Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_19275

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
4,223,851	+				-1.7
4,223,851	+				+0.7
4,223,851	+				-1.1
4,223,852	-				-0.4
4,223,852	-				-0.7
4,223,852	-				-0.2
4,223,852	-				-0.3
4,223,852	-				+0.7
4,223,869	+				-2.0
4,223,869	+				-0.1
4,223,870	-				-0.7
4,223,870	-				-3.5
4,223,870	-				+0.4
4,223,870	-				-1.0
4,223,870	-				-1.6
4,223,870	-				+0.2
4,223,870	-				-1.3
4,223,870	-				-1.1
4,223,892	+				+0.3
4,223,892	+				-2.5
4,223,892	+				-1.0
4,223,905	+				-2.2
4,223,906	-				+0.6
4,223,906	-				-0.1
4,223,906	-				-1.9
4,223,947	-				-1.0
4,223,947	-				+2.3
4,223,947	-				-0.6
4,223,947	-				-2.8
4,223,947	-				-0.6
4,223,965	+				-1.4
4,223,965	+				-1.5
4,223,966	-				-1.7
4,223,966	-				-2.2
4,223,966	-				+1.0
4,223,966	-				-0.5
4,223,966	-				-0.7
4,223,966	-				-0.3
4,223,966	-				-1.3
4,223,966	-				-0.2
4,223,995	+				-0.4
4,223,995	+				+0.2
4,223,995	+				-0.1
4,223,995	+				-3.0
4,223,996	-				-0.6
4,223,996	-				-1.1
4,223,996	-				-1.8
4,223,996	-				+0.7
4,223,996	-				-1.6
4,224,012	-				-2.7
4,224,012	-				-0.8
4,224,012	-				-0.7
4,224,012	-				-1.8
4,224,030	+				-1.1
4,224,030	+				-0.8
4,224,030	+				-2.2
4,224,030	+				-2.4
4,224,030	+				-2.7
4,224,030	+				+0.2
4,224,030	+				-0.6
4,224,030	+				-1.9
4,224,030	+				+1.2
4,224,031	-				+0.4
4,224,031	-				-0.4
4,224,031	-				+0.7
4,224,031	-				-2.0
4,224,031	-				-1.6
4,224,031	-				+1.8
4,224,056	+				-0.6
4,224,084	+				-1.2
4,224,084	+				-1.7
4,224,084	+				-0.6
4,224,085	-				-2.4
4,224,125	+				-1.8
4,224,126	-				-2.0
4,224,263	+		AO353_19270	0.27	-1.8
4,224,283	+		AO353_19270	0.30	-1.1
4,224,283	+		AO353_19270	0.30	+0.5
4,224,369	+		AO353_19270	0.43	-0.7
4,224,370	-		AO353_19270	0.43	-2.8
4,224,438	+		AO353_19270	0.54	+0.4
4,224,438	+		AO353_19270	0.54	-2.8
4,224,439	-		AO353_19270	0.54	-1.6
4,224,575	+		AO353_19270	0.75	-1.5
4,224,575	+				+1.2
4,224,575	+		AO353_19270	0.75	-0.7
4,224,575	+		AO353_19270	0.75	-2.8
4,224,575	+		AO353_19270	0.75	-3.4
4,224,575	+		AO353_19270	0.75	-1.2
4,224,575	+		AO353_19270	0.75	-1.4
4,224,575	+				+3.7
4,224,575	+		AO353_19270	0.75	-1.7
4,224,575	+		AO353_19270	0.75	-0.8
4,224,575	+		AO353_19270	0.75	+1.0
4,224,576	-		AO353_19270	0.75	-2.6
4,224,576	-		AO353_19270	0.75	-2.0
4,224,576	-		AO353_19270	0.75	-0.6
4,224,576	-		AO353_19270	0.75	+1.7
4,224,576	-		AO353_19270	0.75	-2.5
4,224,577	+		AO353_19270	0.75	-2.5
4,224,577	+		AO353_19270	0.75	+1.8
4,224,577	+		AO353_19270	0.75	-0.3
4,224,578	-		AO353_19270	0.75	-1.8
4,224,602	+		AO353_19270	0.79	-0.4
4,224,602	+		AO353_19270	0.79	+0.4
4,224,602	+		AO353_19270	0.79	-1.3
4,224,603	-		AO353_19270	0.79	+2.0
4,224,603	-		AO353_19270	0.79	+3.0
4,224,733	+				-0.4
4,224,734	-				-3.0
4,224,734	-				-2.1
4,224,734	-				+0.5
4,224,734	-				-1.1
4,224,753	+				-1.8
4,224,754	-				-0.1
4,224,754	-				+2.8
4,224,760	-				+4.4
4,224,948	+		AO353_19275	0.23	-2.7
4,224,949	-		AO353_19275	0.23	-1.0
4,225,020	+		AO353_19275	0.31	-0.9
4,225,020	+		AO353_19275	0.31	-0.3
4,225,020	+		AO353_19275	0.31	-0.6
4,225,021	-		AO353_19275	0.32	-1.0
4,225,021	-		AO353_19275	0.32	-2.0
4,225,021	-		AO353_19275	0.32	+1.0
4,225,021	-		AO353_19275	0.32	-1.1
4,225,021	-		AO353_19275	0.32	-0.1
4,225,021	-		AO353_19275	0.32	-1.6
4,225,123	-		AO353_19275	0.43	-2.3
4,225,385	-		AO353_19275	0.72	-2.2
4,225,523	+		AO353_19275	0.88	+0.1
4,225,523	+		AO353_19275	0.88	-0.6
