Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25265 and BWI76_RS25270 are separated by 22 nucleotides BWI76_RS25270 and BWI76_RS25275 are separated by 65 nucleotides BWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotides BWI76_RS25280 and BWI76_RS25285 overlap by 4 nucleotides BWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotides
BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280
_RS25265
BWI76_RS25270: BWI76_RS25270 - ribosome hibernation promoting factor, at 5,253,303 to 5,253,590
_RS25270
BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144
_RS25275
BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044
_RS25280
BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313
_RS25285
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
Position (kb) 5254
5255
5256 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5253.303 kb on + strand at 5253.477 kb on + strand, within BWI76_RS25270 at 5253.483 kb on + strand, within BWI76_RS25270 at 5253.502 kb on + strand, within BWI76_RS25270 at 5254.184 kb on + strand at 5254.201 kb on - strand at 5254.555 kb on + strand, within BWI76_RS25280 at 5255.053 kb on - strand at 5255.069 kb on + strand, within BWI76_RS25285 at 5255.356 kb on - strand at 5255.396 kb on - strand at 5255.680 kb on - strand, within BWI76_RS25290 at 5255.899 kb on - strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 5,253,303 + +1.7 5,253,477 + BWI76_RS25270 0.60 +0.4 5,253,483 + BWI76_RS25270 0.62 +2.0 5,253,502 + BWI76_RS25270 0.69 -0.2 5,254,184 + +0.3 5,254,201 - -1.1 5,254,555 + BWI76_RS25280 0.43 -2.1 5,255,053 - +2.2 5,255,069 + BWI76_RS25285 0.10 -0.2 5,255,356 - -1.3 5,255,396 - +0.6 5,255,680 - BWI76_RS25290 0.43 +0.9 5,255,899 - BWI76_RS25290 0.73 -0.2 5,255,989 + BWI76_RS25290 0.86 -0.7 5,255,989 + BWI76_RS25290 0.86 -1.2 5,255,994 + BWI76_RS25290 0.86 +0.9 5,255,997 - BWI76_RS25290 0.87 +0.5 5,256,013 + BWI76_RS25290 0.89 -0.3 5,256,021 - -0.5
Or see this region's nucleotide sequence