Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_15855

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_15840 and AO353_15845 are separated by 34 nucleotidesAO353_15845 and AO353_15850 are separated by 8 nucleotidesAO353_15850 and AO353_15855 are separated by 51 nucleotidesAO353_15855 and AO353_15860 are separated by 3 nucleotidesAO353_15860 and AO353_15865 are separated by 14 nucleotidesAO353_15865 and AO353_15870 are separated by 90 nucleotides AO353_15840: AO353_15840 - scaffolding protein, at 3,498,636 to 3,499,022 _15840 AO353_15845: AO353_15845 - iron-sulfur cluster assembly protein, at 3,499,057 to 3,499,380 _15845 AO353_15850: AO353_15850 - co-chaperone HscB, at 3,499,389 to 3,499,910 _15850 AO353_15855: AO353_15855 - chaperone protein HscA, at 3,499,962 to 3,501,824 _15855 AO353_15860: AO353_15860 - 2Fe-2S ferredoxin, at 3,501,828 to 3,502,169 _15860 AO353_15865: AO353_15865 - Fe-S assembly protein IscX, at 3,502,184 to 3,502,384 _15865 AO353_15870: AO353_15870 - nucleoside diphosphate kinase, at 3,502,475 to 3,502,900 _15870 Position (kb) 3499 3500 3501 3502Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3499.031 kb on + strandat 3499.031 kb on + strandat 3499.044 kb on + strandat 3502.192 kb on + strandat 3502.192 kb on + strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.193 kb on - strandat 3502.259 kb on + strand, within AO353_15865at 3502.393 kb on + strandat 3502.393 kb on + strandat 3502.393 kb on + strandat 3502.393 kb on + strandat 3502.393 kb on + strandat 3502.393 kb on + strandat 3502.424 kb on + strandat 3502.426 kb on + strandat 3502.426 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,499,031 + -0.8
3,499,031 + -2.1
3,499,044 + -1.2
3,502,192 + -1.1
3,502,192 + -1.0
3,502,193 - +0.2
3,502,193 - -0.6
3,502,193 - -0.4
3,502,193 - +1.8
3,502,193 - -0.9
3,502,193 - +0.1
3,502,193 - -1.1
3,502,193 - -1.1
3,502,193 - +0.1
3,502,193 - +0.4
3,502,193 - +2.9
3,502,193 - +2.5
3,502,259 + AO353_15865 0.37 -1.3
3,502,393 + +2.9
3,502,393 + -0.2
3,502,393 + +1.6
3,502,393 + -2.7
3,502,393 + -0.4
3,502,393 + -0.1
3,502,424 + +2.7
3,502,426 + +1.4
3,502,426 + -0.9

Or see this region's nucleotide sequence