Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19775 and IAI47_19780 are separated by 177 nucleotides IAI47_19780 and IAI47_19785 are separated by 114 nucleotides IAI47_19785 and IAI47_19790 overlap by 20 nucleotides
IAI47_19775: IAI47_19775 - MFS transporter, at 180,058 to 181,266
_19775
IAI47_19780: IAI47_19780 - TetR/AcrR family transcriptional regulator, at 181,444 to 182,061
_19780
IAI47_19785: IAI47_19785 - DUF1232 domain-containing protein, at 182,176 to 182,562
_19785
IAI47_19790: IAI47_19790 - sigma-54-dependent Fis family transcriptional regulator, at 182,543 to 184,504
_19790
Position (kb) 182
183 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 181.282 kb on - strand at 181.289 kb on + strand at 181.289 kb on + strand at 181.290 kb on - strand at 181.296 kb on + strand at 181.297 kb on - strand at 181.300 kb on + strand at 181.300 kb on + strand at 181.300 kb on + strand at 181.301 kb on - strand at 181.320 kb on + strand at 181.321 kb on - strand at 181.321 kb on - strand at 181.336 kb on - strand at 181.351 kb on + strand at 181.367 kb on + strand at 181.368 kb on - strand at 181.369 kb on + strand at 181.370 kb on - strand at 181.395 kb on + strand at 181.398 kb on - strand at 181.444 kb on - strand at 181.572 kb on + strand, within IAI47_19780 at 181.573 kb on - strand, within IAI47_19780 at 181.591 kb on + strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.609 kb on - strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.642 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.644 kb on + strand, within IAI47_19780 at 181.645 kb on - strand, within IAI47_19780 at 181.645 kb on - strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.654 kb on + strand, within IAI47_19780 at 181.788 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.791 kb on + strand, within IAI47_19780 at 181.845 kb on + strand, within IAI47_19780 at 181.845 kb on + strand, within IAI47_19780 at 181.846 kb on - strand, within IAI47_19780 at 181.992 kb on + strand, within IAI47_19780 at 182.041 kb on + strand at 182.041 kb on + strand at 182.041 kb on + strand at 182.041 kb on + strand at 182.042 kb on - strand at 182.049 kb on + strand at 182.049 kb on + strand at 182.050 kb on - strand at 182.050 kb on - strand at 182.115 kb on + strand at 182.115 kb on + strand at 182.115 kb on + strand at 182.116 kb on - strand at 182.116 kb on - strand at 182.128 kb on + strand at 182.128 kb on + strand at 182.144 kb on + strand at 182.144 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.175 kb on + strand at 182.176 kb on - strand at 182.176 kb on - strand at 182.229 kb on - strand, within IAI47_19785 at 182.253 kb on + strand, within IAI47_19785 at 182.253 kb on + strand, within IAI47_19785 at 182.254 kb on - strand, within IAI47_19785 at 182.297 kb on - strand, within IAI47_19785 at 182.347 kb on + strand, within IAI47_19785 at 182.369 kb on + strand, within IAI47_19785 at 182.370 kb on - strand, within IAI47_19785 at 182.400 kb on + strand, within