Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_15850

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_15835 and AO353_15840 are separated by 94 nucleotidesAO353_15840 and AO353_15845 are separated by 34 nucleotidesAO353_15845 and AO353_15850 are separated by 8 nucleotidesAO353_15850 and AO353_15855 are separated by 51 nucleotides AO353_15835: AO353_15835 - cysteine desulfurase IscS, at 3,497,327 to 3,498,541 _15835 AO353_15840: AO353_15840 - scaffolding protein, at 3,498,636 to 3,499,022 _15840 AO353_15845: AO353_15845 - iron-sulfur cluster assembly protein, at 3,499,057 to 3,499,380 _15845 AO353_15850: AO353_15850 - co-chaperone HscB, at 3,499,389 to 3,499,910 _15850 AO353_15855: AO353_15855 - chaperone protein HscA, at 3,499,962 to 3,501,824 _15855 Position (kb) 3499 3500Strain fitness (log2 ratio) -2 -1 0 1at 3498.547 kb on + strandat 3498.547 kb on + strandat 3498.547 kb on + strandat 3498.547 kb on + strandat 3498.547 kb on + strandat 3499.031 kb on + strandat 3499.031 kb on + strandat 3499.044 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,498,547 + -1.2
3,498,547 + -2.1
3,498,547 + +1.4
3,498,547 + +1.5
3,498,547 + +0.4
3,499,031 + -0.8
3,499,031 + -2.1
3,499,044 + -1.2

Or see this region's nucleotide sequence