Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_24935 and AO353_24940 overlap by 4 nucleotides AO353_24940 and AO353_24945 are separated by 303 nucleotides AO353_24945 and AO353_24950 are separated by 24 nucleotides
AO353_24935: AO353_24935 - cysteine sulfinate desulfinase, at 5,499,788 to 5,501,062
_24935
AO353_24940: AO353_24940 - nitrogen fixation protein NifU, at 5,501,059 to 5,501,517
_24940
AO353_24945: AO353_24945 - hypothetical protein, at 5,501,821 to 5,502,357
_24945
AO353_24950: AO353_24950 - thioredoxin, at 5,502,382 to 5,504,370
_24950
Position (kb) 5501
5502
5503 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5500.888 kb on + strand, within AO353_24935 at 5500.995 kb on - strand at 5501.200 kb on + strand, within AO353_24940 at 5501.201 kb on - strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.346 kb on + strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.347 kb on - strand, within AO353_24940 at 5501.515 kb on + strand at 5501.515 kb on + strand at 5501.515 kb on + strand at 5501.515 kb on + strand at 5501.516 kb on - strand at 5501.538 kb on - strand at 5501.550 kb on + strand at 5501.767 kb on + strand at 5501.819 kb on - strand at 5502.086 kb on - strand, within AO353_24945 at 5502.086 kb on - strand, within AO353_24945 at 5502.130 kb on + strand, within AO353_24945 at 5502.130 kb on + strand, within AO353_24945 at 5502.131 kb on - strand, within AO353_24945 at 5502.131 kb on - strand, within AO353_24945 at 5502.131 kb on - strand, within AO353_24945 at 5502.238 kb on + strand, within AO353_24945 at 5502.238 kb on + strand, within AO353_24945 at 5502.239 kb on - strand, within AO353_24945 at 5502.239 kb on - strand, within AO353_24945 at 5502.239 kb on - strand, within AO353_24945 at 5502.239 kb on - strand, within AO353_24945 at 5502.239 kb on - strand, within AO353_24945 at 5502.610 kb on - strand, within AO353_24950 at 5502.652 kb on + strand, within AO353_24950 at 5502.652 kb on + strand, within AO353_24950 at 5502.652 kb on + strand, within AO353_24950 at 5502.652 kb on + strand, within AO353_24950 at 5502.652 kb on + strand, within AO353_24950 at 5502.653 kb on - strand, within AO353_24950 at 5502.653 kb on - strand, within AO353_24950 at 5502.653 kb on - strand, within AO353_24950 at 5502.745 kb on + strand, within AO353_24950 at 5502.746 kb on - strand, within AO353_24950 at 5502.746 kb on - strand, within AO353_24950 at 5502.746 kb on - strand, within AO353_24950 at 5502.904 kb on - strand, within AO353_24950 at 5503.036 kb on + strand, within AO353_24950 at 5503.111 kb on + strand, within AO353_24950 at 5503.111 kb on + strand, within AO353_24950 at 5503.112 kb on - strand, within AO353_24950 at 5503.112 kb on - strand, within AO353_24950 at 5503.142 kb on - strand, within AO353_24950 at 5503.216 kb on + strand, within AO353_24950 at 5503.217 kb on - strand, within AO353_24950 at 5503.229 kb on - strand, within AO353_24950 at 5503.282 kb on + strand, within AO353_24950 at 5503.282 kb on + strand, within AO353_24950 at 5503.282 kb on + strand, within AO353_24950 at 5503.282 kb on + strand, within AO353_24950 at 5503.282 kb on + strand, within AO353_24950 at 5503.283 kb on - strand, within AO353_24950 at 5503.283 kb on - strand, within AO353_24950
