Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02275 and AO353_02280 are separated by 75 nucleotides AO353_02280 and AO353_02285 are separated by 77 nucleotides AO353_02285 and AO353_02290 are separated by 103 nucleotides AO353_02290 and AO353_02295 are separated by 194 nucleotides
AO353_02275: AO353_02275 - hypothetical protein, at 486,116 to 486,436
_02275
AO353_02280: AO353_02280 - DNA mismatch repair protein MutS, at 486,512 to 487,069
_02280
AO353_02285: AO353_02285 - GTP cyclohydrolase, at 487,147 to 487,692
_02285
AO353_02290: AO353_02290 - glutaredoxin, at 487,796 to 488,167
_02290
AO353_02295: AO353_02295 - GntR family transcriptional regulator, at 488,362 to 489,528
_02295
Position (kb) 487
488 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 487.025 kb on + strand at 487.025 kb on + strand at 487.025 kb on + strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.067 kb on + strand at 487.067 kb on + strand at 487.068 kb on - strand at 487.068 kb on - strand at 487.690 kb on + strand at 487.690 kb on + strand at 487.690 kb on + strand at 487.690 kb on + strand at 487.691 kb on - strand at 487.691 kb on - strand at 487.691 kb on - strand at 487.701 kb on - strand at 487.754 kb on + strand at 487.755 kb on - strand at 487.755 kb on - strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.790 kb on + strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.791 kb on - strand at 487.792 kb on + strand at 487.792 kb on + strand at 487.792 kb on + strand at 487.792 kb on + strand at 487.793 kb on - strand at 487.793 kb on - strand at 487.793 kb on - strand at 487.800 kb on + strand at 487.800 kb on + strand at 487.801 kb on - strand at 487.801 kb on - strand at 487.801 kb on - strand at 487.804 kb on - strand at 487.804 kb on - strand at 487.932 kb on - strand, within AO353_02290 at 487.932 kb on - strand, within AO353_02290 at 488.104 kb on + strand, within AO353_02290 at 488.105 kb on - strand, within AO353_02290 at 488.105 kb on - strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.114 kb on + strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.115 kb on - strand, within AO353_02290 at 488.138 kb on + strand at 488.138 kb on + strand at 488.139 kb on - strand at 488.139 kb on - strand at 488.139 kb on - strand at 488.216 kb on + strand at 488.216 kb on + strand at 488.216 kb on + strand at 488.216 kb on + strand at 488.217 kb on - strand at 488.217 kb on - strand at 488.262 kb on + strand at 488.304 kb on + strand at 488.305 kb on - strand at 488.320 kb on + strand at 488.343 kb on - strand at 488.343 kb on - strand at 488.352 kb on + strand at 488.352 kb on + strand at 488.395 kb on + strand at 488.396 kb on - strand at 488.396 kb on - strand at 488.396 kb on - strand at 488.412 kb on + strand at 488.448 kb on + strand at 488.511 kb on + strand, within AO353_02295 at 488.511 kb on + strand, within AO353_02295 at 488.511 kb on + strand, within AO353_02295 at 488.512 kb on - strand, within AO353_02295 at 488.605 kb on + strand at 488.605 kb on + strand, within AO353_02295 at 488.605 kb on + strand, within AO353_02295 at 488.605 kb on + strand, within AO353_02295 at 488.605 kb on + strand, within AO353_02295
