Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08385

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotidesBWI76_RS08380 and BWI76_RS08385 are separated by 45 nucleotidesBWI76_RS08385 and BWI76_RS08390 are separated by 437 nucleotidesBWI76_RS08390 and BWI76_RS08395 overlap by 17 nucleotides BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825 _RS08375 BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632 _RS08380 BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961 _RS08385 BWI76_RS08390: BWI76_RS08390 - hypothetical protein, at 1,775,399 to 1,775,617 _RS08390 BWI76_RS08395: BWI76_RS08395 - succinate dehydrogenase cytochrome b556 large subunit, at 1,775,601 to 1,776,005 _RS08395 Position (kb) 1773 1774 1775Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1772.904 kb on + strandat 1772.912 kb on - strandat 1773.069 kb on + strand, within BWI76_RS08380at 1773.220 kb on + strand, within BWI76_RS08380at 1773.794 kb on + strandat 1773.862 kb on - strand, within BWI76_RS08385at 1774.346 kb on + strand, within BWI76_RS08385at 1774.604 kb on - strand, within BWI76_RS08385at 1775.091 kb on + strandat 1775.099 kb on - strandat 1775.114 kb on + strandat 1775.183 kb on + strandat 1775.196 kb on - strandat 1775.207 kb on + strandat 1775.215 kb on + strandat 1775.450 kb on + strand, within BWI76_RS08390at 1775.461 kb on - strand, within BWI76_RS08390at 1775.658 kb on - strandat 1775.929 kb on - strand, within BWI76_RS08395

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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1,772,904 + +0.0
1,772,912 - -0.0
1,773,069 + BWI76_RS08380 0.29 -0.2
1,773,220 + BWI76_RS08380 0.48 +0.5
1,773,794 + +0.8
1,773,862 - BWI76_RS08385 0.14 -2.2
1,774,346 + BWI76_RS08385 0.52 -3.3
1,774,604 - BWI76_RS08385 0.72 -0.2
1,775,091 + -2.6
1,775,099 - -5.5
1,775,114 + -3.9
1,775,183 + -3.6
1,775,196 - -1.4
1,775,207 + -1.7
1,775,215 + -3.7
1,775,450 + BWI76_RS08390 0.23 -3.1
1,775,461 - BWI76_RS08390 0.28 -2.1
1,775,658 - +0.2
1,775,929 - BWI76_RS08395 0.81 -4.2

Or see this region's nucleotide sequence