb on + strand, within BWI76_RS25315at 5262.967 kb on + strand, within BWI76_RS25315at 5262.970 kb on - strand, within BWI76_RS25315at 5263.082 kb on + strand, within BWI76_RS25315at 5263.416 kb on + strand, within BWI76_RS25315at 5263.416 kb on + strand, within BWI76_RS25315at 5263.940 kb on + strand, within BWI76_RS25315at 5263.940 kb on + strand, within BWI76_RS25315at 5264.107 kb on + strand, within BWI76_RS25315at 5264.222 kb on - strand, within BWI76_RS25315at 5264.327 kb on - strand, within BWI76_RS25315at 5264.380 kb on - strand, within BWI76_RS25315at 5264.659 kb on + strand, within BWI76_RS25315at 5264.672 kb on - strand, within BWI76_RS25315at 5264.763 kb on - strand, within BWI76_RS25315at 5264.768 kb on + strand, within BWI76_RS25315at 5264.919 kb on - strand, within BWI76_RS25315at 5265.056 kb on - strand, within BWI76_RS25315at 5265.502 kb on + strandat 5265.748 kb on - strand, within BWI76_RS25320at 5265.967 kb on - strand, within BWI76_RS25320at 5266.009 kb on + strand, within BWI76_RS25320at 5266.228 kb on + strand, within BWI76_RS25320at 5266.268 kb on - strand, within BWI76_RS25320at 5266.308 kb on + strand, within BWI76_RS25320at 5266.432 kb on + strand, within BWI76_RS25320at 5266.432 kb on + strand, within BWI76_RS25320

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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5,260,709 - +0.1
5,260,709 - +0.1
5,260,716 + -0.7
5,260,718 - -1.0
5,260,824 - +0.0
5,260,855 + -1.3
5,260,866 + -0.2
5,260,981 + +0.0
5,260,995 + -0.4
5,261,038 + -0.5
5,261,042 + -0.6
5,261,047 + -0.7
5,261,085 - -3.7
5,261,161 + -1.0
5,261,183 - -3.0
5,261,469 + -2.4
5,261,742 - BWI76_RS25315 0.15 -2.0
5,261,763 + BWI76_RS25315 0.16 -5.0
5,262,061 - BWI76_RS25315 0.22 -1.3
5,262,079 - BWI76_RS25315 0.23 -1.2
5,262,109 + BWI76_RS25315 0.23 -1.9
5,262,283 + BWI76_RS25315 0.27 -1.9
5,262,702 - BWI76_RS25315 0.37 -1.2
5,262,809 + BWI76_RS25315 0.39 -2.4
5,262,905 + BWI76_RS25315 0.41 -2.5
5,262,967 + BWI76_RS25315 0.43 +1.2
5,262,970 - BWI76_RS25315 0.43 -1.9
5,263,082 + BWI76_RS25315 0.45 -1.3
5,263,416 + BWI76_RS25315 0.53 -2.6
5,263,416 + BWI76_RS25315 0.53 -1.1
5,263,940 + BWI76_RS25315 0.64 -1.1
5,263,940 + BWI76_RS25315 0.64 -1.3
5,264,107 + BWI76_RS25315 0.68 -1.5
5,264,222 - BWI76_RS25315 0.71 -1.6
5,264,327 - BWI76_RS25315 0.73 -0.4
5,264,380 - BWI76_RS25315 0.74 -1.4
5,264,659 + BWI76_RS25315 0.80 -2.0
5,264,672 - BWI76_RS25315 0.81 -2.2
5,264,763 - BWI76_RS25315 0.83 +0.1
5,264,768 + BWI76_RS25315 0.83 -2.3
5,264,919 - BWI76_RS25315 0.86 -1.7
5,265,056 - BWI76_RS25315 0.89 +0.4
5,265,502 + +3.7
5,265,748 - BWI76_RS25320 0.15 -3.1
5,265,967 - BWI76_RS25320 0.30 -1.2
5,266,009 + BWI76_RS25320 0.33 -1.8
5,266,228 + BWI76_RS25320 0.49 -1.9
5,266,268 - BWI76_RS25320 0.51 -0.8
5,266,308 + BWI76_RS25320 0.54 -1.9
5,266,432 + BWI76_RS25320 0.63 -2.3
5,266,432 + BWI76_RS25320 0.63 -2.1

Or see this region's nucleotide sequence