Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_00710 and AO353_00715 are separated by 211 nucleotides AO353_00715 and AO353_00720 are separated by 256 nucleotides
AO353_00710: AO353_00710 - aromatic ring-opening dioxygenase LigA, at 150,769 to 153,126
_00710
AO353_00715: AO353_00715 - cell division protein ZipA, at 153,338 to 154,186
_00715
AO353_00720: AO353_00720 - chromosome segregation protein SMC, at 154,443 to 157,931
_00720
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 153.140 kb on + strand at 153.180 kb on - strand at 153.190 kb on - strand at 153.310 kb on + strand at 153.310 kb on + strand at 153.311 kb on - strand at 154.222 kb on - strand at 154.250 kb on - strand at 154.314 kb on - strand at 154.314 kb on - strand at 154.334 kb on - strand at 154.340 kb on - strand at 154.340 kb on - strand at 154.340 kb on - strand at 154.370 kb on - strand at 154.370 kb on - strand at 154.422 kb on - strand at 154.422 kb on - strand at 154.422 kb on - strand at 154.431 kb on - strand at 154.569 kb on - strand at 154.569 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.571 kb on - strand at 154.743 kb on + strand at 154.743 kb on + strand at 154.744 kb on - strand at 154.806 kb on + strand, within AO353_00720 at 154.806 kb on + strand, within AO353_00720 at 154.807 kb on - strand, within AO353_00720 at 154.831 kb on + strand, within AO353_00720 at 154.831 kb on + strand, within AO353_00720 at 154.832 kb on - strand, within AO353_00720 at 154.832 kb on - strand, within AO353_00720 at 154.832 kb on - strand, within AO353_00720 at 154.832 kb on - strand, within AO353_00720 at 154.898 kb on + strand, within AO353_00720 at 154.898 kb on + strand, within AO353_00720 at 154.898 kb on + strand, within AO353_00720 at 154.899 kb on - strand, within AO353_00720 at 154.899 kb on - strand, within AO353_00720 at 155.023 kb on + strand, within AO353_00720 at 155.024 kb on - strand, within AO353_00720
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 153,140 + -2.0 153,180 - +1.0 153,190 - -2.5 153,310 + -1.2 153,310 + -1.8 153,311 - +0.0 154,222 - -2.9 154,250 - -1.5 154,314 - -1.4 154,314 - -1.4 154,334 - +0.6 154,340 - -1.3 154,340 - +0.2 154,340 - -1.8 154,370 - +1.6 154,370 - -1.5 154,422 - -1.4 154,422 - +1.3 154,422 - +1.2 154,431 - -1.0 154,569 - -1.8 154,569 - -0.0 154,571 - -0.3 154,571 - -1.5 154,571 - -1.4 154,571 - -0.0 154,571 - -2.1 154,571 - -1.2 154,571 - -2.2 154,571 - -1.4 154,571 - -0.0 154,571 - -0.7 154,743 + -1.0 154,743 + -1.2 154,744 - +0.5 154,806 + AO353_00720 0.10 -2.0 154,806 + AO353_00720 0.10 -0.9 154,807 - AO353_00720 0.10 -1.2 154,831 + AO353_00720 0.11 +0.5 154,831 + AO353_00720 0.11 +1.2 154,832 - AO353_00720 0.11 -0.8 154,832 - AO353_00720 0.11 +0.8 154,832 - AO353_00720 0.11 -1.3 154,832 - AO353_00720 0.11 +3.1 154,898 + AO353_00720 0.13 -0.0 154,898 + AO353_00720 0.13 -3.4 154,898 + AO353_00720 0.13 +0.2 154,899 - AO353_00720 0.13 -1.0 154,899 - AO353_00720 0.13 +1.0 155,023 + AO353_00720 0.17 +3.2 155,024 - AO353_00720 0.17 -0.5
Or see this region's nucleotide sequence