Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05465

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotidesBWI76_RS05460 and BWI76_RS05465 are separated by 245 nucleotidesBWI76_RS05465 and BWI76_RS05470 are separated by 182 nucleotides BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226 _RS05455 BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435 _RS05460 BWI76_RS05465: BWI76_RS05465 - amidohydrolase, at 1,145,681 to 1,146,451 _RS05465 BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078 _RS05470 Position (kb) 1145 1146 1147Strain fitness (log2 ratio) -1 0 1at 1145.308 kb on + strandat 1145.520 kb on + strandat 1145.628 kb on - strandat 1145.997 kb on - strand, within BWI76_RS05465at 1146.396 kb on - strandat 1146.490 kb on + strandat 1146.982 kb on - strand, within BWI76_RS05470at 1147.030 kb on + strand, within BWI76_RS05470at 1147.132 kb on - strand, within BWI76_RS05470at 1147.231 kb on + strand, within BWI76_RS05470at 1147.317 kb on + strand, within BWI76_RS05470at 1147.347 kb on - strand, within BWI76_RS05470at 1147.395 kb on - strand, within BWI76_RS05470at 1147.421 kb on - strand, within BWI76_RS05470at 1147.428 kb on + strand, within BWI76_RS05470

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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1,145,308 + -0.2
1,145,520 + +0.7
1,145,628 - -0.3
1,145,997 - BWI76_RS05465 0.41 -1.3
1,146,396 - -1.2
1,146,490 + +0.5
1,146,982 - BWI76_RS05470 0.14 -0.1
1,147,030 + BWI76_RS05470 0.16 +0.4
1,147,132 - BWI76_RS05470 0.20 +1.0
1,147,231 + BWI76_RS05470 0.24 +0.3
1,147,317 + BWI76_RS05470 0.28 +0.7
1,147,347 - BWI76_RS05470 0.29 +1.0
1,147,395 - BWI76_RS05470 0.31 +0.7
1,147,421 - BWI76_RS05470 0.32 +0.2
1,147,428 + BWI76_RS05470 0.32 +0.4

Or see this region's nucleotide sequence