Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_26235 and AO353_26240 are separated by 12 nucleotides AO353_26240 and AO353_26245 are separated by 81 nucleotides AO353_26245 and AO353_26250 overlap by 26 nucleotides
AO353_26235: AO353_26235 - hypothetical protein, at 5,767,942 to 5,768,907
_26235
AO353_26240: AO353_26240 - hypothetical protein, at 5,768,920 to 5,769,648
_26240
AO353_26245: AO353_26245 - phage head morphogenesis protein, at 5,769,730 to 5,770,815
_26245
AO353_26250: AO353_26250 - hypothetical protein, at 5,770,790 to 5,772,205
_26250
Position (kb) 5769
5770
5771 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5768.742 kb on - strand, within AO353_26235 at 5768.755 kb on - strand, within AO353_26235 at 5768.755 kb on - strand, within AO353_26235 at 5768.845 kb on + strand at 5768.845 kb on + strand at 5768.845 kb on + strand at 5768.845 kb on + strand at 5768.845 kb on + strand at 5768.846 kb on - strand at 5768.846 kb on - strand at 5768.846 kb on - strand at 5768.846 kb on - strand at 5768.846 kb on - strand at 5768.873 kb on + strand at 5768.873 kb on + strand at 5768.873 kb on + strand at 5768.873 kb on + strand at 5768.873 kb on + strand at 5768.874 kb on - strand at 5768.874 kb on - strand at 5768.874 kb on - strand at 5768.893 kb on + strand at 5768.894 kb on - strand at 5768.901 kb on + strand at 5768.901 kb on + strand at 5768.902 kb on - strand at 5768.902 kb on - strand at 5768.902 kb on - strand at 5768.921 kb on + strand at 5768.939 kb on + strand at 5768.940 kb on - strand at 5769.280 kb on + strand, within AO353_26240 at 5769.280 kb on + strand, within AO353_26240 at 5769.519 kb on + strand, within AO353_26240 at 5769.538 kb on + strand, within AO353_26240 at 5769.539 kb on - strand, within AO353_26240 at 5769.856 kb on + strand, within AO353_26245 at 5769.924 kb on - strand, within AO353_26245 at 5769.924 kb on - strand, within AO353_26245 at 5769.926 kb on + strand, within AO353_26245 at 5769.927 kb on - strand, within AO353_26245 at 5769.927 kb on - strand, within AO353_26245 at 5769.927 kb on - strand, within AO353_26245 at 5769.997 kb on + strand at 5769.997 kb on + strand, within AO353_26245 at 5769.998 kb on - strand, within AO353_26245 at 5769.998 kb on - strand, within AO353_26245 at 5769.998 kb on - strand, within AO353_26245 at 5770.550 kb on + strand at 5770.550 kb on + strand, within AO353_26245 at 5770.550 kb on + strand, within AO353_26245 at 5770.550 kb on + strand, within AO353_26245 at 5770.550 kb on + strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.551 kb on - strand, within AO353_26245 at 5770.606 kb on - strand, within AO353_26245 at 5770.661 kb on + strand, within AO353_26245 at 5770.923 kb on + strand at 5770.924 kb on - strand at 5770.924 kb on - strand at 5771.058 kb on + strand, within AO353_26250 at 5771.058 kb on + strand, within AO353_26250 at 5771.058 kb on + strand, within AO353_26250 at 5771.059 kb on - strand, within AO353_26250 at 5771.059 kb on - strand, within AO353_26250 at 5771.059 kb on - strand, within AO353_26250 at 5771.088 kb on + strand, within AO353_26250 at 5771.088 kb on + strand, within AO353_26250 at 5771.088 kb on + strand, within AO353_26250 at 5771.089 kb on - strand, within AO353_26250 at 5771.089 kb on - strand, within AO353_26250 at 5771.108 kb on + strand, within AO353_26250 at 5771.108 kb on + strand, within AO353_26250 at 5771.108 kb on + strand, within AO353_26250 at 5771.109 kb on - strand at 5771.170 kb on + strand, within AO353_26250 at 5771.170 kb on + strand, within AO353_26250 at 5771.170 kb on + strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.171 kb on - strand, within AO353_26250 at 5771.194 kb on + strand, within AO353_26250 at 5771.195 kb on - strand, within AO353_26250 at 5771.245 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.325 kb on + strand, within AO353_26250 at 5771.326 kb on - strand, within AO353_26250 at 5771.326 kb on - strand, within AO353_26250 at 5771.326 kb on - strand, within AO353_26250 at 5771.418 kb on + strand, within AO353_26250 at 5771.418 kb on + strand, within AO353_26250 at 5771.419 kb on - strand, within AO353_26250 at 5771.467 kb on - strand, within AO353_26250 at 5771.545 kb on + strand, within AO353_26250 at 5771.574 kb on - strand, within AO353_26250 at 5771.784 kb on + strand, within AO353_26250 at 5771.785 kb on - strand, within AO353_26250
