Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_24755 and AO353_24760 are separated by 12 nucleotides AO353_24760 and AO353_24765 are separated by 108 nucleotides AO353_24765 and AO353_24770 are separated by 36 nucleotides
AO353_24755: AO353_24755 - ATP-dependent DNA helicase, at 5,455,964 to 5,458,228
_24755
AO353_24760: AO353_24760 - glutamine amidotransferase, at 5,458,241 to 5,459,038
_24760
AO353_24765: AO353_24765 - GCN5 family acetyltransferase, at 5,459,147 to 5,459,644
_24765
AO353_24770: AO353_24770 - FAD-dependent oxidoreductase, at 5,459,681 to 5,460,991
_24770
Position (kb)
5458
5459
5460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5457.246 kb on - strand, within AO353_24755 at 5457.308 kb on + strand, within AO353_24755 at 5457.309 kb on - strand, within AO353_24755 at 5457.309 kb on - strand, within AO353_24755 at 5457.356 kb on + strand, within AO353_24755 at 5457.356 kb on + strand, within AO353_24755 at 5457.356 kb on + strand, within AO353_24755 at 5457.357 kb on - strand, within AO353_24755 at 5457.357 kb on - strand, within AO353_24755 at 5457.357 kb on - strand, within AO353_24755 at 5457.560 kb on + strand, within AO353_24755 at 5457.563 kb on + strand, within AO353_24755 at 5457.564 kb on - strand at 5457.747 kb on - strand, within AO353_24755 at 5457.774 kb on - strand, within AO353_24755 at 5457.941 kb on - strand, within AO353_24755 at 5458.058 kb on + strand at 5458.059 kb on - strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.064 kb on + strand at 5458.065 kb on - strand at 5458.065 kb on - strand at 5458.065 kb on - strand at 5458.065 kb on - strand at 5458.065 kb on - strand at 5458.065 kb on - strand at 5458.070 kb on + strand at 5458.070 kb on + strand at 5458.071 kb on - strand at 5458.071 kb on - strand at 5458.071 kb on - strand at 5458.071 kb on - strand at 5458.382 kb on - strand, within AO353_24760 at 5458.494 kb on + strand, within AO353_24760 at 5458.494 kb on + strand, within AO353_24760 at 5458.495 kb on - strand, within AO353_24760 at 5458.504 kb on - strand at 5458.767 kb on + strand, within AO353_24760 at 5458.781 kb on + strand, within AO353_24760 at 5458.791 kb on + strand, within AO353_24760 at 5458.917 kb on + strand, within AO353_24760 at 5459.008 kb on - strand at 5459.008 kb on - strand at 5459.075 kb on + strand at 5459.075 kb on + strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.382 kb on + strand, within AO353_24765 at 5459.394 kb on + strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.496 kb on + strand, within AO353_24765 at 5459.568 kb on + strand, within AO353_24765 at 5459.569 kb on - strand, within AO353_24765 at 5459.574 kb on + strand, within AO353_24765 at 5459.574 kb on + strand, within AO353_24765 at 5459.575 kb on - strand at 5459.575 kb on - strand, within AO353_24765 at 5459.575 kb on - strand, within AO353_24765 at 5459.575 kb on - strand, within AO353_24765 at 5459.687 kb on + strand at 5459.688 kb on - strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.738 kb on - strand at 5459.738 kb on - strand at 5459.738 kb