Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25280

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25265 and BWI76_RS25270 are separated by 22 nucleotidesBWI76_RS25270 and BWI76_RS25275 are separated by 65 nucleotidesBWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotidesBWI76_RS25280 and BWI76_RS25285 overlap by 4 nucleotidesBWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotides BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280 _RS25265 BWI76_RS25270: BWI76_RS25270 - ribosome hibernation promoting factor, at 5,253,303 to 5,253,590 _RS25270 BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144 _RS25275 BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044 _RS25280 BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313 _RS25285 BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092 _RS25290 Position (kb) 5254 5255 5256Strain fitness (log2 ratio) -2 -1 0 1 2at 5253.303 kb on + strandat 5253.477 kb on + strand, within BWI76_RS25270at 5253.483 kb on + strand, within BWI76_RS25270at 5253.502 kb on + strand, within BWI76_RS25270at 5254.184 kb on + strandat 5254.201 kb on - strandat 5254.555 kb on + strand, within BWI76_RS25280at 5255.053 kb on - strandat 5255.069 kb on + strand, within BWI76_RS25285at 5255.356 kb on - strandat 5255.396 kb on - strandat 5255.680 kb on - strand, within BWI76_RS25290at 5255.899 kb on - strand, within BWI76_RS25290at 5255.989 kb on + strand, within BWI76_RS25290at 5255.989 kb on + strand, within BWI76_RS25290at 5255.994 kb on + strand, within BWI76_RS25290at 5255.997 kb on - strand, within BWI76_RS25290at 5256.013 kb on + strand, within BWI76_RS25290at 5256.021 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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5,253,303 + +1.7
5,253,477 + BWI76_RS25270 0.60 +0.4
5,253,483 + BWI76_RS25270 0.62 +2.0
5,253,502 + BWI76_RS25270 0.69 -0.2
5,254,184 + +0.3
5,254,201 - -1.1
5,254,555 + BWI76_RS25280 0.43 -2.1
5,255,053 - +2.2
5,255,069 + BWI76_RS25285 0.10 -0.2
5,255,356 - -1.3
5,255,396 - +0.6
5,255,680 - BWI76_RS25290 0.43 +0.9
5,255,899 - BWI76_RS25290 0.73 -0.2
5,255,989 + BWI76_RS25290 0.86 -0.7
5,255,989 + BWI76_RS25290 0.86 -1.2
5,255,994 + BWI76_RS25290 0.86 +0.9
5,255,997 - BWI76_RS25290 0.87 +0.5
5,256,013 + BWI76_RS25290 0.89 -0.3
5,256,021 - -0.5

Or see this region's nucleotide sequence