Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25130

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25115 and BWI76_RS25120 are separated by 153 nucleotidesBWI76_RS25120 and BWI76_RS25125 are separated by 14 nucleotidesBWI76_RS25125 and BWI76_RS25130 are separated by 229 nucleotidesBWI76_RS25130 and BWI76_RS25135 overlap by 8 nucleotidesBWI76_RS25135 and BWI76_RS25140 are separated by 97 nucleotides BWI76_RS25115: BWI76_RS25115 - argininosuccinate synthase, at 5,227,953 to 5,229,296 _RS25115 BWI76_RS25120: BWI76_RS25120 - tRNA-Leu, at 5,229,450 to 5,229,536 _RS25120 BWI76_RS25125: BWI76_RS25125 - preprotein translocase subunit SecG, at 5,229,551 to 5,229,880 _RS25125 BWI76_RS25130: BWI76_RS25130 - phosphoglucosamine mutase, at 5,230,110 to 5,231,447 _RS25130 BWI76_RS25135: BWI76_RS25135 - dihydropteroate synthase, at 5,231,440 to 5,232,288 _RS25135 BWI76_RS25140: BWI76_RS25140 - ATP-dependent metalloprotease, at 5,232,386 to 5,234,320 _RS25140 Position (kb) 5230 5231 5232Strain fitness (log2 ratio) -1 0 1at 5230.108 kb on + strandat 5232.337 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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5,230,108 + +0.7
5,232,337 - -0.4

Or see this region's nucleotide sequence