Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02370 and AO353_02375 are separated by 200 nucleotides AO353_02375 and AO353_02380 overlap by 1 nucleotides AO353_02380 and AO353_02385 are separated by 91 nucleotides
AO353_02370: AO353_02370 - septum formation inhibitor, at 504,254 to 504,991
_02370
AO353_02375: AO353_02375 - septum site-determining protein MinD, at 505,192 to 506,004
_02375
AO353_02380: AO353_02380 - cell division topological specificity factor, at 506,004 to 506,258
_02380
AO353_02385: AO353_02385 - pseudouridine synthase, at 506,350 to 506,985
_02385
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 504.212 kb on + strand at 504.212 kb on + strand at 504.212 kb on + strand at 504.212 kb on + strand at 504.213 kb on - strand at 504.228 kb on + strand at 504.228 kb on + strand at 504.244 kb on + strand at 504.791 kb on + strand, within AO353_02370 at 504.945 kb on + strand at 505.085 kb on + strand at 505.085 kb on + strand at 505.132 kb on + strand at 506.335 kb on + strand at 506.335 kb on + strand at 506.348 kb on + strand at 506.348 kb on + strand at 506.349 kb on - strand at 506.393 kb on + strand at 506.393 kb on + strand at 506.412 kb on + strand at 506.413 kb on - strand at 506.614 kb on + strand, within AO353_02385 at 506.615 kb on - strand, within AO353_02385 at 506.867 kb on - strand, within AO353_02385 at 506.983 kb on + strand at 506.984 kb on - strand at 506.991 kb on - strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.993 kb on + strand at 506.994 kb on - strand at 506.994 kb on - strand at 506.994 kb on - strand at 506.994 kb on - strand at 506.994 kb on - strand at 506.994 kb on - strand at 506.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 504,212 + -1.2 504,212 + -0.2 504,212 + -0.6 504,212 + -0.9 504,213 - -0.7 504,228 + -0.7 504,228 + +0.8 504,244 + -0.1 504,791 + AO353_02370 0.73 +1.4 504,945 + +0.9 505,085 + -2.5 505,085 + +0.1 505,132 + -1.6 506,335 + -1.2 506,335 + +2.1 506,348 + +1.8 506,348 + -2.0 506,349 - -0.4 506,393 + +0.5 506,393 + -3.3 506,412 + -0.1 506,413 - -0.2 506,614 + AO353_02385 0.42 -1.2 506,615 - AO353_02385 0.42 -1.3 506,867 - AO353_02385 0.81 -0.5 506,983 + +0.4 506,984 - +1.5 506,991 - -0.5 506,993 + -3.3 506,993 + -0.6 506,993 + +1.5 506,993 + -0.8 506,993 + -0.2 506,993 + -1.2 506,993 + -1.4 506,993 + -2.3 506,994 - -1.5 506,994 - +0.4 506,994 - -0.2 506,994 - +0.4 506,994 - -2.7 506,994 - -0.6 506,994 - +0.3
Or see this region's nucleotide sequence