Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_13130

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_13120 and AO353_13125 overlap by 13 nucleotidesAO353_13125 and AO353_13130 are separated by 0 nucleotidesAO353_13130 and AO353_13135 overlap by 4 nucleotides AO353_13120: AO353_13120 - carbohydrate kinase, at 2,907,222 to 2,908,721 _13120 AO353_13125: AO353_13125 - tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, at 2,908,709 to 2,909,179 _13125 AO353_13130: AO353_13130 - N-acetylmuramoyl-L-alanine amidase, at 2,909,180 to 2,910,616 _13130 AO353_13135: AO353_13135 - DNA mismatch repair protein, at 2,910,613 to 2,912,523 _13135 Position (kb) 2909 2910 2911Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2908.352 kb on - strand, within AO353_13120at 2908.419 kb on + strand, within AO353_13120at 2908.419 kb on + strand, within AO353_13120at 2908.637 kb on + strandat 2908.637 kb on + strandat 2908.638 kb on - strandat 2908.638 kb on - strandat 2908.719 kb on + strandat 2908.719 kb on + strandat 2908.719 kb on + strandat 2908.727 kb on + strandat 2909.177 kb on + strandat 2909.177 kb on + strandat 2909.194 kb on + strandat 2909.194 kb on + strandat 2909.194 kb on + strandat 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.410 kb on + strand, within AO353_13130at 2910.411 kb on - strand, within AO353_13130at 2910.759 kb on + strandat 2910.759 kb on + strandat 2910.759 kb on + strandat 2910.760 kb on - strandat 2911.397 kb on - strand, within AO353_13135at 2911.459 kb on + strand, within AO353_13135at 2911.600 kb on + strand, within AO353_13135at 2911.600 kb on + strand, within AO353_13135at 2911.600 kb on + strand, within AO353_13135at 2911.601 kb on - strand, within AO353_13135at 2911.601 kb on - strand, within AO353_13135at 2911.601 kb on - strand, within AO353_13135

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,908,352 - AO353_13120 0.75 +0.6
2,908,419 + AO353_13120 0.80 +0.8
2,908,419 + AO353_13120 0.80 +3.2
2,908,637 + +2.0
2,908,637 + +1.0
2,908,638 - +0.4
2,908,638 - -2.0
2,908,719 + -1.9
2,908,719 + -1.9
2,908,719 + -1.0
2,908,727 + -1.1
2,909,177 + +0.1
2,909,177 + -1.7
2,909,194 + +1.0
2,909,194 + -2.1
2,909,194 + -1.2
2,910,410 + AO353_13130 0.86 -2.0
2,910,410 + AO353_13130 0.86 -1.5
2,910,410 + AO353_13130 0.86 -2.7
2,910,410 + AO353_13130 0.86 -1.7
2,910,410 + AO353_13130 0.86 -0.5
2,910,410 + AO353_13130 0.86 -1.3
2,910,410 + AO353_13130 0.86 -0.5
2,910,411 - AO353_13130 0.86 +0.6
2,910,759 + -0.1
2,910,759 + +2.1
2,910,759 + -0.1
2,910,760 - +0.8
2,911,397 - AO353_13135 0.41 +1.1
2,911,459 + AO353_13135 0.44 +1.2
2,911,600 + AO353_13135 0.52 -1.8
2,911,600 + AO353_13135 0.52 -2.0
2,911,600 + AO353_13135 0.52 -1.0
2,911,601 - AO353_13135 0.52 +0.1
2,911,601 - AO353_13135 0.52 +0.1
2,911,601 - AO353_13135 0.52 -0.9

Or see this region's nucleotide sequence