Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23015 and AO353_23020 are separated by 83 nucleotides AO353_23020 and AO353_23025 are separated by 3 nucleotides AO353_23025 and AO353_23030 are separated by 314 nucleotides
AO353_23015: AO353_23015 - histidine kinase, at 5,051,680 to 5,053,065
_23015
AO353_23020: AO353_23020 - hypothetical protein, at 5,053,149 to 5,053,520
_23020
AO353_23025: AO353_23025 - Fe-S oxidoreductase, at 5,053,524 to 5,053,697
_23025
AO353_23030: AO353_23030 - sulfate transporter, at 5,054,012 to 5,055,457
_23030
Position (kb)
5053
5054 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5052.604 kb on + strand, within AO353_23015 at 5052.651 kb on + strand, within AO353_23015 at 5052.652 kb on - strand, within AO353_23015 at 5052.753 kb on + strand, within AO353_23015 at 5052.753 kb on + strand, within AO353_23015 at 5052.754 kb on - strand, within AO353_23015 at 5052.754 kb on - strand, within AO353_23015 at 5052.774 kb on - strand, within AO353_23015 at 5052.774 kb on - strand at 5052.774 kb on - strand, within AO353_23015 at 5052.777 kb on + strand, within AO353_23015 at 5052.777 kb on + strand, within AO353_23015 at 5052.777 kb on + strand, within AO353_23015 at 5052.778 kb on - strand, within AO353_23015 at 5052.778 kb on - strand, within AO353_23015 at 5053.014 kb on - strand at 5053.063 kb on + strand at 5053.129 kb on - strand at 5053.156 kb on + strand at 5053.157 kb on - strand at 5053.157 kb on - strand at 5053.157 kb on - strand at 5053.237 kb on + strand, within AO353_23020 at 5053.237 kb on + strand, within AO353_23020 at 5053.237 kb on + strand, within AO353_23020 at 5053.237 kb on + strand, within AO353_23020 at 5053.238 kb on - strand, within AO353_23020 at 5053.238 kb on - strand, within AO353_23020 at 5053.238 kb on - strand, within AO353_23020 at 5053.272 kb on + strand, within AO353_23020 at 5053.272 kb on + strand, within AO353_23020 at 5053.273 kb on - strand, within AO353_23020 at 5053.273 kb on - strand, within AO353_23020 at 5053.273 kb on - strand, within AO353_23020 at 5053.550 kb on + strand, within AO353_23025 at 5053.551 kb on - strand, within AO353_23025 at 5053.551 kb on - strand, within AO353_23025 at 5053.709 kb on + strand at 5053.723 kb on + strand at 5053.723 kb on + strand at 5053.723 kb on + strand at 5053.732 kb on + strand at 5053.732 kb on + strand at 5053.732 kb on + strand at 5053.732 kb on + strand at 5053.732 kb on + strand at 5053.733 kb on - strand at 5053.733 kb on - strand at 5053.733 kb on - strand at 5053.804 kb on + strand at 5053.805 kb on - strand at 5053.880 kb on + strand at 5053.890 kb on + strand at 5053.891 kb on - strand at 5053.916 kb on - strand at 5054.007 kb on - strand at 5054.130 kb on - strand at 5054.253 kb on - strand, within AO353_23030 at 5054.445 kb on - strand, within AO353_23030 at 5054.445 kb on - strand, within AO353_23030 at 5054.486 kb on + strand, within AO353_23030 at 5054.487 kb on - strand, within AO353_23030 at 5054.487 kb on - strand at 5054.487 kb on - strand, within AO353_23030 at 5054.487 kb on - strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.490 kb on + strand, within AO353_23030 at 5054.491 kb on - strand, within AO353_23030 at 5054.493 kb on + strand, within AO353_23030
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,052,604 + AO353_23015 0.67 +0.7 5,052,651 + AO353_23015 0.70 -1.7 5,052,652 - AO353_23015 0.70 +0.7 5,052,753 + AO353_23015 0.77 +0.7 5,052,753 + AO353_23015 0.77 -1.0 5,052,754 - AO353_23015 0.77 +0.4 5,052,754 - AO353_23015 0.77 -1.9 5,052,774 - AO353_23015 0.79 -0.9 5,052,774 - +0.4 5,052,774 - AO353_23015 0.79 -1.7 5,052,777 + AO353_23015 0.79 -2.4 5,052,777 + AO353_23015 0.79 -0.0 5,052,777 + AO353_23015 0.79 -0.3 5,052,778 - AO353_23015 0.79 -2.0 5,052,778 - AO353_23015 0.79 -0.0 5,053,014 - -0.6 5,053,063 + -2.3 5,053,129 - +0.8 5,053,156 + +0.5 5,053,157 - -1.0 5,053,157 - -0.1 5,053,157 - +1.3 5,053,237 + AO353_23020 0.24 -0.5 5,053,237 + AO353_23020 0.24 +2.0 5,053,237 + AO353_23020 0.24 -2.2 5,053,237 + AO353_23020 0.24 -1.8 5,053,238 - AO353_23020 0.24 -0.8 5,053,238 - AO353_23020 0.24 +0.7 5,053,238 - AO353_23020 0.24 -0.8 5,053,272 + AO353_23020 0.33 -1.1 5,053,272 + AO353_23020 0.33 -0.0 5,053,273 - AO353_23020 0.33 -0.6 5,053,273 - AO353_23020 0.33 +0.9 5,053,273 - AO353_23020 0.33 -2.3 5,053,550 + AO353_23025 0.15 +3.5 5,053,551 - AO353_23025 0.16 +1.3 5,053,551 - AO353_23025 0.16 +0.7 5,053,709 + +3.4 5,053,723 + -2.7 5,053,723 + -0.3 5,053,723 + +0.3 5,053,732 + -0.1 5,053,732 + +0.7 5,053,732 + -0.8 5,053,732 + -0.5 5,053,732 + -1.6 5,053,733 - -2.5 5,053,733 - -2.1 5,053,733 - +1.0 5,053,804 + +1.0 5,053,805 - +1.0 5,053,880 + -0.0 5,053,890 + -1.2 5,053,891 - -1.0 5,053,916 - +0.2 5,054,007 - -0.0 5,054,130 - -0.3 5,054,253 - AO353_23030 0.17 +0.6 5,054,445 - AO353_23030 0.30 +0.8 5,054,445 - AO353_23030 0.30 +2.1 5,054,486 + AO353_23030 0.33 -3.7 5,054,487 - AO353_23030 0.33 +0.9 5,054,487 - -0.6 5,054,487 - AO353_23030 0.33 +0.6 5,054,487 - AO353_23030 0.33 -3.3 5,054,490 + AO353_23030 0.33 -1.0 5,054,490 + AO353_23030 0.33 +0.1 5,054,490 + AO353_23030 0.33 -0.7 5,054,490 + AO353_23030 0.33 +2.9 5,054,490 + AO353_23030 0.33 +3.4 5,054,490 + AO353_23030 0.33 +0.4 5,054,490 + AO353_23030 0.33 -1.2 5,054,490 + AO353_23030 0.33 +0.4 5,054,490 + AO353_23030 0.33 +0.3 5,054,490 + AO353_23030 0.33 +2.0 5,054,491 - AO353_23030 0.33 -0.0 5,054,493 + AO353_23030 0.33 +1.1
Or see this region's nucleotide sequence