Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_08115 and AO353_08120 are separated by 240 nucleotides AO353_08120 and AO353_08125 overlap by 1 nucleotides AO353_08125 and AO353_08130 are separated by 95 nucleotides AO353_08130 and AO353_08135 are separated by 26 nucleotides
AO353_08115: AO353_08115 - energy transducer TonB, at 1,791,856 to 1,792,755
_08115
AO353_08120: AO353_08120 - hypothetical protein, at 1,792,996 to 1,793,565
_08120
AO353_08125: AO353_08125 - Holliday junction resolvase, at 1,793,565 to 1,794,002
_08125
AO353_08130: AO353_08130 - uracil phosphoribosyltransferase, at 1,794,098 to 1,794,604
_08130
AO353_08135: AO353_08135 - aspartate carbamoyltransferase catalytic subunit, at 1,794,631 to 1,795,635
_08135
Position (kb) 1793
1794
1795 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1792.579 kb on + strand, within AO353_08115 at 1792.580 kb on - strand, within AO353_08115 at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.868 kb on - strand at 1792.868 kb on - strand at 1792.868 kb on - strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1794.043 kb on + strand at 1794.043 kb on + strand at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.284 kb on + strand, within AO353_08130 at 1794.284 kb on + strand, within AO353_08130
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,792,579 + AO353_08115 0.80 -1.0 1,792,580 - AO353_08115 0.80 -1.2 1,792,749 + -2.9 1,792,749 + +0.1 1,792,749 + +0.1 1,792,749 + -0.7 1,792,868 - -2.3 1,792,868 - -1.6 1,792,868 - +0.7 1,793,017 + -0.5 1,793,017 + -0.7 1,793,017 + +0.3 1,793,017 + +1.2 1,793,086 + AO353_08120 0.16 +0.4 1,793,086 + AO353_08120 0.16 +0.2 1,793,086 + AO353_08120 0.16 -0.8 1,793,086 + AO353_08120 0.16 +0.7 1,793,086 + AO353_08120 0.16 -0.4 1,793,086 + AO353_08120 0.16 +0.0 1,793,086 + AO353_08120 0.16 -1.6 1,793,086 + AO353_08120 0.16 -1.0 1,793,086 + AO353_08120 0.16 +2.4 1,793,281 + AO353_08120 0.50 -0.5 1,793,281 + AO353_08120 0.50 +0.4 1,793,281 + AO353_08120 0.50 -0.1 1,794,043 + +1.2 1,794,043 + -1.4 1,794,243 + AO353_08130 0.29 -0.6 1,794,243 + AO353_08130 0.29 -0.1 1,794,243 + AO353_08130 0.29 -1.4 1,794,243 + AO353_08130 0.29 -3.4 1,794,243 + AO353_08130 0.29 +2.2 1,794,284 + AO353_08130 0.37 -0.9 1,794,284 + AO353_08130 0.37 -0.8
Or see this region's nucleotide sequence