Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02870 and AO353_02875 are separated by 81 nucleotides AO353_02875 and AO353_02880 are separated by 151 nucleotides AO353_02880 and AO353_02885 are separated by 48 nucleotides AO353_02885 and AO353_02890 are separated by 95 nucleotides
AO353_02870: AO353_02870 - chemotaxis protein CheW, at 610,044 to 610,976
_02870
AO353_02875: AO353_02875 - flagellar biosynthesis protein FlgA, at 611,058 to 611,819
_02875
AO353_02880: AO353_02880 - flagellar biosynthesis anti-sigma factor FlgM, at 611,971 to 612,297
_02880
AO353_02885: AO353_02885 - flagellar biosynthesis protein FlgN, at 612,346 to 612,813
_02885
AO353_02890: AO353_02890 - pilus assembly protein PilZ, at 612,909 to 613,655
_02890
Position (kb) 611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 610.988 kb on + strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.989 kb on - strand at 610.999 kb on + strand at 610.999 kb on + strand at 610.999 kb on + strand at 610.999 kb on + strand at 610.999 kb on + strand at 610.999 kb on + strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.000 kb on - strand at 611.028 kb on - strand at 611.051 kb on - strand at 611.051 kb on - strand at 611.105 kb on + strand at 611.131 kb on + strand at 611.131 kb on + strand at 611.131 kb on + strand at 611.132 kb on - strand at 611.174 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.188 kb on + strand, within AO353_02875 at 611.189 kb on - strand, within AO353_02875 at 611.189 kb on - strand, within AO353_02875 at 611.189 kb on - strand, within AO353_02875 at 611.189 kb on - strand, within AO353_02875 at 611.189 kb on - strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.227 kb on + strand, within AO353_02875 at 611.228 kb on - strand, within AO353_02875 at 611.228 kb on - strand, within AO353_02875 at 611.235 kb on + strand, within AO353_02875 at 611.235 kb on + strand, within AO353_02875 at 611.235 kb on + strand, within AO353_02875 at 611.236 kb on - strand, within AO353_02875 at 611.236 kb on - strand, within AO353_02875 at 611.236 kb on - strand, within AO353_02875 at 611.351 kb on + strand, within AO353_02875 at 611.352 kb on - strand, within AO353_02875 at 611.353 kb on + strand, within AO353_02875 at 611.353 kb on + strand, within AO353_02875 at 611.353 kb on + strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.354 kb on - strand, within AO353_02875 at 611.541 kb on + strand, within AO353_02875 at 611.609 kb on + strand, within AO353_02875 at 611.610 kb on - strand, within AO353_02875 at 611.610 kb on - strand, within AO353_02875 at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.817 kb on + strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.818 kb on - strand at 611.821 kb on + strand at 611.821 kb on + strand at 611.821 kb on + strand at 611.821 kb on + strand at 611.822 kb on - strand at 611.822 kb on - strand at 611.822 kb on - strand at 611.822 kb on - strand at 611.822 kb on - strand at 611.822 kb on - strand at 611.848 kb on + strand at 611.873 kb on + strand at 611.873 kb on + strand at 611.873 kb on + strand at 611.899 kb on - strand at 611.899 kb on - strand at 611.907 kb on + strand at 611.907 kb on + strand at 611.907 kb on + strand at 611.908 kb on - strand at 611.908 kb on - strand at 611.993 kb on + strand