4,225,523	+		AO353_19275	0.88	-1.2
4,225,524	-		AO353_19275	0.88	-1.7
4,225,628	+				-2.3
4,225,628	+				-2.1
4,225,628	+				-1.1
4,225,628	+				-0.9
4,225,628	+				-1.4
4,225,628	+				-0.3
4,225,628	+				-0.7
4,225,628	+				-1.2
4,225,629	-				-1.4
4,225,629	-				-0.7
4,225,629	-				-1.0
4,225,629	-				-2.7
4,225,629	-				-1.2
4,225,629	-				+1.6
4,225,629	-				+2.5
4,225,629	-				-0.1
4,225,629	-				-2.7
4,225,633	-				+1.7
4,225,633	-				-2.0
4,225,633	-				+0.1
4,225,633	-				+1.5
4,225,712	-				+2.7
4,225,712	-				-0.2
4,225,719	+				-1.4
4,225,719	+				-1.2
4,225,720	-				-1.7
4,225,720	-				+7.8
4,225,735	-				+5.0
4,225,771	+				-3.0
4,225,771	+				+0.5
4,225,771	+				-1.7
4,225,771	+				-0.3
4,225,772	-				+5.3
4,225,772	-				+1.5
4,225,772	-				+2.7
4,225,772	-				+3.1
4,225,772	-				+3.7
4,225,772	-				+5.5
4,225,823	+				-0.7
4,225,824	-				+5.0
4,225,824	-				+2.7
4,225,837	-				+5.5
4,225,840	-				+5.4
4,225,846	+				+1.6
4,225,846	+				-1.2
4,225,846	+				+1.8
4,225,846	+				-1.2
4,225,846	+				+0.2
4,225,846	+				-1.0
4,225,846	+				-1.7
4,225,846	+				-0.1
4,225,846	+				-0.3
4,225,846	+				-2.2
4,225,847	-				+5.4
4,225,847	-				+3.4
4,225,847	-				+6.1
4,225,847	-				+5.3
4,225,847	-				+5.8
4,225,847	-				+5.0
4,225,852	-				+0.1
4,225,870	+				-0.3
4,225,870	+				-3.0
4,225,871	-				+1.1
4,225,871	-				+2.7
4,225,872	+				-1.3
4,225,872	+				-0.3
4,225,872	+				-1.4
4,225,872	+				-2.6
4,225,872	+				-1.8
4,225,872	+				-1.9
4,225,872	+				-1.1
4,225,872	+				-3.1
4,225,872	+				+0.7
4,225,872	+				-1.9
4,225,872	+				-1.9
4,225,872	+				-2.1
4,225,872	+				-0.7
4,225,872	+				+0.8
4,225,872	+				+0.4
4,225,873	-				+5.3
4,225,873	-				+4.0
4,225,873	-				+4.4
4,225,873	-				+4.9
4,225,873	-				+5.1
4,225,873	-				+6.1
4,225,873	-				+5.7
4,225,873	-				+5.9
4,225,873	-				-1.0
4,225,873	-				+3.8
4,225,873	-				+4.8
4,225,873	-				-0.4
4,225,873	-				+4.0
4,225,873	-				+5.9
4,225,874	+				-0.4
4,225,874	+				-0.7
4,225,874	+				-0.7
4,225,874	+				-2.3
4,225,874	+				-0.2
4,225,874	+				-0.7
4,225,874	+				+0.3
4,225,874	+				-1.1
4,225,874	+				-0.3
4,225,874	+				-1.4
4,225,874	+				-1.2
4,225,874	+				-0.3
4,225,875	-				+6.3
4,225,875	-				+5.5
4,225,875	-				+5.7
4,225,875	-				+4.0
4,225,875	-				+4.2
4,225,875	-				+2.6
4,225,875	-				+6.1
4,225,875	-				+5.3
4,225,875	-				+3.0
4,225,912	+				-2.7
4,225,912	+				-2.2
4,225,912	+				-2.9
4,225,912	+				-1.8
4,225,912	+				+2.2
4,225,912	+				+0.1
4,225,912	+				-0.7
4,225,912	+				-1.1
4,225,912	+				-3.3
4,225,912	+				+1.5
4,225,912	+				-0.9
4,225,912	+				-1.4
4,225,912	+				+1.1
4,225,912	+				-2.3
4,225,912	+				-1.3
4,225,912	+				-1.4
4,225,912	+				-1.9
4,225,913	-				+5.3
4,225,913	-				+5.2
4,225,913	-				+8.3
4,225,913	-				+4.4
4,225,913	-				+2.9
4,225,913	-				+4.5
4,225,913	-				+4.2
4,225,913	-				+4.8
4,225,966	+				-2.7
4,225,966	+				+3.9
4,225,966	+				+0.8
4,225,967	-				+3.6
4,225,967	-				+4.5
4,225,974	+				-2.1
4,226,028	+				-2.8
4,226,029	-				+4.7
4,226,040	+				-0.9
4,226,043	+				-1.4
4,226,060	+				-2.2
4,226,067	+				+0.2
4,226,068	-				+1.8
4,226,075	-				-0.9
4,226,075	-				+3.2
4,226,113	+				-0.3
4,226,114	-				+1.8
4,226,140	+				-2.4
4,226,140	+				-1.9
4,226,258	+		AO353_19280	0.22	-1.9
4,226,258	+		AO353_19280	0.22	+1.1
4,226,258	+		AO353_19280	0.22	-1.3
4,226,258	+		AO353_19280	0.22	-1.4
4,226,259	-		AO353_19280	0.23	-1.9
4,226,608	+				-1.1