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 486,341 + AO353_02275 0.70 -0.2 486,341 + AO353_02275 0.70 -0.9 486,341 + AO353_02275 0.70 +1.0 486,341 + AO353_02275 0.70 -0.8 486,341 + AO353_02275 0.70 +1.3 486,341 + AO353_02275 0.70 -0.0 486,342 - AO353_02275 0.70 +0.3 486,342 - AO353_02275 0.70 +1.6 486,342 - AO353_02275 0.70 -1.3 486,342 - AO353_02275 0.70 -0.0 486,342 - AO353_02275 0.70 +2.1 486,342 - AO353_02275 0.70 +1.1 486,906 + AO353_02280 0.71 +1.1 486,906 + AO353_02280 0.71 +0.6 486,906 + AO353_02280 0.71 +1.0 486,906 + AO353_02280 0.71 -0.6 487,025 + +0.4 487,025 + -1.0 487,025 + -0.2 487,026 - +0.7 487,026 - +1.5 487,026 - -1.1 487,026 - -2.0 487,026 - +0.5 487,067 + +1.0 487,067 + +1.6 487,068 - -0.7 487,068 - -2.0 487,690 + +0.0 487,690 + -0.2 487,690 + -2.1 487,690 + -0.7 487,691 - -2.1 487,691 - -0.4 487,691 - +0.8 487,701 - -0.5 487,754 + +0.3 487,755 - +0.4 487,755 - -1.7 487,790 + -0.5 487,790 + +1.6 487,790 + +0.3 487,790 + +1.3 487,790 + -0.6 487,790 + -2.0 487,790 + -0.4 487,790 + +1.4 487,790 + +0.1 487,791 - -0.4 487,791 - +1.9 487,791 - +1.0 487,791 - -1.6 487,791 - +0.8 487,791 - +1.4 487,791 - +2.8 487,791 - +1.0 487,791 - -0.2 487,791 - -1.5 487,791 - +0.8 487,791 - -2.1 487,792 + +1.6 487,792 + -0.7 487,792 + -1.5 487,792 + -1.8 487,793 - -1.3 487,793 - -0.6 487,793 - -0.6 487,800 + -1.1 487,800 + +0.3 487,801 - -2.1 487,801 - +1.3 487,801 - -2.2 487,804 - -0.0 487,804 - +0.9 487,932 - AO353_02290 0.37 +1.0 487,932 - AO353_02290 0.37 -0.7 488,104 + AO353_02290 0.83 -1.4 488,105 - AO353_02290 0.83 -0.3 488,105 - AO353_02290 0.83 +2.2 488,114 + AO353_02290 0.85 -0.2 488,114 + AO353_02290 0.85 +0.7 488,114 + AO353_02290 0.85 -0.1 488,114 + AO353_02290 0.85 -2.3 488,114 + AO353_02290 0.85 -0.7 488,114 + AO353_02290 0.85 -0.9 488,114 + AO353_02290 0.85 +0.7 488,114 + AO353_02290 0.85 +0.8 488,114 + AO353_02290 0.85 +0.4 488,114 + AO353_02290 0.85 -2.6 488,114 + AO353_02290 0.85 -0.6 488,114 + AO353_02290 0.85 -0.2 488,114 + AO353_02290 0.85 +1.4 488,114 + AO353_02290 0.85 -0.2 488,115 - AO353_02290 0.86 -2.4 488,115 - AO353_02290 0.86 +2.4 488,115 - AO353_02290 0.86 -1.9 488,115 - AO353_02290 0.86 +2.0 488,115 - AO353_02290 0.86 -0.5 488,115 - AO353_02290 0.86 -0.1 488,115 - AO353_02290 0.86 +1.1 488,115 - AO353_02290 0.86 -0.9 488,115 - AO353_02290 0.86 +0.8 488,115 - AO353_02290 0.86 -1.3 488,138 + -0.1 488,138 + -2.6 488,139 - +2.3 488,139 - -2.3 488,139 - +0.7 488,216 + +2.1 488,216 + +0.5 488,216 + +0.9 488,216 + +0.3 488,217 - +1.6 488,217 - -0.4 488,262 + -0.7 488,304 + -2.1 488,305 - -1.1 488,320 + +0.6 488,343 - -0.1 488,343 - -2.2 488,352 + +0.3 488,352 + +0.3 488,395 + +1.6 488,396 - -0.4 488,396 - +0.8 488,396 - +2.3 488,412 + +0.7 488,448 + +1.4 488,511 + AO353_02295 0.13 +1.0 488,511 + AO353_02295 0.13 +1.9 488,511 + AO353_02295 0.13 +0.4 488,512 - AO353_02295 0.13 -1.0 488,605 + +1.6 488,605 + AO353_02295 0.21 +1.5 488,605 + AO353_02295 0.21 -1.3 488,605 + AO353_02295 0.21 +1.0 488,605 + AO353_02295 0.21 -0.3
Or see this region's nucleotide sequence