Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25300 and AO353_25305 are separated by 137 nucleotides AO353_25305 and AO353_25310 are separated by 1 nucleotides AO353_25310 and AO353_25315 are separated by 64 nucleotides AO353_25315 and AO353_25320 overlap by 4 nucleotides
AO353_25300: AO353_25300 - LysR family transcriptional regulator, at 5,582,205 to 5,583,119
_25300
AO353_25305: AO353_25305 - malonate transporter, at 5,583,257 to 5,584,021
_25305
AO353_25310: AO353_25310 - malonate carrier protein, at 5,584,023 to 5,584,439
_25310
AO353_25315: AO353_25315 - malonate decarboxylase subunit epsilon, at 5,584,504 to 5,585,424
_25315
AO353_25320: AO353_25320 - phosphoribosyl-dephospho-CoA transferase, at 5,585,421 to 5,586,041
_25320
Position (kb) 5584
5585 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5583.099 kb on + strand at 5583.099 kb on + strand at 5583.099 kb on + strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.100 kb on - strand at 5583.165 kb on + strand at 5583.165 kb on + strand at 5583.165 kb on + strand at 5583.165 kb on + strand at 5583.166 kb on - strand at 5583.166 kb on - strand at 5583.166 kb on - strand at 5583.190 kb on + strand at 5583.259 kb on - strand at 5583.285 kb on + strand at 5583.579 kb on + strand, within AO353_25305 at 5583.579 kb on + strand, within AO353_25305 at 5583.579 kb on + strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.580 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5583.662 kb on - strand, within AO353_25305 at 5584.021 kb on + strand at 5584.021 kb on + strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.022 kb on - strand at 5584.228 kb on + strand, within AO353_25310 at 5584.228 kb on + strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.229 kb on - strand, within AO353_25310 at 5584.429 kb on + strand at 5584.429 kb on + strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.434 kb on - strand at 5584.471 kb on + strand at 5584.471 kb on + strand at 5584.472 kb on - strand at 5584.675 kb on + strand, within AO353_25315 at 5584.826 kb on - strand, within AO353_25315 at 5585.199 kb on - strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.208 kb on + strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.209 kb on - strand, within AO353_25315 at 5585.308 kb on + strand, within AO353_25315
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,583,099 + +1.0 5,583,099 + -1.0 5,583,099 + +0.0 5,583,100 - +0.5 5,583,100 - -0.7 5,583,100 - -0.3 5,583,100 - +0.0 5,583,100 - -0.6 5,583,100 - -1.3 5,583,100 - -0.1 5,583,165 + +0.8 5,583,165 + +1.3 5,583,165 + -0.4 5,583,165 + +1.0 5,583,166 - +0.1 5,583,166 - -0.2 5,583,166 - -1.1 5,583,190 + -0.0 5,583,259 - +0.9 5,583,285 + -0.8 5,583,579 + AO353_25305 0.42 -1.1 5,583,579 + AO353_25305 0.42 -0.3 5,583,579 + AO353_25305 0.42 +0.2 5,583,580 - AO353_25305 0.42 -1.6 5,583,580 - AO353_25305 0.42 -0.1 5,583,580 - AO353_25305 0.42 +1.1 5,583,580 - AO353_25305 0.42 +0.2 5,583,580 - AO353_25305 0.42 -1.7 5,583,580 - AO353_25305 0.42 -1.5 5,583,662 - AO353_25305 0.53 -1.8 5,583,662 - AO353_25305 0.53 +1.6 5,583,662 - AO353_25305 0.53 +0.9 5,584,021 + -1.3 5,584,021 + +0.6 5,584,022 - +1.4 5,584,022 - +1.2 5,584,022 - -0.7 5,584,022 - +0.6 5,584,228 + AO353_25310 0.49 -1.5 5,584,228 + AO353_25310 0.49 -0.9 5,584,229 - AO353_25310 0.49 -1.7 5,584,229 - AO353_25310 0.49 +0.0 5,584,229 - AO353_25310 0.49 -0.1 5,584,229 - AO353_25310 0.49 +1.0 5,584,229 - AO353_25310 0.49 -0.4 5,584,429 + +1.0 5,584,429 + +0.1 5,584,434 - -1.6 5,584,434 - -1.1 5,584,434 - +1.7 5,584,434 - -1.3 5,584,471 + +0.4 5,584,471 + -2.2 5,584,472 - +0.7 5,584,675 + AO353_25315 0.19 -0.7 5,584,826 - AO353_25315 0.35 +1.3 5,585,199 - AO353_25315 0.75 +2.9 5,585,208 + AO353_25315 0.76 +2.3 5,585,208 + AO353_25315 0.76 -0.4 5,585,208 + AO353_25315 0.76 -0.9 5,585,208 + AO353_25315 0.76 +2.5 5,585,208 + AO353_25315 0.76 +1.0 5,585,209 - AO353_25315 0.77 -1.9 5,585,209 - AO353_25315 0.77 +1.7 5,585,209 - AO353_25315 0.77 -0.7 5,585,209 - AO353_25315 0.77 -0.9 5,585,209 - AO353_25315 0.77 -1.4 5,585,308 + AO353_25315 0.87 -0.1
Or see this region's nucleotide sequence