Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_28055

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_28050 and AO353_28055 are separated by 2 nucleotidesAO353_28055 and AO353_28060 overlap by 4 nucleotides AO353_28050: AO353_28050 - exonuclease SbcC, at 6,113,945 to 6,115,921 _28050 AO353_28055: AO353_28055 - hypothetical protein, at 6,115,924 to 6,116,496 _28055 AO353_28060: AO353_28060 - hypothetical protein, at 6,116,493 to 6,117,707 _28060 Position (kb) 6115 6116 6117Strain fitness (log2 ratio) -2 -1 0 1 2 3at 6115.172 kb on + strand, within AO353_28050at 6115.323 kb on - strand, within AO353_28050at 6115.654 kb on - strand, within AO353_28050at 6115.669 kb on - strand, within AO353_28050at 6115.670 kb on + strand, within AO353_28050at 6115.831 kb on - strandat 6115.953 kb on - strandat 6116.136 kb on - strand, within AO353_28055at 6116.142 kb on + strand, within AO353_28055at 6116.143 kb on - strand, within AO353_28055at 6116.143 kb on - strand, within AO353_28055at 6116.197 kb on - strand, within AO353_28055at 6116.276 kb on + strand, within AO353_28055at 6116.473 kb on + strandat 6116.586 kb on + strandat 6116.735 kb on - strand, within AO353_28060at 6116.735 kb on - strand, within AO353_28060at 6116.767 kb on - strand, within AO353_28060at 6116.970 kb on - strand, within AO353_28060at 6116.989 kb on + strand, within AO353_28060at 6117.063 kb on + strand, within AO353_28060at 6117.125 kb on - strand, within AO353_28060at 6117.178 kb on + strand, within AO353_28060at 6117.178 kb on + strand, within AO353_28060at 6117.179 kb on - strand, within AO353_28060at 6117.181 kb on - strand, within AO353_28060at 6117.181 kb on - strand, within AO353_28060at 6117.181 kb on - strand, within AO353_28060at 6117.222 kb on - strand, within AO353_28060at 6117.222 kb on - strand, within AO353_28060at 6117.294 kb on + strand, within AO353_28060at 6117.362 kb on + strand, within AO353_28060at 6117.393 kb on - strand, within AO353_28060at 6117.403 kb on - strand, within AO353_28060

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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6,115,172 + AO353_28050 0.62 +0.6
6,115,323 - AO353_28050 0.70 -0.1
6,115,654 - AO353_28050 0.86 -1.4
6,115,669 - AO353_28050 0.87 +0.6
6,115,670 + AO353_28050 0.87 -1.3
6,115,831 - +1.8
6,115,953 - -0.4
6,116,136 - AO353_28055 0.37 -1.2
6,116,142 + AO353_28055 0.38 -0.6
6,116,143 - AO353_28055 0.38 -2.0
6,116,143 - AO353_28055 0.38 -2.6
6,116,197 - AO353_28055 0.48 -0.7
6,116,276 + AO353_28055 0.61 -1.5
6,116,473 + +0.2
6,116,586 + +2.3
6,116,735 - AO353_28060 0.20 -0.9
6,116,735 - AO353_28060 0.20 -0.7
6,116,767 - AO353_28060 0.23 -0.1
6,116,970 - AO353_28060 0.39 -0.3
6,116,989 + AO353_28060 0.41 +1.2
6,117,063 + AO353_28060 0.47 -1.4
6,117,125 - AO353_28060 0.52 +3.1
6,117,178 + AO353_28060 0.56 -0.2
6,117,178 + AO353_28060 0.56 -1.7
6,117,179 - AO353_28060 0.56 -0.3
6,117,181 - AO353_28060 0.57 +0.6
6,117,181 - AO353_28060 0.57 -0.1
6,117,181 - AO353_28060 0.57 +1.3
6,117,222 - AO353_28060 0.60 +0.3
6,117,222 - AO353_28060 0.60 -2.7
6,117,294 + AO353_28060 0.66 -2.0
6,117,362 + AO353_28060 0.72 +0.3
6,117,393 - AO353_28060 0.74 +0.7
6,117,403 - AO353_28060 0.75 -0.7

Or see this region's nucleotide sequence