Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_22725 and AO353_22730 overlap by 4 nucleotides AO353_22730 and AO353_22735 are separated by 208 nucleotides AO353_22735 and AO353_22740 are separated by 77 nucleotides
AO353_22725: AO353_22725 - hypothetical protein, at 4,977,308 to 4,977,679
_22725
AO353_22730: AO353_22730 - hypothetical protein, at 4,977,676 to 4,978,335
_22730
AO353_22735: AO353_22735 - hypothetical protein, at 4,978,544 to 4,978,783
_22735
AO353_22740: AO353_22740 - sterol desaturase, at 4,978,861 to 4,980,015
_22740
Position (kb)
4978
4979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4977.554 kb on + strand, within AO353_22725 at 4977.554 kb on + strand, within AO353_22725 at 4977.554 kb on + strand, within AO353_22725 at 4977.555 kb on - strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.632 kb on + strand, within AO353_22725 at 4977.761 kb on - strand, within AO353_22730 at 4977.769 kb on + strand, within AO353_22730 at 4977.770 kb on - strand, within AO353_22730 at 4977.770 kb on - strand, within AO353_22730 at 4977.770 kb on - strand, within AO353_22730 at 4977.841 kb on + strand, within AO353_22730 at 4977.841 kb on + strand, within AO353_22730 at 4977.842 kb on - strand, within AO353_22730 at 4977.842 kb on - strand, within AO353_22730 at 4977.842 kb on - strand, within AO353_22730 at 4977.861 kb on + strand, within AO353_22730 at 4977.861 kb on + strand, within AO353_22730 at 4977.861 kb on + strand, within AO353_22730 at 4977.861 kb on + strand, within AO353_22730 at 4977.866 kb on - strand, within AO353_22730 at 4977.886 kb on + strand, within AO353_22730 at 4977.889 kb on + strand, within AO353_22730 at 4977.889 kb on + strand, within AO353_22730 at 4977.889 kb on + strand, within AO353_22730 at 4977.889 kb on + strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4977.890 kb on - strand, within AO353_22730 at 4978.012 kb on + strand, within AO353_22730 at 4978.012 kb on + strand, within AO353_22730 at 4978.013 kb on - strand, within AO353_22730 at 4978.013 kb on - strand, within AO353_22730 at 4978.013 kb on - strand, within AO353_22730 at 4978.017 kb on + strand, within AO353_22730 at 4978.030 kb on + strand, within AO353_22730 at 4978.030 kb on + strand, within AO353_22730 at 4978.031 kb on - strand at 4978.042 kb on + strand, within AO353_22730 at 4978.042 kb on + strand, within AO353_22730 at 4978.042 kb on + strand, within AO353_22730 at 4978.042 kb on + strand, within AO353_22730 at 4978.043 kb on - strand, within AO353_22730 at 4978.043 kb on - strand, within AO353_22730 at 4978.043 kb on - strand, within AO353_22730 at 4978.043 kb on - strand, within AO353_22730 at 4978.043 kb on - strand, within AO353_22730 at 4978.300 kb on + strand at 4978.321 kb on + strand at 4978.322 kb on - strand at 4978.322 kb on - strand at 4978.385 kb on + strand at 4978.385 kb on + strand at 4978.386 kb on - strand at 4978.453 kb on + strand at 4978.454 kb on - strand at 4978.565 kb on + strand at 4978.566 kb on - strand at 4978.646 kb on + strand, within AO353_22735 at 4978.646 kb on + strand, within AO353_22735 at 4978.646 kb on + strand, within AO353_22735 at 4978.647 kb on - strand, within AO353_22735 at 4978.647 kb on - strand, within AO353_22735 at 4978.860 kb on - strand at 4979.054 kb on + strand, within AO353_22740 at 4979.054 kb on + strand, within AO353_22740 at 4979.055 kb on - strand, within AO353_22740 at 4979.055 kb on - strand, within AO353_22740 at 4979.120 kb on + strand, within AO353_22740 at 4979.121 kb on - strand, within AO353_22740 at 4979.121 kb on - strand, within AO353_22740 at 4979.121 kb on - strand, within AO353_22740 at 4979.121 kb on - strand at 4979.121 kb on - strand, within AO353_22740 at 4979.379 kb on - strand, within AO353_22740 at 4979.396 kb on + strand, within AO353_22740 at 4979.396 kb on + strand, within AO353_22740 at 4979.396 kb on + strand, within AO353_22740 at 4979.397 kb on - strand, within AO353_22740 at 4979.467 kb on + strand, within AO353_22740 at 4979.549 kb on + strand, within AO353_22740 at 4979.559 kb on - strand at 4979.708 kb on + strand, within AO353_22740 at 4979.708 kb on + strand, within AO353_22740 at 4979.708 kb on + strand, within AO353_22740 at 4979.762 kb on + strand, within AO353_22740
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,977,554 + AO353_22725 0.66 +2.1 4,977,554 + AO353_22725 0.66 +2.0 4,977,554 + AO353_22725 0.66 +1.3 4,977,555 - AO353_22725 0.66 +0.8 4,977,632 + AO353_22725 0.87 -0.8 4,977,632 + AO353_22725 0.87 -0.1 4,977,632 + AO353_22725 0.87 +0.6 4,977,632 + AO353_22725 0.87 -0.3 4,977,632 + AO353_22725 0.87 +2.2 4,977,632 + AO353_22725 0.87 +0.7 4,977,632 + AO353_22725 0.87 +0.4 4,977,632 + AO353_22725 0.87 +3.4 4,977,632 + AO353_22725 0.87 +0.4 4,977,761 - AO353_22730 0.13 -0.5 4,977,769 + AO353_22730 0.14 -0.0 4,977,770 - AO353_22730 0.14 +1.0 4,977,770 - AO353_22730 0.14 -2.6 4,977,770 - AO353_22730 0.14 +0.7 4,977,841 + AO353_22730 0.25 +1.6 4,977,841 + AO353_22730 0.25 +1.0 4,977,842 - AO353_22730 0.25 -0.3 4,977,842 - AO353_22730 0.25 -0.8 4,977,842 - AO353_22730 0.25 +2.2 4,977,861 + AO353_22730 0.28 +0.4 4,977,861 + AO353_22730 0.28 +0.9 4,977,861 + AO353_22730 0.28 -0.5 4,977,861 + AO353_22730 0.28 -0.0 4,977,866 - AO353_22730 0.29 -0.0 4,977,886 + AO353_22730 0.32 -1.2 4,977,889 + AO353_22730 0.32 +2.2 4,977,889 + AO353_22730 0.32 +0.3 4,977,889 + AO353_22730 0.32 -1.3 4,977,889 + AO353_22730 0.32 -0.2 4,977,890 - AO353_22730 0.32 +0.8 4,977,890 - AO353_22730 0.32 +0.8 4,977,890 - AO353_22730 0.32 -1.5 4,977,890 - AO353_22730 0.32 +1.9 4,977,890 - AO353_22730 0.32 -2.7 4,977,890 - AO353_22730 0.32 +1.1 4,977,890 - AO353_22730 0.32 -1.0 4,978,012 + AO353_22730 0.51 +0.3 4,978,012 + AO353_22730 0.51 +0.5 4,978,013 - AO353_22730 0.51 -0.8 4,978,013 - AO353_22730 0.51 -0.7 4,978,013 - AO353_22730 0.51 +0.4 4,978,017 + AO353_22730 0.52 -1.9 4,978,030 + AO353_22730 0.54 -2.2 4,978,030 + AO353_22730 0.54 +2.6 4,978,031 - +3.0 4,978,042 + AO353_22730 0.55 +2.2 4,978,042 + AO353_22730 0.55 -2.8 4,978,042 + AO353_22730 0.55 -0.0 4,978,042 + AO353_22730 0.55 +1.5 4,978,043 - AO353_22730 0.56 -1.4 4,978,043 - AO353_22730 0.56 +0.0 4,978,043 - AO353_22730 0.56 +1.1 4,978,043 - AO353_22730 0.56 -0.8 4,978,043 - AO353_22730 0.56 +0.6 4,978,300 + -0.0 4,978,321 + +2.0 4,978,322 - -0.4 4,978,322 - -1.3 4,978,385 + -0.8 4,978,385 + -2.4 4,978,386 - -0.2 4,978,453 + -2.0 4,978,454 - -1.3 4,978,565 + +0.9 4,978,566 - -0.2 4,978,646 + AO353_22735 0.42 +2.1 4,978,646 + AO353_22735 0.42 +0.6 4,978,646 + AO353_22735 0.42 +1.5 4,978,647 - AO353_22735 0.43 -0.2 4,978,647 - AO353_22735 0.43 -0.8 4,978,860 - -1.4 4,979,054 + AO353_22740 0.17 -0.4 4,979,054 + AO353_22740 0.17 +1.4 4,979,055 - AO353_22740 0.17 +0.9 4,979,055 - AO353_22740 0.17 +0.4 4,979,120 + AO353_22740 0.22 -0.7 4,979,121 - AO353_22740 0.23 +0.9 4,979,121 - AO353_22740 0.23 -0.9 4,979,121 - AO353_22740 0.23 +2.0 4,979,121 - +1.0 4,979,121 - AO353_22740 0.23 -1.2 4,979,379 - AO353_22740 0.45 +1.1 4,979,396 + AO353_22740 0.46 -0.1 4,979,396 + AO353_22740 0.46 -0.3 4,979,396 + AO353_22740 0.46 -0.3 4,979,397 - AO353_22740 0.46 -2.0 4,979,467 + AO353_22740 0.52 +0.9 4,979,549 + AO353_22740 0.60 +0.5 4,979,559 - -0.6 4,979,708 + AO353_22740 0.73 +0.3 4,979,708 + AO353_22740 0.73 +1.1 4,979,708 + AO353_22740 0.73 -2.3 4,979,762 + AO353_22740 0.78 -0.3
Or see this region's nucleotide sequence