Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_00235 and AO353_00240 are separated by 42 nucleotides AO353_00240 and AO353_00245 are separated by 141 nucleotides AO353_00245 and AO353_00250 are separated by 92 nucleotides AO353_00250 and AO353_00255 are separated by 29 nucleotides AO353_00255 and AO353_00260 overlap by 4 nucleotides AO353_00260 and AO353_00265 are separated by 72 nucleotides
AO353_00235: AO353_00235 - transcriptional regulator, at 44,569 to 44,826
_00235
AO353_00240: AO353_00240 - hypothetical protein, at 44,869 to 45,048
_00240
AO353_00245: AO353_00245 - XRE family transcriptional regulator, at 45,190 to 45,537
_00245
AO353_00250: AO353_00250 - hypothetical protein, at 45,630 to 45,830
_00250
AO353_00255: AO353_00255 - hypothetical protein, at 45,860 to 46,339
_00255
AO353_00260: AO353_00260 - hypothetical protein, at 46,336 to 46,701
_00260
AO353_00265: AO353_00265 - hypothetical protein, at 46,774 to 47,007
_00265
Position (kb) 45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 44.647 kb on + strand, within AO353_00235 at 44.647 kb on + strand, within AO353_00235 at 44.647 kb on + strand, within AO353_00235 at 44.647 kb on + strand, within AO353_00235 at 44.648 kb on - strand, within AO353_00235 at 44.648 kb on - strand, within AO353_00235 at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.092 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.095 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.148 kb on - strand at 45.192 kb on - strand at 45.604 kb on - strand at 45.604 kb on - strand at 45.604 kb on - strand at 45.630 kb on - strand at 45.630 kb on - strand at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.684 kb on - strand, within AO353_00250 at 45.854 kb on + strand at 45.854 kb on + strand at 45.854 kb on + strand at 45.854 kb on + strand at 45.854 kb on + strand at 45.854 kb on + strand at 45.854 kb on + strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.855 kb on - strand at 45.876 kb on + strand at 45.876 kb on + strand at 45.987 kb on - strand at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.071 kb on - strand, within AO353_00255 at 46.075 kb on - strand, within AO353_00255 at 46.075 kb on - strand, within AO353_00255 at 46.086 kb on - strand, within AO353_00255 at 46.086 kb on - strand, within AO353_00255 at 46.102 kb on - strand, within AO353_00255 at 46.355 kb on + strand at 46.363 kb on + strand at 46.364 kb on - strand at 46.364 kb on - strand at 46.439 kb on + strand, within AO353_00260 at 46.439 kb on + strand, within AO353_00260 at 46.440 kb on - strand, within AO353_00260 at 46.477 kb on + strand, within AO353_00260 at 46.603 kb on - strand, within AO353_00260 at 46.603 kb on - strand, within AO353_00260 at 46.646 kb on - strand, within AO353_00260 at 46.646 kb on - strand, within AO353_00260 at 46.700 kb on - strand at 46.714 kb on - strand at 46.714 kb on - strand at 46.714 kb on - strand at 46.714 kb on - strand at 46.714 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 44,647 + AO353_00235 0.30 -0.9 44,647 + AO353_00235 0.30 -1.0 44,647 + AO353_00235 0.30 -2.0 44,647 + AO353_00235 0.30 +2.0 44,648 - AO353_00235 0.31 -1.3 44,648 - AO353_00235 0.31 +4.0 45,092 - -1.0 45,092 - -1.6 45,092 - -1.5 45,092 - +0.1 45,092 - -0.9 45,092 - -0.3 45,092 - -1.2 45,092 - -0.0 45,092 - +1.5 45,092 - -0.6 45,092 - +1.9 45,092 - +0.7 45,095 - -1.6 45,095 - -1.3 45,095 - +2.8 45,095 - +1.2 45,095 - -2.0 45,095 - -1.8 45,095 - +0.8 45,148 - -1.5 45,148 - -2.1 45,148 - +0.4 45,148 - -0.3 45,148 - +1.4 45,148 - -0.9 45,148 - +0.5 45,192 - -0.9 45,604 - -1.4 45,604 - -3.2 45,604 - -0.0 45,630 - -1.3 45,630 - +1.3 45,684 - AO353_00250 0.27 +1.3 45,684 - AO353_00250 0.27 +2.1 45,684 - AO353_00250 0.27 -1.2 45,684 - AO353_00250 0.27 -2.3 45,684 - AO353_00250 0.27 -0.3 45,684 - AO353_00250 0.27 +1.7 45,684 - AO353_00250 0.27 -1.6 45,684 - AO353_00250 0.27 -1.5 45,684 - AO353_00250 0.27 -1.3 45,854 + -1.0 45,854 + -1.4 45,854 + +1.8 45,854 + +1.8 45,854 + +0.8 45,854 + +1.2 45,854 + +0.3 45,855 - -0.9 45,855 - -0.8 45,855 - -0.3 45,855 - -1.2 45,855 - +0.3 45,855 - +0.1 45,855 - +1.5 45,855 - -0.5 45,855 - -0.5 45,855 - -0.0 45,876 + -0.1 45,876 + -0.0 45,987 - -0.0 46,071 - AO353_00255 0.44 +0.1 46,071 - AO353_00255 0.44 -1.2 46,071 - AO353_00255 0.44 +1.7 46,071 - AO353_00255 0.44 +1.0 46,071 - AO353_00255 0.44 -0.2 46,071 - AO353_00255 0.44 -1.0 46,071 - AO353_00255 0.44 +1.3 46,075 - AO353_00255 0.45 -2.3 46,075 - AO353_00255 0.45 +2.8 46,086 - AO353_00255 0.47 -0.6 46,086 - AO353_00255 0.47 -0.6 46,102 - AO353_00255 0.50 -0.6 46,355 + -0.2 46,363 + -0.1 46,364 - +1.7 46,364 - +0.2 46,439 + AO353_00260 0.28 -0.4 46,439 + AO353_00260 0.28 -0.5 46,440 - AO353_00260 0.28 -2.0 46,477 + AO353_00260 0.39 +0.7 46,603 - AO353_00260 0.73 +1.3 46,603 - AO353_00260 0.73 -0.3 46,646 - AO353_00260 0.85 -0.3 46,646 - AO353_00260 0.85 +0.0 46,700 - -2.1 46,714 - -0.1 46,714 - -1.6 46,714 - +1.2 46,714 - -1.0 46,714 - -0.6
Or see this region's nucleotide sequence