at 612.238 kb on + strand, within AO353_02880 at 612.276 kb on + strand at 612.277 kb on - strand at 612.277 kb on - strand at 612.277 kb on - strand at 612.328 kb on + strand at 612.328 kb on + strand at 612.329 kb on - strand at 612.329 kb on - strand at 612.362 kb on + strand at 612.362 kb on + strand at 612.363 kb on - strand at 612.363 kb on - strand at 612.363 kb on - strand at 612.363 kb on - strand at 612.597 kb on + strand, within AO353_02885 at 612.754 kb on + strand, within AO353_02885 at 612.755 kb on - strand, within AO353_02885 at 612.755 kb on - strand, within AO353_02885 at 612.819 kb on + strand at 612.820 kb on - strand at 612.820 kb on - strand at 612.820 kb on - strand at 612.820 kb on - strand at 612.820 kb on - strand at 612.823 kb on + strand at 612.823 kb on + strand at 612.823 kb on + strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.824 kb on - strand at 612.834 kb on + strand at 612.834 kb on + strand at 612.884 kb on + strand at 612.884 kb on + strand at 612.884 kb on + strand at 612.884 kb on + strand at 612.885 kb on - strand at 612.885 kb on - strand at 612.903 kb on + strand at 612.903 kb on + strand at 612.904 kb on - strand at 613.059 kb on + strand, within AO353_02890 at 613.059 kb on + strand, within AO353_02890 at 613.059 kb on + strand, within AO353_02890 at 613.059 kb on + strand, within AO353_02890 at 613.060 kb on - strand, within AO353_02890 at 613.180 kb on + strand, within AO353_02890 at 613.180 kb on + strand, within AO353_02890 at 613.180 kb on + strand, within AO353_02890 at 613.180 kb on + strand, within AO353_02890 at 613.180 kb on + strand, within AO353_02890 at 613.181 kb on - strand, within AO353_02890 at 613.181 kb on - strand, within AO353_02890 at 613.181 kb on - strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.184 kb on + strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.185 kb on - strand, within AO353_02890 at 613.243 kb on - strand, within AO353_02890 at 613.273 kb on - strand, within AO353_02890 at 613.273 kb on - strand, within AO353_02890
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 610,988 + -1.7 610,989 - +3.0 610,989 - +0.5 610,989 - +0.1 610,989 - -0.3 610,989 - +0.8 610,989 - -0.3 610,989 - -1.4 610,989 - -0.9 610,999 + -0.7 610,999 + -0.9 610,999 + +1.7 610,999 + -2.4 610,999 + +0.0 610,999 + -2.1 611,000 - -2.5 611,000 - +1.6 611,000 - +0.6 611,000 - -2.2 611,000 - -2.4 611,000 - -0.7 611,000 - -1.5 611,028 - +0.5 611,051 - -0.9 611,051 - -2.4 611,105 + -0.5 611,131 + -1.1 611,131 + -1.2 611,131 + +0.0 611,132 - -3.4 611,174 + AO353_02875 0.15 -0.9 611,188 + AO353_02875 0.17 -2.3 611,188 + AO353_02875 0.17 -1.4 611,188 + AO353_02875 0.17 -3.0 611,188 + AO353_02875 0.17 -2.8 611,188 + AO353_02875 0.17 -1.5 611,188 + AO353_02875 0.17 -1.3 611,188 + AO353_02875 0.17 -1.8 611,189 - AO353_02875 0.17 -3.4 611,189 - AO353_02875 0.17 -0.4 611,189 - AO353_02875 0.17 -1.8 611,189 - AO353_02875 0.17 -2.7 611,189 - AO353_02875 0.17 -1.9 611,227 + AO353_02875 0.22 -1.1 611,227 + AO353_02875 0.22 +0.5 611,227 + AO353_02875 0.22 -1.6 611,227 + AO353_02875 0.22 -1.5 611,227 + AO353_02875 0.22 -1.5 611,227 + AO353_02875 0.22 +0.0 611,228 - AO353_02875 0.22 -1.3 611,228 - AO353_02875 0.22 +0.5 611,235 + AO353_02875 0.23 -0.2 611,235 + AO353_02875 0.23 -0.3 611,235 + AO353_02875 0.23 +0.5 611,236 - AO353_02875 0.23 -3.2 611,236 - AO353_02875 0.23 -2.5 611,236 - AO353_02875 0.23 -1.6 611,351 + AO353_02875 0.38 -0.8 611,352 - AO353_02875 0.39 -1.6 611,353 + AO353_02875 0.39 -1.4 611,353 + AO353_02875 0.39 -2.1 611,353 + AO353_02875 0.39 -1.9 611,354 - AO353_02875 0.39 -1.7 611,354 - AO353_02875 0.39 -1.1 611,354 - AO353_02875 0.39 -0.5 611,354 - AO353_02875 0.39 -1.8 611,354 - AO353_02875 0.39 -0.8 611,354 - AO353_02875 0.39 -2.4 611,354 - AO353_02875 0.39 -1.0 611,354 - AO353_02875 0.39 -3.1 611,354 - AO353_02875 0.39 -0.8 611,541 + AO353_02875 0.63 +0.0 611,609 + AO353_02875 0.72 -1.3 611,610 - AO353_02875 0.72 -2.7 611,610 - AO353_02875 0.72 -1.4 611,817 + -0.8 611,817 + -1.4 611,817 + +1.3 611,817 + -0.8 611,817 + -0.4 611,817 + -2.1 611,817 + -3.0 611,817 + -0.3 611,817 + -1.1 611,817 + -0.1 611,817 + -1.1 611,817 + -2.9 611,817 + -0.3 611,817 + -1.8 611,818 - -0.4 611,818 - -1.3 611,818 - -1.5 611,818 - +0.4 611,818 - +0.8 611,818 - -1.9 611,818 - -0.5 611,818 - -1.5 611,818 - +0.2 611,818 - -1.9 611,818 - +2.1 611,818 - -0.5 611,821 + -0.1 611,821 + -1.2 611,821 + -1.1 611,821 + -0.9 611,822 - -0.8 611,822 - -1.5 611,822 - -0.7 611,822 - +1.0 611,822 - +0.3 611,822 - -1.4 611,848 + +1.0 611,873 + -0.4 611,873 + -2.4 611,873 + +1.8 611,899 - -2.4 611,899 - +0.4 611,907 + -0.9 611,907 + -0.7 611,907 + -1.2 611,908 - -2.5 611,908 - +0.6 611,993 + +0.8 612,238 + AO353_02880 0.82 -0.9 612,276 + +1.1 612,277 - -3.1 612,277 - -0.6 612,277 - -2.0 612,328 + +2.1 612,328 + +0.7 612,329 - -2.5 612,329 - -0.4 612,362 + -2.7 612,362 + -1.8 612,363 - -1.2 612,363 - -1.8 612,363 - -3.0 612,363 - -0.2 612,597 + AO353_02885 0.54 -2.5 612,754 + AO353_02885 0.87 -2.2 612,755 - AO353_02885 0.87 -2.5 612,755 - AO353_02885 0.87 +0.0 612,819 + -2.9 612,820 - +0.1 612,820 - -0.8 612,820 - -1.8 612,820 - -1.3 612,820 - -1.2 612,823 + -0.6 612,823 + -2.3 612,823 + +2.7 612,824 - -0.1 612,824 - -0.6 612,824 - -0.7 612,824 - +1.7 612,824 - +0.6 612,824 - +0.5 612,824 - -1.6 612,824 - +3.9 612,834 + +3.1 612,834 + -0.2 612,884 + -1.4 612,884 + -0.9 612,884 + +1.2 612,884 + +0.4 612,885 - -0.4 612,885 - -2.5 612,903 + +0.2 612,903 + +1.2 612,904 - -2.6 613,059 + AO353_02890 0.20 -0.7 613,059 + AO353_02890 0.20 +1.6 613,059 + AO353_02890 0.20 +0.0 613,059 + AO353_02890 0.20 -2.0 613,060 - AO353_02890 0.20 -1.1 613,180 + AO353_02890 0.36 -2.0 613,180 + AO353_02890 0.36 -0.5 613,180 + AO353_02890 0.36 -1.3 613,180 + AO353_02890 0.36 -1.9 613,180 + AO353_02890 0.36 -0.8 613,181 - AO353_02890 0.36 -1.5 613,181 - AO353_02890 0.36 -0.4 613,181 - AO353_02890 0.36 -1.7 613,184 + AO353_02890 0.37 -1.1 613,184 + AO353_02890 0.37 -1.4 613,184 + AO353_02890 0.37 +0.5 613,184 + AO353_02890 0.37 -0.9 613,184 + AO353_02890 0.37 +0.2 613,184 + AO353_02890 0.37 +0.3 613,184 + AO353_02890 0.37 -1.6 613,184 + AO353_02890 0.37 +1.2 613,184 + AO353_02890 0.37 -0.6 613,184 + AO353_02890 0.37 -1.2 613,184 + AO353_02890 0.37 -0.2 613,185 - AO353_02890 0.37 -0.4 613,185 - AO353_02890 0.37 -0.4 613,185 - AO353_02890 0.37 +0.0 613,185 - AO353_02890 0.37 +1.4 613,185 - AO353_02890 0.37 +1.3 613,185 - AO353_02890 0.37 -0.4 613,243 - AO353_02890 0.45 -2.6 613,273 - AO353_02890 0.49 +1.6 613,273 - AO353_02890 0.49 -1.1
Or see this region's nucleotide sequence