Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_08365 and AO353_08370 are separated by 132 nucleotides AO353_08370 and AO353_08375 are separated by 85 nucleotides
AO353_08365: AO353_08365 - L-cystine transporter tcyP, at 1,839,326 to 1,840,717
_08365
AO353_08370: AO353_08370 - diacylglycerol kinase, at 1,840,850 to 1,841,362
_08370
AO353_08375: AO353_08375 - hypothetical protein, at 1,841,448 to 1,842,827
_08375
Position (kb)
1840
1841
1842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1840.047 kb on + strand, within AO353_08365 at 1840.047 kb on + strand, within AO353_08365 at 1840.127 kb on + strand, within AO353_08365 at 1840.127 kb on + strand, within AO353_08365 at 1840.127 kb on + strand, within AO353_08365 at 1840.127 kb on + strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.128 kb on - strand, within AO353_08365 at 1840.301 kb on - strand, within AO353_08365 at 1840.345 kb on + strand, within AO353_08365 at 1840.345 kb on + strand, within AO353_08365 at 1840.346 kb on - strand, within AO353_08365 at 1840.346 kb on - strand, within AO353_08365 at 1840.346 kb on - strand, within AO353_08365 at 1840.643 kb on - strand at 1840.715 kb on + strand at 1840.715 kb on + strand at 1840.715 kb on + strand at 1840.716 kb on - strand at 1840.716 kb on - strand at 1840.716 kb on - strand at 1840.725 kb on - strand at 1840.725 kb on - strand at 1840.734 kb on + strand at 1840.734 kb on + strand at 1840.735 kb on - strand at 1840.735 kb on - strand at 1840.735 kb on - strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.788 kb on + strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.789 kb on - strand at 1840.810 kb on + strand at 1840.811 kb on - strand at 1840.811 kb on - strand at 1840.811 kb on - strand at 1840.851 kb on + strand at 1840.851 kb on + strand at 1840.851 kb on + strand at 1840.852 kb on - strand at 1841.375 kb on - strand at 1841.413 kb on + strand at 1841.683 kb on + strand, within AO353_08375 at 1841.821 kb on + strand, within AO353_08375 at 1841.822 kb on - strand, within AO353_08375 at 1841.822 kb on - strand, within AO353_08375 at 1841.835 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.857 kb on + strand, within AO353_08375 at 1841.858 kb on - strand, within AO353_08375 at 1841.858 kb on - strand, within AO353_08375 at 1841.858 kb on - strand, within AO353_08375 at 1841.858 kb on - strand, within AO353_08375 at 1841.858 kb on - strand, within AO353_08375 at 1842.243 kb on + strand, within AO353_08375 at 1842.243 kb on + strand, within AO353_08375 at 1842.339 kb on + strand, within AO353_08375
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,840,047 + AO353_08365 0.52 +0.1 1,840,047 + AO353_08365 0.52 -1.8 1,840,127 + AO353_08365 0.58 -2.3 1,840,127 + AO353_08365 0.58 +2.9 1,840,127 + AO353_08365 0.58 +1.4 1,840,127 + AO353_08365 0.58 +3.5 1,840,128 - AO353_08365 0.58 +0.5 1,840,128 - AO353_08365 0.58 +0.2 1,840,128 - AO353_08365 0.58 +1.0 1,840,128 - AO353_08365 0.58 +1.0 1,840,128 - AO353_08365 0.58 +2.2 1,840,128 - AO353_08365 0.58 +0.4 1,840,301 - AO353_08365 0.70 -1.4 1,840,345 + AO353_08365 0.73 -1.6 1,840,345 + AO353_08365 0.73 +1.8 1,840,346 - AO353_08365 0.73 +0.4 1,840,346 - AO353_08365 0.73 +3.2 1,840,346 - AO353_08365 0.73 -1.5 1,840,643 - -0.0 1,840,715 + -0.8 1,840,715 + -0.6 1,840,715 + -1.0 1,840,716 - +2.1 1,840,716 - -1.1 1,840,716 - -1.0 1,840,725 - -2.2 1,840,725 - -1.2 1,840,734 + -1.5 1,840,734 + -1.5 1,840,735 - +3.1 1,840,735 - -0.1 1,840,735 - +0.4 1,840,788 + +2.9 1,840,788 + +0.4 1,840,788 + +0.1 1,840,788 + -0.9 1,840,788 + +2.1 1,840,788 + -0.7 1,840,788 + +2.8 1,840,788 + +1.9 1,840,788 + +0.5 1,840,788 + -3.1 1,840,789 - +0.6 1,840,789 - +2.0 1,840,789 - +0.6 1,840,789 - +0.8 1,840,789 - -1.9 1,840,789 - -1.2 1,840,789 - -1.8 1,840,789 - -1.8 1,840,789 - +2.2 1,840,810 + -0.5 1,840,811 - -0.7 1,840,811 - +2.7 1,840,811 - -2.0 1,840,851 + -0.6 1,840,851 + +1.3 1,840,851 + -0.4 1,840,852 - +1.6 1,841,375 - -0.9 1,841,413 + -1.1 1,841,683 + AO353_08375 0.17 -0.0 1,841,821 + AO353_08375 0.27 -0.7 1,841,822 - AO353_08375 0.27 -1.5 1,841,822 - AO353_08375 0.27 -1.1 1,841,835 + AO353_08375 0.28 +1.6 1,841,857 + AO353_08375 0.30 +1.2 1,841,857 + AO353_08375 0.30 -0.2 1,841,857 + AO353_08375 0.30 -0.5 1,841,857 + AO353_08375 0.30 -0.2 1,841,857 + AO353_08375 0.30 -1.6 1,841,857 + AO353_08375 0.30 -0.6 1,841,857 + AO353_08375 0.30 -0.7 1,841,857 + AO353_08375 0.30 +0.8 1,841,857 + AO353_08375 0.30 +1.1 1,841,858 - AO353_08375 0.30 -1.8 1,841,858 - AO353_08375 0.30 +1.7 1,841,858 - AO353_08375 0.30 +0.8 1,841,858 - AO353_08375 0.30 +1.0 1,841,858 - AO353_08375 0.30 -0.9 1,842,243 + AO353_08375 0.58 -0.9 1,842,243 + AO353_08375 0.58 +2.2 1,842,339 + AO353_08375 0.65 -0.4
Or see this region's nucleotide sequence