IAI47_19785 at 182.400 kb on + strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.401 kb on - strand, within IAI47_19785 at 182.451 kb on + strand, within IAI47_19785 at 182.452 kb on - strand, within IAI47_19785 at 182.452 kb on - strand, within IAI47_19785 at 182.571 kb on + strand at 182.571 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.601 kb on + strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.602 kb on - strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.603 kb on + strand at 182.604 kb on - strand at 182.604 kb on - strand at 182.604 kb on - strand at 182.676 kb on - strand at 182.676 kb on - strand at 182.680 kb on + strand at 182.680 kb on + strand at 182.681 kb on - strand at 182.681 kb on - strand at 182.834 kb on + strand, within IAI47_19790 at 182.899 kb on + strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.900 kb on - strand, within IAI47_19790 at 182.936 kb on - strand, within IAI47_19790 at 182.952 kb on - strand, within IAI47_19790 at 182.997 kb on - strand, within IAI47_19790 at 183.009 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.012 kb on + strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790 at 183.013 kb on - strand, within IAI47_19790 at 183.147 kb on - strand, within IAI47_19790 at 183.147 kb on - strand, within IAI47_19790 at 183.322 kb on + strand, within IAI47_19790 at 183.322 kb on + strand, within IAI47_19790 at 183.322 kb on + strand, within IAI47_19790 at 183.323 kb on - strand, within IAI47_19790 at 183.361 kb on + strand, within IAI47_19790 at 183.362 kb on - strand, within IAI47_19790 at 183.362 kb on - strand, within IAI47_19790 at 183.452 kb on - strand, within IAI47_19790 at 183.452 kb on - strand, within IAI47_19790 at 183.504 kb on + strand, within IAI47_19790 at 183.504 kb on + strand, within IAI47_19790 at 183.535 kb on + strand, within IAI47_19790 at 183.535 kb on + strand, within IAI47_19790 at 183.536 kb on - strand, within IAI47_19790 at 183.536 kb on - strand, within IAI47_19790
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 181,282 - -0.7 181,289 + +1.6 181,289 + -1.5 181,290 - -0.2 181,296 + -0.1 181,297 - -1.0 181,300 + -0.4 181,300 + -1.4 181,300 + +0.0 181,301 - -0.4 181,320 + -1.3 181,321 - +1.1 181,321 - -0.1 181,336 - -0.2 181,351 + +0.1 181,367 + +0.1 181,368 - -0.7 181,369 + +1.1 181,370 - +1.7 181,395 + +1.6 181,398 - -0.5 181,444 - -0.4 181,572 + IAI47_19780 0.21 +0.5 181,573 - IAI47_19780 0.21 +0.3 181,591 + IAI47_19780 0.24 +3.2 181,609 - IAI47_19780 0.27 +2.7 181,609 - IAI47_19780 0.27 -1.1 181,609 - IAI47_19780 0.27 -0.4 181,642 + IAI47_19780 0.32 +0.0 181,642 + IAI47_19780 0.32 -1.3 181,642 + IAI47_19780 0.32 +2.1 181,642 + IAI47_19780 0.32 -1.7 181,642 + IAI47_19780 0.32 +2.9 181,642 + IAI47_19780 0.32 -0.9 181,642 + IAI47_19780 0.32 -2.5 181,642 + IAI47_19780 0.32 -0.2 181,644 + IAI47_19780 0.32 -1.2 181,644 + IAI47_19780 0.32 -1.4 181,644 + IAI47_19780 0.32 +0.2 181,644 + IAI47_19780 0.32 +3.4 181,645 - IAI47_19780 0.33 +1.5 181,645 - IAI47_19780 