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,500,888 + AO353_24935 0.86 -0.5 5,500,995 - +1.4 5,501,200 + AO353_24940 0.31 +0.3 5,501,201 - AO353_24940 0.31 +2.6 5,501,346 + AO353_24940 0.63 -0.7 5,501,346 + AO353_24940 0.63 +0.5 5,501,346 + AO353_24940 0.63 -1.0 5,501,346 + AO353_24940 0.63 +0.2 5,501,346 + AO353_24940 0.63 +2.1 5,501,346 + AO353_24940 0.63 +2.4 5,501,346 + AO353_24940 0.63 +2.5 5,501,346 + AO353_24940 0.63 -2.9 5,501,346 + AO353_24940 0.63 -2.5 5,501,346 + AO353_24940 0.63 -0.4 5,501,347 - AO353_24940 0.63 -2.3 5,501,347 - AO353_24940 0.63 +2.4 5,501,347 - AO353_24940 0.63 -1.8 5,501,347 - +0.5 5,501,347 - AO353_24940 0.63 -2.6 5,501,347 - AO353_24940 0.63 -1.5 5,501,347 - AO353_24940 0.63 +0.8 5,501,347 - AO353_24940 0.63 +0.6 5,501,515 + -0.8 5,501,515 + -1.8 5,501,515 + -1.1 5,501,515 + +0.9 5,501,516 - +1.9 5,501,538 - +0.4 5,501,550 + +0.6 5,501,767 + +0.9 5,501,819 - -1.6 5,502,086 - AO353_24945 0.49 +0.2 5,502,086 - AO353_24945 0.49 -1.1 5,502,130 + AO353_24945 0.58 +0.6 5,502,130 + AO353_24945 0.58 -1.9 5,502,131 - AO353_24945 0.58 +2.3 5,502,131 - AO353_24945 0.58 -2.4 5,502,131 - AO353_24945 0.58 -0.1 5,502,238 + AO353_24945 0.78 +2.3 5,502,238 + AO353_24945 0.78 -2.0 5,502,239 - AO353_24945 0.78 +1.9 5,502,239 - AO353_24945 0.78 -0.1 5,502,239 - AO353_24945 0.78 +0.3 5,502,239 - AO353_24945 0.78 +1.7 5,502,239 - AO353_24945 0.78 +0.5 5,502,610 - AO353_24950 0.11 -1.5 5,502,652 + AO353_24950 0.14 +0.4 5,502,652 + AO353_24950 0.14 -0.1 5,502,652 + AO353_24950 0.14 -1.9 5,502,652 + AO353_24950 0.14 -1.5 5,502,652 + AO353_24950 0.14 +0.6 5,502,653 - AO353_24950 0.14 +1.1 5,502,653 - AO353_24950 0.14 -2.1 5,502,653 - AO353_24950 0.14 -0.1 5,502,745 + AO353_24950 0.18 -0.9 5,502,746 - AO353_24950 0.18 -0.9 5,502,746 - AO353_24950 0.18 -1.3 5,502,746 - AO353_24950 0.18 +0.5 5,502,904 - AO353_24950 0.26 -1.3 5,503,036 + AO353_24950 0.33 +0.5 5,503,111 + AO353_24950 0.37 +0.9 5,503,111 + AO353_24950 0.37 -0.5 5,503,112 - AO353_24950 0.37 +0.1 5,503,112 - AO353_24950 0.37 +0.6 5,503,142 - AO353_24950 0.38 +1.4 5,503,216 + AO353_24950 0.42 -1.5 5,503,217 - AO353_24950 0.42 -1.8 5,503,229 - AO353_24950 0.43 +1.1 5,503,282 + AO353_24950 0.45 +1.0 5,503,282 + AO353_24950 0.45 -1.9 5,503,282 + AO353_24950 0.45 -1.8 5,503,282 + AO353_24950 0.45 -2.0 5,503,282 + AO353_24950 0.45 -1.4 5,503,283 - AO353_24950 0.45 -0.9 5,503,283 - AO353_24950 0.45 +3.6
Or see this region's nucleotide sequence