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,768,742 - AO353_26235 0.83 +1.2 5,768,755 - AO353_26235 0.84 +0.0 5,768,755 - AO353_26235 0.84 +0.2 5,768,845 + +1.2 5,768,845 + -2.3 5,768,845 + +0.9 5,768,845 + -0.3 5,768,845 + -0.5 5,768,846 - +0.4 5,768,846 - -0.5 5,768,846 - +3.1 5,768,846 - -2.0 5,768,846 - -0.3 5,768,873 + -1.4 5,768,873 + +0.1 5,768,873 + -0.3 5,768,873 + -0.5 5,768,873 + -1.1 5,768,874 - +0.1 5,768,874 - -0.3 5,768,874 - +2.5 5,768,893 + -1.0 5,768,894 - -1.1 5,768,901 + +1.6 5,768,901 + -0.6 5,768,902 - +0.0 5,768,902 - -1.7 5,768,902 - -1.1 5,768,921 + +1.7 5,768,939 + +1.2 5,768,940 - +0.8 5,769,280 + AO353_26240 0.49 +0.6 5,769,280 + AO353_26240 0.49 -1.9 5,769,519 + AO353_26240 0.82 -0.6 5,769,538 + AO353_26240 0.85 +0.9 5,769,539 - AO353_26240 0.85 -1.1 5,769,856 + AO353_26245 0.12 -0.8 5,769,924 - AO353_26245 0.18 -0.4 5,769,924 - AO353_26245 0.18 +3.1 5,769,926 + AO353_26245 0.18 +1.6 5,769,927 - AO353_26245 0.18 +0.7 5,769,927 - AO353_26245 0.18 -0.0 5,769,927 - AO353_26245 0.18 +1.9 5,769,997 + +0.6 5,769,997 + AO353_26245 0.25 +2.3 5,769,998 - AO353_26245 0.25 -1.5 5,769,998 - AO353_26245 0.25 -2.2 5,769,998 - AO353_26245 0.25 -0.4 5,770,550 + -0.1 5,770,550 + AO353_26245 0.76 -3.1 5,770,550 + AO353_26245 0.76 -0.4 5,770,550 + AO353_26245 0.76 -1.0 5,770,550 + AO353_26245 0.76 -0.2 5,770,551 - AO353_26245 0.76 +2.3 5,770,551 - AO353_26245 0.76 -1.1 5,770,551 - AO353_26245 0.76 +0.6 5,770,551 - AO353_26245 0.76 -0.1 5,770,551 - AO353_26245 0.76 +1.5 5,770,551 - AO353_26245 0.76 -0.6 5,770,606 - AO353_26245 0.81 +2.0 5,770,661 + AO353_26245 0.86 +0.3 5,770,923 + +0.6 5,770,924 - +1.2 5,770,924 - +0.7 5,771,058 + AO353_26250 0.19 -1.6 5,771,058 + AO353_26250 0.19 -1.0 5,771,058 + AO353_26250 0.19 +1.1 5,771,059 - AO353_26250 0.19 -1.5 5,771,059 - AO353_26250 0.19 +1.2 5,771,059 - AO353_26250 0.19 -2.0 5,771,088 + AO353_26250 0.21 -1.2 5,771,088 + AO353_26250 0.21 -0.7 5,771,088 + AO353_26250 0.21 +0.3 5,771,089 - AO353_26250 0.21 -0.9 5,771,089 - AO353_26250 0.21 -0.5 5,771,108 + AO353_26250 0.22 +0.4 5,771,108 + AO353_26250 0.22 -0.9 5,771,108 + AO353_26250 0.22 -0.6 5,771,109 - +2.2 5,771,170 + AO353_26250 0.27 +0.9 5,771,170 + AO353_26250 0.27 -0.1 5,771,170 + AO353_26250 0.27 -0.9 5,771,171 - AO353_26250 0.27 +0.5 5,771,171 - AO353_26250 0.27 -1.9 5,771,171 - AO353_26250 0.27 -0.8 5,771,171 - AO353_26250 0.27 -1.1 5,771,171 - AO353_26250 0.27 -0.2 5,771,171 - AO353_26250 0.27 -2.1 5,771,171 - AO353_26250 0.27 +1.0 5,771,194 + AO353_26250 0.29 -0.4 5,771,195 - AO353_26250 0.29 +0.9 5,771,245 + AO353_26250 0.32 +0.0 5,771,325 + AO353_26250 0.38 +1.2 5,771,325 + AO353_26250 0.38 +1.7 5,771,325 + AO353_26250 0.38 -0.1 5,771,325 + AO353_26250 0.38 +0.6 5,771,325 + AO353_26250 0.38 -2.8 5,771,325 + AO353_26250 0.38 -0.7 5,771,325 + AO353_26250 0.38 +1.9 5,771,326 - AO353_26250 0.38 -0.3 5,771,326 - AO353_26250 0.38 -2.8 5,771,326 - AO353_26250 0.38 -1.1 5,771,418 + AO353_26250 0.44 -0.7 5,771,418 + AO353_26250 0.44 -0.7 5,771,419 - AO353_26250 0.44 -0.5 5,771,467 - AO353_26250 0.48 +0.6 5,771,545 + AO353_26250 0.53 -1.1 5,771,574 - AO353_26250 0.55 -2.8 5,771,784 + AO353_26250 0.70 -1.7 5,771,785 - AO353_26250 0.70 +1.0
Or see this region's nucleotide sequence