Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_06045 and AO353_06050 overlap by 4 nucleotides AO353_06050 and AO353_06055 are separated by 154 nucleotides AO353_06055 and AO353_06060 are separated by 146 nucleotides
AO353_06045: AO353_06045 - exodeoxyribonuclease V subunit beta, at 1,342,697 to 1,346,386
_06045
AO353_06050: AO353_06050 - exodeoxyribonuclease V subunit gamma, at 1,346,383 to 1,349,835
_06050
AO353_06055: AO353_06055 - 5S ribosomal RNA, at 1,349,990 to 1,350,105
_06055
AO353_06060: AO353_06060 - 23S ribosomal RNA, at 1,350,252 to 1,353,164
_06060
Position (kb)
1346
1347
1348
1349
1350 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1345.561 kb on + strand, within AO353_06045 at 1345.590 kb on + strand, within AO353_06045 at 1346.284 kb on - strand at 1346.361 kb on - strand at 1346.492 kb on + strand at 1346.492 kb on + strand at 1346.659 kb on - strand at 1346.736 kb on + strand, within AO353_06050 at 1346.736 kb on + strand, within AO353_06050 at 1347.333 kb on - strand, within AO353_06050 at 1347.333 kb on - strand, within AO353_06050 at 1347.333 kb on - strand, within AO353_06050 at 1347.402 kb on - strand, within AO353_06050 at 1347.402 kb on - strand, within AO353_06050 at 1347.402 kb on - strand, within AO353_06050 at 1347.602 kb on + strand, within AO353_06050 at 1347.603 kb on - strand, within AO353_06050 at 1347.603 kb on - strand, within AO353_06050 at 1347.603 kb on - strand, within AO353_06050 at 1347.640 kb on + strand, within AO353_06050 at 1347.640 kb on + strand, within AO353_06050 at 1347.798 kb on + strand, within AO353_06050 at 1347.965 kb on + strand, within AO353_06050 at 1348.139 kb on + strand, within AO353_06050 at 1348.140 kb on - strand, within AO353_06050 at 1348.438 kb on + strand, within AO353_06050 at 1348.541 kb on + strand, within AO353_06050 at 1348.735 kb on + strand, within AO353_06050 at 1348.796 kb on - strand, within AO353_06050 at 1348.796 kb on - strand, within AO353_06050 at 1348.796 kb on - strand, within AO353_06050 at 1348.830 kb on - strand, within AO353_06050 at 1348.847 kb on + strand, within AO353_06050 at 1348.848 kb on - strand, within AO353_06050 at 1348.868 kb on + strand, within AO353_06050 at 1348.869 kb on - strand, within AO353_06050 at 1348.939 kb on + strand, within AO353_06050 at 1348.976 kb on + strand, within AO353_06050 at 1349.037 kb on + strand, within AO353_06050 at 1349.140 kb on + strand, within AO353_06050 at 1349.141 kb on - strand, within AO353_06050 at 1349.415 kb on + strand, within AO353_06050 at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.803 kb on - strand at 1349.864 kb on + strand at 1349.864 kb on + strand at 1349.865 kb on - strand at 1349.865 kb on - strand at 1349.877 kb on + strand at 1349.877 kb on + strand at 1349.925 kb on + strand at 1349.925 kb on + strand at 1349.925 kb on + strand at 1349.926 kb on - strand at 1349.926 kb on - strand at 1349.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,345,561 + AO353_06045 0.78 -0.0 1,345,590 + AO353_06045 0.78 -1.1 1,346,284 - -0.4 1,346,361 - +2.0 1,346,492 + -1.5 1,346,492 + +0.3 1,346,659 - +1.0 1,346,736 + AO353_06050 0.10 -0.8 1,346,736 + AO353_06050 0.10 -1.0 1,347,333 - AO353_06050 0.28 -1.9 1,347,333 - AO353_06050 0.28 +2.6 1,347,333 - AO353_06050 0.28 -0.9 1,347,402 - AO353_06050 0.30 +2.4 1,347,402 - AO353_06050 0.30 -0.0 1,347,402 - AO353_06050 0.30 -0.4 1,347,602 + AO353_06050 0.35 -2.6 1,347,603 - AO353_06050 0.35 -0.7 1,347,603 - AO353_06050 0.35 -1.8 1,347,603 - AO353_06050 0.35 +0.9 1,347,640 + AO353_06050 0.36 -0.8 1,347,640 + AO353_06050 0.36 -0.8 1,347,798 + AO353_06050 0.41 -2.5 1,347,965 + AO353_06050 0.46 -1.7 1,348,139 + AO353_06050 0.51 -0.2 1,348,140 - AO353_06050 0.51 -1.3 1,348,438 + AO353_06050 0.60 -1.6 1,348,541 + AO353_06050 0.62 +0.7 1,348,735 + AO353_06050 0.68 -0.7 1,348,796 - AO353_06050 0.70 -1.5 1,348,796 - AO353_06050 0.70 -1.4 1,348,796 - AO353_06050 0.70 -0.5 1,348,830 - AO353_06050 0.71 -1.7 1,348,847 + AO353_06050 0.71 -0.7 1,348,848 - AO353_06050 0.71 -2.0 1,348,868 + AO353_06050 0.72 -0.7 1,348,869 - AO353_06050 0.72 -0.4 1,348,939 + AO353_06050 0.74 -1.0 1,348,976 + AO353_06050 0.75 +0.2 1,349,037 + AO353_06050 0.77 -1.5 1,349,140 + AO353_06050 0.80 -0.0 1,349,141 - AO353_06050 0.80 -0.7 1,349,415 + AO353_06050 0.88 -0.2 1,349,803 - -0.7 1,349,803 - +1.3 1,349,803 - -2.0 1,349,803 - +0.9 1,349,864 + -1.2 1,349,864 + -0.2 1,349,865 - +0.7 1,349,865 - +3.2 1,349,877 + -1.8 1,349,877 + -2.5 1,349,925 + +1.3 1,349,925 + +1.9 1,349,925 + -2.6 1,349,926 - -1.5 1,349,926 - +0.6 1,349,926 - -0.2
Or see this region's nucleotide sequence