Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_15840 and AO353_15845 are separated by 34 nucleotides AO353_15845 and AO353_15850 are separated by 8 nucleotides AO353_15850 and AO353_15855 are separated by 51 nucleotides AO353_15855 and AO353_15860 are separated by 3 nucleotides AO353_15860 and AO353_15865 are separated by 14 nucleotides AO353_15865 and AO353_15870 are separated by 90 nucleotides
AO353_15840: AO353_15840 - scaffolding protein, at 3,498,636 to 3,499,022
_15840
AO353_15845: AO353_15845 - iron-sulfur cluster assembly protein, at 3,499,057 to 3,499,380
_15845
AO353_15850: AO353_15850 - co-chaperone HscB, at 3,499,389 to 3,499,910
_15850
AO353_15855: AO353_15855 - chaperone protein HscA, at 3,499,962 to 3,501,824
_15855
AO353_15860: AO353_15860 - 2Fe-2S ferredoxin, at 3,501,828 to 3,502,169
_15860
AO353_15865: AO353_15865 - Fe-S assembly protein IscX, at 3,502,184 to 3,502,384
_15865
AO353_15870: AO353_15870 - nucleoside diphosphate kinase, at 3,502,475 to 3,502,900
_15870
Position (kb)
3499
3500
3501
3502 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3499.031 kb on + strand at 3499.031 kb on + strand at 3499.044 kb on + strand at 3502.192 kb on + strand at 3502.192 kb on + strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.259 kb on + strand, within AO353_15865 at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.424 kb on + strand at 3502.426 kb on + strand at 3502.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,499,031 + -0.8 3,499,031 + -2.1 3,499,044 + -1.2 3,502,192 + -1.1 3,502,192 + -1.0 3,502,193 - +0.2 3,502,193 - -0.6 3,502,193 - -0.4 3,502,193 - +1.8 3,502,193 - -0.9 3,502,193 - +0.1 3,502,193 - -1.1 3,502,193 - -1.1 3,502,193 - +0.1 3,502,193 - +0.4 3,502,193 - +2.9 3,502,193 - +2.5 3,502,259 + AO353_15865 0.37 -1.3 3,502,393 + +2.9 3,502,393 + -0.2 3,502,393 + +1.6 3,502,393 + -2.7 3,502,393 + -0.4 3,502,393 + -0.1 3,502,424 + +2.7 3,502,426 + +1.4 3,502,426 + -0.9
Or see this region's nucleotide sequence