Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08425 and MMP_RS08430 overlap by 11 nucleotides MMP_RS08430 and MMP_RS08435 are separated by 76 nucleotides MMP_RS08435 and MMP_RS08440 are separated by 6 nucleotides MMP_RS08440 and MMP_RS08445 are separated by 24 nucleotides
MMP_RS08425: MMP_RS08425 - methionine adenosyltransferase, at 1,582,001 to 1,583,218
_RS08425
MMP_RS08430: MMP_RS08430 - HAD family hydrolase, at 1,583,208 to 1,584,014
_RS08430
MMP_RS08435: MMP_RS08435 - Era-like GTP-binding protein, at 1,584,091 to 1,584,645
_RS08435
MMP_RS08440: MMP_RS08440 - cell division protein SepF, at 1,584,652 to 1,585,041
_RS08440
MMP_RS08445: MMP_RS08445 - DUF2099 family protein, at 1,585,066 to 1,585,917
_RS08445
Position (kb)
1584
1585 Strain fitness (log2 ratio)
-1
0
1 at 1583.206 kb on + strand at 1583.206 kb on + strand at 1583.209 kb on - strand at 1583.209 kb on - strand at 1583.209 kb on - strand at 1583.210 kb on + strand at 1583.210 kb on + strand at 1583.217 kb on + strand at 1583.253 kb on - strand at 1583.254 kb on + strand at 1583.256 kb on + strand at 1583.263 kb on + strand at 1583.347 kb on + strand, within MMP_RS08430 at 1583.621 kb on - strand, within MMP_RS08430 at 1583.622 kb on + strand, within MMP_RS08430 at 1583.624 kb on + strand, within MMP_RS08430 at 1583.624 kb on + strand, within MMP_RS08430 at 1583.628 kb on - strand, within MMP_RS08430 at 1583.646 kb on + strand, within MMP_RS08430 at 1583.646 kb on + strand, within MMP_RS08430 at 1583.967 kb on - strand at 1583.973 kb on - strand at 1583.974 kb on + strand at 1584.034 kb on + strand at 1584.036 kb on - strand at 1584.037 kb on + strand at 1584.037 kb on + strand at 1584.055 kb on - strand at 1584.058 kb on - strand at 1584.060 kb on - strand at 1584.068 kb on + strand at 1584.310 kb on - strand, within MMP_RS08435 at 1584.324 kb on - strand, within MMP_RS08435 at 1584.422 kb on - strand, within MMP_RS08435 at 1584.481 kb on + strand, within MMP_RS08435 at 1584.593 kb on + strand at 1584.614 kb on - strand at 1584.714 kb on - strand, within MMP_RS08440 at 1584.715 kb on + strand, within MMP_RS08440 at 1584.815 kb on - strand, within MMP_RS08440 at 1584.816 kb on + strand, within MMP_RS08440 at 1584.948 kb on + strand, within MMP_RS08440 at 1585.063 kb on - strand at 1585.453 kb on + strand, within MMP_RS08445 at 1585.453 kb on + strand, within MMP_RS08445 at 1585.470 kb on + strand, within MMP_RS08445
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,583,206 + -0.7 1,583,206 + +0.3 1,583,209 - -0.1 1,583,209 - -0.1 1,583,209 - -0.6 1,583,210 + -0.2 1,583,210 + -0.1 1,583,217 + +0.2 1,583,253 - -0.3 1,583,254 + +0.5 1,583,256 + -0.0 1,583,263 + -0.3 1,583,347 + MMP_RS08430 0.17 +0.1 1,583,621 - MMP_RS08430 0.51 -0.2 1,583,622 + MMP_RS08430 0.51 -0.1 1,583,624 + MMP_RS08430 0.52 +0.1 1,583,624 + MMP_RS08430 0.52 +0.0 1,583,628 - MMP_RS08430 0.52 +0.1 1,583,646 + MMP_RS08430 0.54 -0.5 1,583,646 + MMP_RS08430 0.54 +0.9 1,583,967 - +0.1 1,583,973 - +0.1 1,583,974 + +0.5 1,584,034 + -0.1 1,584,036 - +0.6 1,584,037 + -0.1 1,584,037 + +0.2 1,584,055 - -0.5 1,584,058 - -0.3 1,584,060 - -1.1 1,584,068 + +0.1 1,584,310 - MMP_RS08435 0.39 -0.4 1,584,324 - MMP_RS08435 0.42 -1.3 1,584,422 - MMP_RS08435 0.60 -0.8 1,584,481 + MMP_RS08435 0.70 -0.9 1,584,593 + -0.8 1,584,614 - -0.8 1,584,714 - MMP_RS08440 0.16 +0.5 1,584,715 + MMP_RS08440 0.16 -0.0 1,584,815 - MMP_RS08440 0.42 -0.1 1,584,816 + MMP_RS08440 0.42 +1.0 1,584,948 + MMP_RS08440 0.76 -0.2 1,585,063 - -0.5 1,585,453 + MMP_RS08445 0.45 +0.1 1,585,453 + MMP_RS08445 0.45 -0.4 1,585,470 + MMP_RS08445 0.47 +0.2
Or see this region's nucleotide sequence