0.33 -0.7 181,654 + IAI47_19780 0.34 -0.7 181,654 + IAI47_19780 0.34 +2.1 181,654 + IAI47_19780 0.34 +2.1 181,788 + IAI47_19780 0.56 +0.8 181,791 + IAI47_19780 0.56 +2.6 181,791 + IAI47_19780 0.56 -0.9 181,791 + IAI47_19780 0.56 -0.7 181,845 + IAI47_19780 0.65 +0.1 181,845 + IAI47_19780 0.65 +0.5 181,846 - IAI47_19780 0.65 -1.2 181,992 + IAI47_19780 0.89 +1.7 182,041 + -0.4 182,041 + -1.1 182,041 + -0.6 182,041 + -0.6 182,042 - +0.5 182,049 + -0.5 182,049 + -1.1 182,050 - -1.4 182,050 - +2.1 182,115 + -0.3 182,115 + +0.1 182,115 + -0.2 182,116 - -0.3 182,116 - +0.4 182,128 + -0.4 182,128 + -0.5 182,144 + +0.3 182,144 + +0.4 182,175 + -0.5 182,175 + +0.8 182,175 + -1.4 182,175 + +0.5 182,175 + -0.0 182,176 - +0.1 182,176 - -0.2 182,229 - IAI47_19785 0.14 +3.3 182,253 + IAI47_19785 0.20 -0.9 182,253 + IAI47_19785 0.20 -0.5 182,254 - IAI47_19785 0.20 +0.9 182,297 - IAI47_19785 0.31 +1.2 182,347 + IAI47_19785 0.44 -1.4 182,369 + IAI47_19785 0.50 +0.3 182,370 - IAI47_19785 0.50 +1.1 182,400 + IAI47_19785 0.58 +0.7 182,400 + IAI47_19785 0.58 -0.1 182,401 - IAI47_19785 0.58 +2.4 182,401 - IAI47_19785 0.58 -1.1 182,401 - IAI47_19785 0.58 +0.3 182,401 - IAI47_19785 0.58 +1.6 182,451 + IAI47_19785 0.71 -0.2 182,452 - IAI47_19785 0.71 -0.7 182,452 - IAI47_19785 0.71 -1.1 182,571 + +0.7 182,571 + +1.1 182,599 + +0.4 182,599 + +2.1 182,599 + +0.6 182,599 + -0.3 182,599 + +1.8 182,600 - -2.0 182,600 - -1.7 182,600 - +1.8 182,600 - -0.7 182,600 - -1.1 182,600 - -1.5 182,600 - +0.2 182,601 + +0.0 182,601 + +1.8 182,601 + -0.2 182,601 + +0.5 182,601 + -0.5 182,601 + -0.2 182,601 + -1.1 182,601 + -1.1 182,602 - +0.4 182,602 - +0.1 182,602 - -0.7 182,602 - +0.8 182,602 - -0.1 182,603 + +0.0 182,603 + -2.4 182,603 + -0.2 182,603 + +0.9 182,603 + -0.9 182,603 + +2.8 182,604 - +1.8 182,604 - +0.4 182,604 - +1.8 182,676 - +0.4 182,676 - +0.7 182,680 + -1.9 182,680 + -0.0 182,681 - -0.2 182,681 - +0.2 182,834 + IAI47_19790 0.15 -1.5 182,899 + IAI47_19790 0.18 -2.2 182,900 - IAI47_19790 0.18 -0.7 182,900 - IAI47_19790 0.18 +0.5 182,900 - IAI47_19790 0.18 -1.4 182,936 - IAI47_19790 0.20 +1.2 182,952 - IAI47_19790 0.21 +0.4 182,997 - IAI47_19790 0.23 -0.9 183,009 + IAI47_19790 0.24 -0.5 183,012 + IAI47_19790 0.24 +1.1 183,012 + IAI47_19790 0.24 +2.1 183,012 + IAI47_19790 0.24 -0.2 183,012 + IAI47_19790 0.24 -1.1 183,013 - IAI47_19790 0.24 -1.9 183,013 - IAI47_19790 0.24 -1.8 183,013 - IAI47_19790 0.24 +0.1 183,147 - IAI47_19790 0.31 -0.5 183,147 - IAI47_19790 0.31 -0.1 183,322 + IAI47_19790 0.40 +2.5 183,322 + IAI47_19790 0.40 -1.1 183,322 + IAI47_19790 0.40 -1.5 183,323 - IAI47_19790 0.40 +0.2 183,361 + IAI47_19790 0.42 +1.6 183,362 - IAI47_19790 0.42 -1.2 183,362 - IAI47_19790 0.42 -0.6 183,452 - IAI47_19790 0.46 +1.3 183,452 - IAI47_19790 0.46 +0.5 183,504 + IAI47_19790 0.49 -2.1 183,504 + IAI47_19790 0.49 -1.4 183,535 + IAI47_19790 0.51 -0.9 183,535 + IAI47_19790 0.51 +1.7 183,536 - IAI47_19790 0.51 +1.9 183,536 - IAI47_19790 0.51 -0.7
Or see this region's nucleotide sequence