Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_06050 and AO353_06055 are separated by 154 nucleotides AO353_06055 and AO353_06060 are separated by 146 nucleotides AO353_06060 and AO353_06065 are separated by 227 nucleotides AO353_06065 and AO353_06070 are separated by 34 nucleotides AO353_06070 and AO353_06075 are separated by 72 nucleotides
AO353_06050: AO353_06050 - exodeoxyribonuclease V subunit gamma, at 1,346,383 to 1,349,835
_06050
AO353_06055: AO353_06055 - 5S ribosomal RNA, at 1,349,990 to 1,350,105
_06055
AO353_06060: AO353_06060 - 23S ribosomal RNA, at 1,350,252 to 1,353,164
_06060
AO353_06065: AO353_06065 - tRNA-Ala, at 1,353,392 to 1,353,467
_06065
AO353_06070: AO353_06070 - tRNA-Ile, at 1,353,502 to 1,353,578
_06070
AO353_06075: AO353_06075 - 16S ribosomal RNA, at 1,353,651 to 1,355,197
_06075
Position (kb)
1350
1351
1352
1353
1354 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1349.415 kb on + strand, within AO353_06050 at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.864 kb on + strand at 1349.864 kb on + strand at 1349.865 kb on - strand at 1349.865 kb on - strand at 1349.877 kb on + strand at 1349.877 kb on + strand at 1349.925 kb on + strand at 1349.925 kb on + strand at 1349.925 kb on + strand at 1349.926 kb on - strand at 1349.926 kb on - strand at 1349.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,349,415 + AO353_06050 0.88 -0.2 1,349,803 - -0.7 1,349,803 - +1.3 1,349,803 - -2.0 1,349,803 - +0.9 1,349,864 + -1.2 1,349,864 + -0.2 1,349,865 - +0.7 1,349,865 - +3.2 1,349,877 + -1.8 1,349,877 + -2.5 1,349,925 + +1.3 1,349,925 + +1.9 1,349,925 + -2.6 1,349,926 - -1.5 1,349,926 - +0.6 1,349,926 - -0.2
Or see this region's nucleotide sequence