Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_14850 and AO353_14855 are separated by 2 nucleotides AO353_14855 and AO353_14860 are separated by 165 nucleotides AO353_14860 and AO353_14865 are separated by 3 nucleotides
AO353_14850: AO353_14850 - uracil transporter, at 3,286,955 to 3,288,229
_14850
AO353_14855: AO353_14855 - uracil phosphoribosyltransferase, at 3,288,232 to 3,288,870
_14855
AO353_14860: AO353_14860 - hypoxanthine-guanine phosphoribosyltransferase, at 3,289,036 to 3,289,593
_14860
AO353_14865: AO353_14865 - cupin, at 3,289,597 to 3,290,070
_14865
Position (kb)
3288
3289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.334 kb on + strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.335 kb on - strand, within AO353_14850 at 3287.373 kb on + strand, within AO353_14850 at 3287.373 kb on + strand, within AO353_14850 at 3287.373 kb on + strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.374 kb on - strand, within AO353_14850 at 3287.383 kb on + strand, within AO353_14850 at 3287.383 kb on + strand, within AO353_14850 at 3287.383 kb on + strand, within AO353_14850 at 3287.383 kb on + strand, within AO353_14850 at 3287.384 kb on - strand, within AO353_14850 at 3287.384 kb on - strand, within AO353_14850 at 3287.384 kb on - strand, within AO353_14850 at 3287.466 kb on + strand, within AO353_14850 at 3287.467 kb on - strand, within AO353_14850 at 3287.510 kb on - strand, within AO353_14850 at 3287.669 kb on + strand, within AO353_14850 at 3287.670 kb on - strand, within AO353_14850 at 3287.720 kb on + strand, within AO353_14850 at 3287.720 kb on + strand, within AO353_14850 at 3287.720 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.938 kb on + strand, within AO353_14850 at 3287.939 kb on - strand, within AO353_14850 at 3287.939 kb on - strand, within AO353_14850 at 3287.939 kb on - strand, within AO353_14850 at 3287.939 kb on - strand, within AO353_14850 at 3287.940 kb on + strand, within AO353_14850 at 3287.940 kb on + strand, within AO353_14850 at 3287.941 kb on - strand, within AO353_14850 at 3287.941 kb on - strand, within AO353_14850 at 3288.131 kb on - strand at 3288.296 kb on + strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.297 kb on - strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.335 kb on + strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.336 kb on - strand, within AO353_14855 at 3288.365 kb on - strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.533 kb on + strand, within AO353_14855 at 3288.534 kb on - strand, within AO353_14855 at 3288.658 kb on - strand, within AO353_14855 at 3288.658 kb on - strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.716 kb on + strand, within AO353_14855 at 3288.717 kb on - strand, within AO353_14855 at 3288.717 kb on - strand, within AO353_14855 at 3288.717 kb on - strand, within AO353_14855 at 3288.717 kb on - strand, within AO353_14855 at 3288.749 kb on + strand, within AO353_14855 at 3288.749 kb on + strand, within AO353_14855 at 3288.749 kb on + strand, within AO353_14855 at 3288.750 kb on - strand, within AO353_14855 at 3288.801 kb on + strand, within AO353_14855 at 3288.820 kb on - strand at 3288.907 kb on + strand