Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20965

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20960 and BWI76_RS20965 are separated by 265 nucleotidesBWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotidesBWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotides BWI76_RS20960: BWI76_RS20960 - ROK family protein, at 4,381,211 to 4,382,104 _RS20960 BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407 _RS20965 BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045 _RS20970 BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447 _RS20975 Position (kb) 4382 4383 4384Strain fitness (log2 ratio) -3 -2 -1 0 1at 4381.419 kb on + strand, within BWI76_RS20960at 4381.427 kb on - strand, within BWI76_RS20960at 4381.475 kb on - strand, within BWI76_RS20960at 4381.839 kb on - strand, within BWI76_RS20960at 4381.839 kb on - strand, within BWI76_RS20960at 4382.355 kb on + strandat 4382.628 kb on - strand, within BWI76_RS20965at 4382.639 kb on - strand, within BWI76_RS20965at 4383.163 kb on - strand, within BWI76_RS20965at 4383.337 kb on - strandat 4383.346 kb on - strandat 4383.379 kb on + strandat 4383.431 kb on + strandat 4383.859 kb on + strand, within BWI76_RS20970at 4384.255 kb on + strand, within BWI76_RS20975

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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4,381,419 + BWI76_RS20960 0.23 -0.1
4,381,427 - BWI76_RS20960 0.24 -0.0
4,381,475 - BWI76_RS20960 0.30 -0.1
4,381,839 - BWI76_RS20960 0.70 +0.6
4,381,839 - BWI76_RS20960 0.70 -0.9
4,382,355 + -1.3
4,382,628 - BWI76_RS20965 0.25 -2.5
4,382,639 - BWI76_RS20965 0.26 -3.4
4,383,163 - BWI76_RS20965 0.76 -2.9
4,383,337 - -1.4
4,383,346 - -3.3
4,383,379 + -0.3
4,383,431 + -0.3
4,383,859 + BWI76_RS20970 0.71 -0.9
4,384,255 + BWI76_RS20975 0.12 +0.2

Or see this region's nucleotide sequence