on - strand at 5459.874 kb on + strand, within AO353_24770 at 5459.875 kb on - strand, within AO353_24770 at 5459.886 kb on + strand at 5459.886 kb on + strand, within AO353_24770 at 5459.886 kb on + strand, within AO353_24770 at 5459.886 kb on + strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.967 kb on + strand, within AO353_24770 at 5459.968 kb on - strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.025 kb on - strand, within AO353_24770 at 5460.025 kb on - strand, within AO353_24770
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,457,246 - AO353_24755 0.57 -1.3 5,457,308 + AO353_24755 0.59 -2.0 5,457,309 - AO353_24755 0.59 +2.5 5,457,309 - AO353_24755 0.59 +0.6 5,457,356 + AO353_24755 0.61 +0.1 5,457,356 + AO353_24755 0.61 -0.7 5,457,356 + AO353_24755 0.61 -1.7 5,457,357 - AO353_24755 0.62 -1.3 5,457,357 - AO353_24755 0.62 +1.7 5,457,357 - AO353_24755 0.62 +0.5 5,457,560 + AO353_24755 0.70 +1.3 5,457,563 + AO353_24755 0.71 +2.0 5,457,564 - +0.5 5,457,747 - AO353_24755 0.79 +1.3 5,457,774 - AO353_24755 0.80 +0.1 5,457,941 - AO353_24755 0.87 +0.2 5,458,058 + +1.4 5,458,059 - +0.6 5,458,064 + -2.9 5,458,064 + -1.3 5,458,064 + -0.1 5,458,064 + -1.6 5,458,064 + -1.4 5,458,064 + -1.3 5,458,064 + -1.6 5,458,064 + -1.0 5,458,064 + -2.2 5,458,065 - -2.0 5,458,065 - -0.7 5,458,065 - +1.9 5,458,065 - -1.4 5,458,065 - -1.8 5,458,065 - +0.1 5,458,070 + -0.7 5,458,070 + -0.5 5,458,071 - -0.2 5,458,071 - -2.5 5,458,071 - +1.1 5,458,071 - +0.9 5,458,382 - AO353_24760 0.18 +1.1 5,458,494 + AO353_24760 0.32 +1.9 5,458,494 + AO353_24760 0.32 -0.6 5,458,495 - AO353_24760 0.32 -2.4 5,458,504 - +2.7 5,458,767 + AO353_24760 0.66 -1.1 5,458,781 + AO353_24760 0.68 -2.1 5,458,791 + AO353_24760 0.69 +1.4 5,458,917 + AO353_24760 0.85 -1.3 5,459,008 - -2.1 5,459,008 - +0.9 5,459,075 + -1.5 5,459,075 + +0.6 5,459,076 - +0.6 5,459,076 - +1.2 5,459,076 - -0.1 5,459,076 - -0.8 5,459,382 + AO353_24765 0.47 -1.7 5,459,394 + AO353_24765 0.50 +2.1 5,459,395 - AO353_24765 0.50 -0.4 5,459,395 - AO353_24765 0.50 +0.4 5,459,395 - AO353_24765 0.50 +1.9 5,459,496 + AO353_24765 0.70 -0.6 5,459,568 + AO353_24765 0.85 -2.1 5,459,569 - AO353_24765 0.85 +0.6 5,459,574 + AO353_24765 0.86 +0.3 5,459,574 + AO353_24765 0.86 +0.5 5,459,575 - +0.9 5,459,575 - AO353_24765 0.86 -1.6 5,459,575 - AO353_24765 0.86 -2.5 5,459,575 - AO353_24765 0.86 -0.1 5,459,687 + -0.7 5,459,688 - -1.7 5,459,737 + -0.3 5,459,737 + -1.1 5,459,737 + -1.2 5,459,737 + -0.9 5,459,738 - -0.3 5,459,738 - -0.1 5,459,738 - -2.1 5,459,874 + AO353_24770 0.15 +1.3 5,459,875 - AO353_24770 0.15 +0.9 5,459,886 + +3.1 5,459,886 + AO353_24770 0.16 +5.0 5,459,886 + AO353_24770 0.16 -0.9 5,459,886 + AO353_24770 0.16 -0.7 5,459,887 - AO353_24770 0.16 -0.7 5,459,887 - AO353_24770 0.16 +0.5 5,459,887 - AO353_24770 0.16 -1.6 5,459,967 + AO353_24770 0.22 +0.6 5,459,968 - AO353_24770 0.22 +0.9 5,460,024 + AO353_24770 0.26 -1.7 5,460,024 + AO353_24770 0.26 +1.1 5,460,024 + AO353_24770 0.26 +0.9 5,460,025 - AO353_24770 0.26 -0.4 5,460,025 - AO353_24770 0.26 -1.2
Or see this region's nucleotide sequence