Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_21555 and AO353_21560 are separated by 226 nucleotides AO353_21560 and AO353_21565 are separated by 62 nucleotides AO353_21565 and AO353_21570 are separated by 18 nucleotides AO353_21570 and AO353_21575 overlap by 4 nucleotides
AO353_21555: AO353_21555 - type VI secretion protein, at 4,718,531 to 4,719,103
_21555
AO353_21560: AO353_21560 - type VI secretion protein, at 4,719,330 to 4,719,779
_21560
AO353_21565: AO353_21565 - type VI secretion protein, at 4,719,842 to 4,721,173
_21565
AO353_21570: AO353_21570 - type VI secretion system protein ImpK, at 4,721,192 to 4,721,959
_21570
AO353_21575: AO353_21575 - type VI secretion system protein ImpL, at 4,721,956 to 4,725,774
_21575
Position (kb)
4719
4720
4721
4722 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4719.026 kb on - strand, within AO353_21555 at 4719.026 kb on - strand, within AO353_21555 at 4719.029 kb on + strand, within AO353_21555 at 4719.029 kb on + strand, within AO353_21555 at 4719.029 kb on + strand, within AO353_21555 at 4719.030 kb on - strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.042 kb on + strand, within AO353_21555 at 4719.043 kb on - strand, within AO353_21555 at 4719.043 kb on - strand, within AO353_21555 at 4719.043 kb on - strand, within AO353_21555 at 4719.043 kb on - strand, within AO353_21555 at 4719.092 kb on - strand at 4719.092 kb on - strand at 4719.177 kb on + strand at 4719.178 kb on - strand at 4719.178 kb on - strand at 4719.182 kb on + strand at 4719.251 kb on + strand at 4719.251 kb on + strand at 4719.251 kb on + strand at 4719.263 kb on + strand at 4719.274 kb on + strand at 4719.274 kb on + strand at 4719.275 kb on - strand at 4719.277 kb on - strand at 4719.277 kb on - strand at 4719.277 kb on - strand at 4719.348 kb on - strand at 4719.368 kb on - strand at 4719.368 kb on - strand at 4719.675 kb on + strand, within AO353_21560 at 4719.675 kb on + strand, within AO353_21560 at 4719.675 kb on + strand, within AO353_21560 at 4719.675 kb on + strand, within AO353_21560 at 4719.675 kb on + strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.676 kb on - strand, within AO353_21560 at 4719.677 kb on + strand, within AO353_21560 at 4719.678 kb on - strand, within AO353_21560 at 4719.842 kb on - strand at 4719.853 kb on - strand at 4720.154 kb on + strand, within AO353_21565 at 4720.191 kb on + strand, within AO353_21565 at 4720.191 kb on + strand, within AO353_21565 at 4720.191 kb on + strand, within AO353_21565 at 4720.192 kb on - strand, within AO353_21565 at 4720.192 kb on - strand, within AO353_21565 at 4720.192 kb on - strand, within AO353_21565 at 4720.194 kb on + strand, within AO353_21565 at 4720.195 kb on - strand, within AO353_21565 at 4720.273 kb on + strand, within AO353_21565 at 4720.374 kb on + strand, within AO353_21565 at 4720.385 kb on + strand at 4720.385 kb on + strand, within AO353_21565 at 4720.386 kb on - strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.622 kb on + strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.623 kb on - strand, within AO353_21565 at 4720.725 kb on - strand, within AO353_21565 at 4720.734 kb on + strand, within AO353_21565 at 4720.735 kb on - strand, within AO353_21565 at 4721.015 kb on + strand, within AO353_21565 at 4721.015 kb on + strand, within AO353_21565 at 4721.033 kb on - strand, within AO353_21565 at 4721.432 kb on + strand, within AO353_21570 at 4721.432 kb on + strand, within AO353_21570 at 4721.433 kb on - strand, within AO353_21570 at 4721.433 kb on - strand, within AO353_21570 at 4721.511 kb on + strand, within AO353_21570 at 4721.525 kb on + strand, within AO353_21570 at 4721.525 kb on + strand, within AO353_21570 at 4721.525 kb on + strand at 4721.525 kb on + strand, within AO353_21570 at 4721.526 kb on - strand, within AO353_21570 at 4721.526 kb on - strand, within AO353_21570 at 4721.526 kb on - strand, within AO353_21570 at 4721.526 kb on - strand, within AO353_21570 at 4721.618 kb on + strand, within AO353_21570 at 4721.618 kb on + strand, within AO353_21570 at 4721.618 kb on + strand, within AO353_21570 at 4721.618 kb on + strand, within AO353_21570 at 4721.634 kb on + strand, within AO353_21570 at 4722.070 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,719,026 - AO353_21555 0.86 -2.0 4,719,026 - AO353_21555 0.86 +1.6 4,719,029 + AO353_21555 0.87 +1.3 4,719,029 + AO353_21555 0.87 +1.8 4,719,029 + AO353_21555 0.87 +2.3 4,719,030 - AO353_21555 0.87 -1.1 4,719,042 + AO353_21555 0.89 -1.9 4,719,042 + AO353_21555 0.89 -1.8 4,719,042 + AO353_21555 0.89 -1.4 4,719,042 + AO353_21555 0.89 +0.7 4,719,042 + AO353_21555 0.89 -0.3 4,719,042 + AO353_21555 0.89 +2.1 4,719,042 + AO353_21555 0.89 -0.3 4,719,043 - AO353_21555 0.89 -2.5 4,719,043 - AO353_21555 0.89 +0.9 4,719,043 - AO353_21555 0.89 +2.5 4,719,043 - AO353_21555 0.89 +1.8 4,719,092 - +0.9 4,719,092 - -0.5 4,719,177 + -0.1 4,719,178 - -1.3 4,719,178 - +1.6 4,719,182 + +2.9 4,719,251 + -0.8 4,719,251 + +1.1 4,719,251 + -0.5 4,719,263 + -0.1 4,719,274 + +0.3 4,719,274 + -0.8 4,719,275 - +1.1 4,719,277 - -1.0 4,719,277 - -1.1 4,719,277 - -1.7 4,719,348 - -1.8 4,719,368 - +1.9 4,719,368 - -0.2 4,719,675 + AO353_21560 0.77 -0.8 4,719,675 + AO353_21560 0.77 -1.9 4,719,675 + AO353_21560 0.77 -1.1 4,719,675 + AO353_21560 0.77 +1.5 4,719,675 + AO353_21560 0.77 -0.7 4,719,676 - AO353_21560 0.77 -1.6 4,719,676 - AO353_21560 0.77 -0.7 4,719,676 - AO353_21560 0.77 -0.8 4,719,676 - AO353_21560 0.77 +1.3 4,719,676 - AO353_21560 0.77 -1.7 4,719,676 - AO353_21560 0.77 +1.3 4,719,676 - AO353_21560 0.77 -1.3 4,719,676 - AO353_21560 0.77 -0.1 4,719,677 + AO353_21560 0.77 -1.5 4,719,678 - AO353_21560 0.77 -2.3 4,719,842 - -1.5 4,719,853 - +0.2 4,720,154 + AO353_21565 0.23 +0.7 4,720,191 + AO353_21565 0.26 +1.6 4,720,191 + AO353_21565 0.26 -0.5 4,720,191 + AO353_21565 0.26 -0.3 4,720,192 - AO353_21565 0.26 -1.0 4,720,192 - AO353_21565 0.26 +0.5 4,720,192 - AO353_21565 0.26 +1.0 4,720,194 + AO353_21565 0.26 -2.5 4,720,195 - AO353_21565 0.27 +1.1 4,720,273 + AO353_21565 0.32 -1.0 4,720,374 + AO353_21565 0.40 -1.6 4,720,385 + -0.0 4,720,385 + AO353_21565 0.41 -1.0 4,720,386 - AO353_21565 0.41 -1.8 4,720,622 + AO353_21565 0.59 -0.4 4,720,622 + AO353_21565 0.59 +2.3 4,720,622 + AO353_21565 0.59 +0.2 4,720,622 + AO353_21565 0.59 +0.8 4,720,622 + AO353_21565 0.59 -2.0 4,720,622 + AO353_21565 0.59 +0.8 4,720,622 + AO353_21565 0.59 -0.1 4,720,622 + AO353_21565 0.59 +0.2 4,720,622 + AO353_21565 0.59 +1.0 4,720,622 + AO353_21565 0.59 +1.9 4,720,622 + AO353_21565 0.59 -1.4 4,720,623 - AO353_21565 0.59 +2.6 4,720,623 - AO353_21565 0.59 -0.8 4,720,623 - AO353_21565 0.59 -0.8 4,720,623 - AO353_21565 0.59 -1.0 4,720,623 - AO353_21565 0.59 -1.5 4,720,623 - AO353_21565 0.59 +3.0 4,720,623 - AO353_21565 0.59 -0.6 4,720,623 - AO353_21565 0.59 +1.6 4,720,623 - AO353_21565 0.59 +1.7 4,720,725 - AO353_21565 0.66 +1.9 4,720,734 + AO353_21565 0.67 -0.9 4,720,735 - AO353_21565 0.67 -1.0 4,721,015 + AO353_21565 0.88 +0.2 4,721,015 + AO353_21565 0.88 +0.2 4,721,033 - AO353_21565 0.89 +1.5 4,721,432 + AO353_21570 0.31 -2.0 4,721,432 + AO353_21570 0.31 +1.4 4,721,433 - AO353_21570 0.31 +1.7 4,721,433 - AO353_21570 0.31 -1.2 4,721,511 + AO353_21570 0.42 -0.5 4,721,525 + AO353_21570 0.43 -1.1 4,721,525 + AO353_21570 0.43 -0.8 4,721,525 + -0.3 4,721,525 + AO353_21570 0.43 -1.0 4,721,526 - AO353_21570 0.43 -0.2 4,721,526 - AO353_21570 0.43 +0.3 4,721,526 - AO353_21570 0.43 -0.7 4,721,526 - AO353_21570 0.43 -0.6 4,721,618 + AO353_21570 0.55 +0.6 4,721,618 + AO353_21570 0.55 -1.3 4,721,618 + AO353_21570 0.55 +0.8 4,721,618 + AO353_21570 0.55 +0.5 4,721,634 + AO353_21570 0.58 -1.8 4,722,070 + -0.7
Or see this region's nucleotide sequence