at 3288.907 kb on + strand at 3288.907 kb on + strand at 3288.907 kb on + strand at 3288.908 kb on - strand at 3288.908 kb on - strand at 3288.912 kb on + strand at 3288.913 kb on - strand at 3288.913 kb on - strand at 3288.913 kb on - strand at 3288.913 kb on - strand at 3288.913 kb on - strand at 3288.913 kb on - strand at 3288.917 kb on + strand at 3288.917 kb on + strand at 3288.918 kb on - strand at 3288.918 kb on - strand at 3288.936 kb on - strand at 3288.936 kb on - strand at 3288.936 kb on - strand at 3288.937 kb on + strand at 3288.937 kb on + strand at 3288.937 kb on + strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.938 kb on - strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.943 kb on + strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.944 kb on - strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.948 kb on + strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.949 kb on - strand at 3288.990 kb on + strand at 3288.990 kb on + strand at 3288.990 kb on + strand at 3288.991 kb on - strand at 3288.991 kb on - strand at 3289.056 kb on + strand at 3289.090 kb on + strand at 3289.090 kb on + strand at 3289.158 kb on + strand, within AO353_14860 at 3289.249 kb on + strand, within AO353_14860 at 3289.249 kb on + strand, within AO353_14860 at 3289.249 kb on + strand, within AO353_14860 at 3289.250 kb on - strand, within AO353_14860 at 3289.250 kb on - strand, within AO353_14860 at 3289.250 kb on - strand, within AO353_14860 at 3289.516 kb on + strand, within AO353_14860 at 3289.516 kb on + strand, within AO353_14860 at 3289.516 kb on + strand, within AO353_14860 at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.540 kb on + strand at 3289.541 kb on - strand at 3289.541 kb on - strand at 3289.541 kb on - strand at 3289.550 kb on - strand at 3289.573 kb on + strand at 3289.573 kb on + strand at 3289.573 kb on + strand at 3289.573 kb on + strand at 3289.573 kb on + strand at 3289.574 kb on - strand at 3289.574 kb on - strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.591 kb on + strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.592 kb on - strand at 3289.632 kb on + strand at 3289.717 kb on - strand, within AO353_14865 at 3289.744 kb on + strand, within AO353_14865 at 3289.744 kb on + strand, within AO353_14865 at 3289.744 kb on + strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865 at 3289.745 kb on - strand, within AO353_14865
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,287,334 + AO353_14850 0.30 +0.2 3,287,334 + AO353_14850 0.30 +0.9 3,287,334 + AO353_14850 0.30 -1.2 3,287,334 + AO353_14850 0.30 +0.5 3,287,334 + AO353_14850 0.30 +1.6 3,287,334 + AO353_14850 0.30 -1.5 3,287,334 + AO353_14850 0.30 -1.0 3,287,334 + AO353_14850 0.30 -0.8 3,287,334 + AO353_14850 0.30 -1.5 3,287,335 - AO353_14850 0.30 -1.2 3,287,335 - AO353_14850 0.30 -0.1 3,287,335 - AO353_14850 0.30 -1.3 3,287,335 - AO353_14850 0.30 +0.5 3,287,335 - AO353_14850 0.30 -1.4 3,287,335 - AO353_14850 0.30 +0.6 3,287,335 - AO353_14850 0.30 +0.9 3,287,335 - AO353_14850 0.30 +1.2 3,287,335 - AO353_14850 0.30 -0.5 3,287,373 + AO353_14850 0.33 +0.5 3,287,373 + AO353_14850 0.33 -1.2 3,287,373 + AO353_14850 0.33 +0.7 3,287,374 - AO353_14850 0.33 -1.8 3,287,374 - AO353_14850 0.33 -2.1 3,287,374 - AO353_14850 0.33 +0.5 3,287,374 - AO353_14850 0.33 -0.8 3,287,374 - AO353_14850 0.33 +1.4 3,287,374 - AO353_14850 0.33 +0.4 3,287,374 - AO353_14850 0.33 -1.2 3,287,383 + AO353_14850 0.34 -1.1 3,287,383 + AO353_14850 0.34 -1.9 3,287,383 + AO353_14850 0.34 -0.9 3,287,383 + AO353_14850 0.34 -0.6 3,287,384 - AO353_14850 0.34 +1.9 3,287,384 - AO353_14850 0.34 -0.2 3,287,384 - AO353_14850 0.34 +0.5 3,287,466 + AO353_14850 0.40 +1.4 3,287,467 - AO353_14850 0.40 -1.2 3,287,510 - AO353_14850 0.44 +0.6 3,287,669 + AO353_14850 0.56 -1.9 3,287,670 - AO353_14850 0.56 +1.7 3,287,720 + AO353_14850 0.60 -1.5 3,287,720 + AO353_14850 0.60 +0.2 3,287,720 + AO353_14850 0.60 -1.5 3,287,938 + AO353_14850 0.77 +1.3 3,287,938 + AO353_14850 0.77 +0.5 3,287,938 + AO353_14850 0.77 -0.3 3,287,938 + AO353_14850 0.77 +1.7 3,287,938 + AO353_14850 0.77 +1.6 3,287,938 + AO353_14850 0.77 -1.4 3,287,939 - AO353_14850 0.77 -0.3 3,287,939 - AO353_14850 0.77 +0.8 3,287,939 - AO353_14850 0.77 -2.0 3,287,939 - AO353_14850 0.77 -2.4 3,287,940 + AO353_14850 0.77 -0.9 3,287,940 + AO353_14850 0.77 -0.0 3,287,941 - AO353_14850 0.77 -0.7 3,287,941 - AO353_14850 0.77 +0.8 3,288,131 - -0.6 3,288,296 + AO353_14855 0.10 -1.1 3,288,297 - AO353_14855 0.10 -2.0 3,288,297 - AO353_14855 0.10 -0.2 3,288,297 - AO353_14855 0.10 +1.8 3,288,297 - AO353_14855 0.10 +1.5 3,288,297 - AO353_14855 0.10 -1.3 3,288,297 - AO353_14855 0.10 -0.8 3,288,335 + AO353_14855 0.16 +0.3 3,288,335 + AO353_14855 0.16 +0.7 3,288,335 + AO353_14855 0.16 +1.5 3,288,335 + AO353_14855 0.16 +1.1 3,288,335 + AO353_14855 0.16 -0.2 3,288,335 + AO353_14855 0.16 -0.7 3,288,336 - AO353_14855 0.16 -0.6 3,288,336 - AO353_14855 0.16 -1.9 3,288,336 - AO353_14855 0.16 -0.3 3,288,336 - AO353_14855 0.16 +0.5 3,288,336 - AO353_14855 0.16 +1.4 3,288,336 - AO353_14855 0.16 +0.9 3,288,336 - AO353_14855 0.16 +0.2 3,288,336 - AO353_14855 0.16 -0.0 3,288,336 - AO353_14855 0.16 -0.9 3,288,336 - AO353_14855 0.16 +0.5 3,288,336 - AO353_14855 0.16 -0.5 3,288,365 - AO353_14855 0.21 +0.6 3,288,533 + AO353_14855 0.47 +0.8 3,288,533 + AO353_14855 0.47 +1.2 3,288,533 + AO353_14855 0.47 -2.3 3,288,533 + AO353_14855 0.47 -1.3 3,288,533 + AO353_14855 0.47 -0.6 3,288,533 + AO353_14855 0.47 +0.7 3,288,533 + AO353_14855 0.47 +1.8 3,288,533 + AO353_14855 0.47 -0.8 3,288,533 + AO353_14855 0.47 -1.0 3,288,533 + AO353_14855 0.47 +1.2 3,288,533 + AO353_14855 0.47 -1.4 3,288,533 + AO353_14855 0.47 +2.6 3,288,534 - AO353_14855 0.47 -0.2 3,288,658 - AO353_14855 0.67 +2.9 3,288,658 - AO353_14855 0.67 +2.1 3,288,716 + AO353_14855 0.76 -2.0 3,288,716 + AO353_14855 0.76 +0.2 3,288,716 + AO353_14855 0.76 +2.8 3,288,716 + AO353_14855 0.76 +1.3 3,288,716 + AO353_14855 0.76 +2.2 3,288,716 + AO353_14855 0.76 +2.1 3,288,716 + AO353_14855 0.76 +0.2 3,288,716 + AO353_14855 0.76 -0.3 3,288,716 + AO353_14855 0.76 +0.5 3,288,717 - AO353_14855 0.76 +0.2 3,288,717 - AO353_14855 0.76 -0.6 3,288,717 - AO353_14855 0.76 +0.9 3,288,717 - AO353_14855 0.76 -2.6 3,288,749 + AO353_14855 0.81 +1.2 3,288,749 + AO353_14855 0.81 -0.2 3,288,749 + AO353_14855 0.81 -2.4 3,288,750 - AO353_14855 0.81 +1.3 3,288,801 + AO353_14855 0.89 -0.4 3,288,820 - -0.9 3,288,907 + +0.2 3,288,907 + +3.8 3,288,907 + -0.7 3,288,907 + +2.9 3,288,908 - +0.1 3,288,908 - -0.6 3,288,912 + -0.2 3,288,913 - +1.6 3,288,913 - -1.0 3,288,913 - +3.3 3,288,913 - -0.2 3,288,913 - -1.8 3,288,913 - +0.9 3,288,917 + +0.2 3,288,917 + -1.0 3,288,918 - -0.5 3,288,918 - +2.1 3,288,936 - +0.4 3,288,936 - -0.3 3,288,936 - +1.8 3,288,937 + +0.6 3,288,937 + -1.5 3,288,937 + +0.4 3,288,938 - -1.1 3,288,938 - -1.5 3,288,938 - +0.8 3,288,938 - -1.3 3,288,938 - -2.1 3,288,938 - -2.5 3,288,938 - +0.4 3,288,938 - +1.1 3,288,943 + +0.8 3,288,943 + -0.2 3,288,943 + -0.3 3,288,943 + -1.3 3,288,943 + -0.7 3,288,943 + +1.0 3,288,943 + -0.2 3,288,943 + +0.1 3,288,943 + +2.2 3,288,943 + -0.2 3,288,943 + -0.4 3,288,943 + -0.4 3,288,943 + -1.1 3,288,943 + +2.1 3,288,943 + -0.5 3,288,943 + +2.7 3,288,943 + -0.4 3,288,943 + +0.7 3,288,943 + +0.9 3,288,943 + -0.9 3,288,943 + +0.9 3,288,943 + +1.0 3,288,943 + -0.5 3,288,943 + -0.8 3,288,944 - -0.2 3,288,944 - -3.2 3,288,944 - -1.3 3,288,944 - +0.5 3,288,944 - -0.6 3,288,944 - -1.4 3,288,944 - -0.9 3,288,944 - +2.2 3,288,944 - +0.6 3,288,944 - -0.3 3,288,944 - +1.9 3,288,944 - -1.4 3,288,944 - -1.9 3,288,944 - -1.0 3,288,944 - -0.7 3,288,944 - +1.1 3,288,944 - +0.7 3,288,944 - -1.1 3,288,944 - +0.4 3,288,948 + +1.9 3,288,948 + -1.6 3,288,948 + +0.3 3,288,948 + +0.7 3,288,948 + +0.2 3,288,948 + -0.7 3,288,948 + +1.6 3,288,948 + -0.0 3,288,948 + +0.5 3,288,949 - +0.2 3,288,949 - -1.3 3,288,949 - -0.2 3,288,949 - -0.0 3,288,949 - -1.6 3,288,949 - +0.3 3,288,949 - -0.2 3,288,949 - +2.6 3,288,949 - +1.0 3,288,990 + +1.0 3,288,990 + +0.5 3,288,990 + +2.6 3,288,991 - +0.2 3,288,991 - +0.3 3,289,056 + -1.7 3,289,090 + +2.0 3,289,090 + +1.3 3,289,158 + AO353_14860 0.22 +0.5 3,289,249 + AO353_14860 0.38 +1.6 3,289,249 + AO353_14860 0.38 +1.0 3,289,249 + AO353_14860 0.38 -1.0 3,289,250 - AO353_14860 0.38 -0.0 3,289,250 - AO353_14860 0.38 -0.5 3,289,250 - AO353_14860 0.38 -0.2 3,289,516 + AO353_14860 0.86 +0.5 3,289,516 + AO353_14860 0.86 -1.4 3,289,516 + AO353_14860 0.86 -2.4 3,289,540 + +0.2 3,289,540 + -0.6 3,289,540 + +1.1 3,289,540 + +2.2 3,289,540 + +0.8 3,289,540 + -0.5 3,289,540 + -0.0 3,289,540 + -2.4 3,289,540 + -0.9 3,289,541 - -1.4 3,289,541 - -0.7 3,289,541 - +0.2 3,289,550 - -1.5 3,289,573 + +1.1 3,289,573 + -1.4 3,289,573 + +0.7 3,289,573 + -1.8 3,289,573 + -1.5 3,289,574 - -2.0 3,289,574 - -0.5 3,289,591 + +0.2 3,289,591 + +1.6 3,289,591 + +0.9 3,289,591 + -1.3 3,289,591 + -2.0 3,289,591 + +0.2 3,289,591 + -2.3 3,289,591 + +0.4 3,289,591 + +1.4 3,289,591 + +0.1 3,289,591 + +2.2 3,289,591 + -0.5 3,289,591 + -2.0 3,289,591 + +0.4 3,289,592 - +0.9 3,289,592 - +0.4 3,289,592 - +0.8 3,289,592 - -1.6 3,289,592 - +1.4 3,289,592 - -1.1 3,289,592 - -1.0 3,289,592 - -0.6 3,289,632 + +1.1 3,289,717 - AO353_14865 0.25 -0.9 3,289,744 + AO353_14865 0.31 +1.1 3,289,744 + AO353_14865 0.31 +3.7 3,289,744 + AO353_14865 0.31 -2.0 3,289,745 - AO353_14865 0.31 -2.0 3,289,745 - AO353_14865 0.31 -1.0 3,289,745 - AO353_14865 0.31 +2.3 3,289,745 - AO353_14865 0.31 -0.6 3,289,745 - AO353_14865 0.31 -1.4 3,289,745 - AO353_14865 0.31 -0.2
Or see this region's nucleotide sequence